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Development of rapid dipstick assay for food pathogens, Salmonella, by optimized parameters

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Abstract

Salmonella is among the very important pathogens threating the human and animal health. Rapid and easy detection of these pathogens is crucial. In this context, antibody (Ab) based lateral flow assays (LFAs) which are simple immunochromatographic point of care test kits were developed by gold nanoparticles (GNPs) as labelling agent for Salmonella detection. For that purpose some critical parameters such as reagent concentrations on the capture zones, conjugate concentrations and ideal membrane type needed for LFAs for whole cell detection were tested for naked eye analysis. Therefore, prepared LFAs were applied to the live and heat inactivated cells when they were used alone or included in different bacterial mixtures. Among the test platforms, membrane 180 (M180) was found as an ideal membrane and 36 nm GNPs showed highly good labelling in the developed LFAs. Diluted conjugates and low concentrations of reagents affected the test signal negatively. Salmonella was detected in different bacterial mixtures, selectively in 4–5 min. The best recognized species by used Ab were S. enteritidis and S. infantis. 5 × 105S. typhimurium cells were also determined as a limit of detection of this study with mentioned parameters.

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Acknowledgements

This research was supported by the Faculty Development Program (ÖYP-BAP-08-11-DPT-2011K121010), and Scientific and Technological Research Council of Turkey (TÜBİTAK-TEYDEB) projects funded to Nanobiz Nanobiotechnological Systems R&D Limited. The authors acknowledge to Ankara University Faculty of Veterinary Medicine for bacterial samples and Dr. Ceren Berkman for help.

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Authors and Affiliations

  1. Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey

    Dilek Çam & Hüseyin Avni Öktem

  2. Department of Biology, Çankırı Karatekin University, 18100, Çankırı, Turkey

    Dilek Çam

  3. NANOBIZ TECHNOLOGY INC., Gallium Block No: 27/218, METU Technopolis, 06800, Ankara, Turkey

    Hüseyin Avni Öktem

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  1. Dilek Çam
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Correspondence to Dilek Çam.

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Çam, D., Öktem, H.A. Development of rapid dipstick assay for food pathogens, Salmonella, by optimized parameters. J Food Sci Technol 56, 140–148 (2019). https://doi.org/10.1007/s13197-018-3467-5

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