Acta Oceanologica Sinica

, Volume 37, Issue 2, pp 110–118 | Cite as

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21 (DE3) through induction mode optimization and glycerol feeding strategy

  • Zhuhua Chan
  • Xinglin Chen
  • Yanping Hou
  • Boliang Gao
  • Chungui Zhao
  • Suping Yang
  • Runying Zeng
Article

Abstract

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food, cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates (μ) was applied in the pre-induction phase. The results showed that the low μ (μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside (IPTG), the strategy of 0.8 mmol/L IPTG induction at 20°C was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date. Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose (NA4) and neoagarohexaose (NA6). The overall research may be useful for the industrial production and application of β-agarase.

Keywords

β-agarase Escherichia coli process optimization glycerol feeding strategy neoagarooligosaccharide 

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Copyright information

© The Chinese Society of Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Zhuhua Chan
    • 1
    • 2
  • Xinglin Chen
    • 2
  • Yanping Hou
    • 2
  • Boliang Gao
    • 2
    • 3
  • Chungui Zhao
    • 1
  • Suping Yang
    • 1
  • Runying Zeng
    • 2
  1. 1.Department of Bioengineering and BiotechnologyHuaqiao UniversityXiamenChina
  2. 2.State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of OceanographyState Oceanic AdministrationXiamenChina
  3. 3.School of Life SciencesXiamen UniversityXiamenChina

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