Optimization of microwave assisted extraction of essential oils from Iranian Rosmarinus officinalis L. using RSM
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In this study, the efficiencies of conventional hydro-distillation and novel microwave hydro-distillation methods in extraction of essential oil from Rosemary officinalis leaves have been compared. In order to attain the best yield and also highest quality of the essential oil in the microwave assisted method, the optimal values of operating parameters such as extraction time, microwave irradiation power and water volume to plant mass ratio were investigated using central composite design under response surface methodology. Optimal conditions for obtaining the maximum extraction yield in the microwave assisted method were predicted as follows: extraction time of 85 min, microwave power of 888 W, and water volume to plant mass ratio of 0.5 ml/g. The extraction yield at these predicted conditions was computed as 0.7756%. The qualities of the obtained essential oils under designed experiments were optimized based on total contents of four major compounds (α-pinene, 1,8-cineole, camphor and verbenone) which determined by gas chromatography equipped with mass spectroscopy (GC–MS). The highest essential oil quality (55.87%) was obtained at extraction time of 68 min; microwave irradiation power of 700 W; and water volume to plant mass ratio of zero.
KeywordsRosemary officinalis L. Quality assessment of essential oil Gas chromatography–mass spectroscopy Response surface methodology Central composite design
The authors would like to thank University of Kashan for its financial supports.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- Bousbia N, Abert Vian M, Ferhat MA, Petitcolas E, Meklati BY, Chemat F (2009) Comparison of two isolation methods for essential oil from rosemary leaves: hydrodistillation and microwave hydrodiffusion and gravity. Food Chem 114:355–362. https://doi.org/10.1016/j.foodchem.2008.09.106 CrossRefGoogle Scholar
- Chen F, Du X, Zu Y, Yang L, Wang F (2016) Microwave-assisted method for distillation and dual extraction in obtaining essential oil, proanthocyanidins and polysaccharides by one-pot process from Cinnamomi Cortex. Sep Purif Technol 164:1–11. https://doi.org/10.1016/j.seppur.2016.03.018 CrossRefGoogle Scholar
- Chen F, Xu M, Yang X, Liu J, Xiao Y, Yang L (2018) An improved approach for the isolation of essential oil from the leaves of Cinnamomum longepaniculatum using microwave-assisted hydrodistillation concatenated double-column liquid-liquid extraction. Sep Purif Technol 195:110–120. https://doi.org/10.1016/j.seppur.2017.12.013 CrossRefGoogle Scholar
- González-Rivera J, Duce C, Falconieri D, Ferrari C, Ghezzi L, Piras A, Tine MR (2016) Coaxial microwave assisted hydrodistillation of essential oils from five different herbs (lavender, rosemary, sage, fennel seeds and clove buds): chemical composition and thermal analysis. Innov Food Sci Emerg Technol 33:308–318. https://doi.org/10.1016/j.ifset.2015.12.011 CrossRefGoogle Scholar
- Hashemimoghadam H, Feyzi P, Kamali H, Nematollahi A (2013) Comparison of microwave extraction and hydrodistillation of essential oil from Biebersteinia multifida DC. Conjunction with gas chromatography–mass spectroscopy: optimization via Box-Behnken. JNKUMS 4:13–21. https://doi.org/10.29252/jnkums.4.5.S5.13 CrossRefGoogle Scholar
- Karabegović IT, Stojičević SS, Veličković DT, Nikolić NČ, Lazić ML (2013) Optimization of microwave-assisted extraction and characterization of phenolic compounds in cherry laurel (Prunus laurocerasus) leaves. Sep Purif Technol 120:429–436. https://doi.org/10.1016/j.seppur.2013.10.021 CrossRefGoogle Scholar
- Mezza GN, Borgarello AV, Grosso NR, Fernandez H, Pramparo MC, Gayol MF (2018) Antioxidant activity of rosemary essential oil fractions obtained by molecular distillation and their effect on oxidative stability of sunflower oil. Food Chem 242:9–15. https://doi.org/10.1016/j.foodchem.2017.09.042 CrossRefPubMedGoogle Scholar
- Ranjbar Nedamani E, Sadeghi Mahoonak A, Ghorbani M, Kashaninejad M (2015) Evaluation of antioxidant interactions in combined extracts of green tea (Camellia sinensis), rosemary (Rosmarinus officinalis) and oak fruit (Quercus branti). J Food Sci Technol 52:4565–4571. https://doi.org/10.1007/s13197-014-1497-1 CrossRefPubMedGoogle Scholar