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Exploring diverse wheat germplasm for novel alleles in HMW-GS for bread quality improvement

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Abstract

Wheat is one of the most important cereals used worldwide in the form of a range of products. Crop landraces have been an immense source of diversity for the breeders. In the present study, 517 Indian wheat landraces have been observed for the difference in bread making quality by assessing allelic behaviour of high molecular weight-glutenin subunits (HMW-GS). A total of 33 Glu-1 alleles (3 at Glu-A1, 15 at Glu-B1 and 15 at Glu-D1) were detected in wheat landraces. Allelic frequency of HMW-GS allelic band pattern null, 17 + 18, 2 + 12 (24.75%) was found to be the highest. Allelic frequency of HMW-GS allele null (68.27%) at Glu-A1, 17 + 18 (49.14%) at Glu-B1, and 2 + 12 (72.81%) at Glu-D1 was found to be the highest Five Novel alleles were identified at Glu-D1 locus, 12*, 12.1, 12.1*, 12.2 and 12.3. As Glu-D1 has highest quality contribution as compared to Glu-A1 and Glu-B1, reporting novel alleles at Glu-D1 represents that genetic variability available for selection is increased and it will provide tools for breeders to further improve dough properties and bread making quality.

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Acknowledgements

We are grateful to the ICAR Network Project on Transgenics in Crops (NPTC) for providing funding support. The authors would furthermore like to thank National Beaureu of Plant genetic resources, Delhi for providing wheat germplasm.

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Authors and Affiliations

  1. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India

    Sonia Goel, Ranjeet Singh Jaat & N. K. Singh

  2. Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India

    Mohini Yadav

  3. Water Technology Centre, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India

    Kalpana Singh

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  1. Sonia Goel
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  2. Mohini Yadav
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  3. Kalpana Singh
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  4. Ranjeet Singh Jaat
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  5. N. K. Singh
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Correspondence to Sonia Goel.

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Goel, S., Yadav, M., Singh, K. et al. Exploring diverse wheat germplasm for novel alleles in HMW-GS for bread quality improvement. J Food Sci Technol 55, 3257–3262 (2018). https://doi.org/10.1007/s13197-018-3259-y

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  • DOI: https://doi.org/10.1007/s13197-018-3259-y

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