Herpud1 deficiency could reduce amyloid-β40 expression and thereby suppress homocysteine-induced atherosclerosis by blocking the JNK/AP1 pathway

Abstract

Homocysteine (Hcy) is considered an independent risk factor for various cardiovascular diseases including atherosclerosis which is associated with lipid metabolism, inflammation, and oxidative stress. Results from our previous study suggested that Hcy-induced atherosclerosis could be reversed by Herpud1 knockout which inhibits vascular smooth muscle cell (VSMC) phenotype switching. Here, we aim to investigate more precise mechanisms behind the improvement in Hcy-induced atherosclerosis. Amyloid-β40 (Aβ40), a vital protein in Alzheimer disease (AD), has been regarded as an important component in the atherosclerosis program in recent years due to the biological similarity between AD and atherosclerosis. Thus, we determined to assess the value of Aβ40 in a Herpud1 knockout Hcy-induced atherosclerosis mouse model by measuring Aβ40 expression in tissue and biomarkers of lipid metabolism, inflammation, and oxidative stress in serum. Additionally, since endothelial dysfunction plays a prominent role in atherosclerosis, we tested human umbilical vein endothelial cell (HUVEC) function following Herpud1 silencing in vitro and evaluated JNK/AP1 signaling activation in our models because of its close relationship with Aβ40. As a result, our animal models showed that Herpud1 knockout reduced Aβ40 expression, inflammation, and oxidative stress levels other than lipid metabolism and alleviated atherosclerosis via JNK/AP1 signaling inhibition. Similarly, our cell experiments implied that Hcy-induced Aβ40 elevation and HUVEC dysfunction involving cell proliferation and apoptosis could be restored by Herpud1 silence through restraining JNK/AP1 pathway. Collectively, our study demonstrates that Herpud1 deficiency could reduce Aβ40 expression, thereby suppressing Hcy-induced atherosclerosis by blocking the JNK/AP1 pathway. This may provide novel potential targets for atherosclerosis prevention or treatment.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. 1.

    Akhter R, Sanphui P, Das H, Saha P, Biswas SC (2015) The regulation of p53 up-regulated modulator of apoptosis by JNK/c-Jun pathway in beta-amyloid-induced neuron death. J Neurochem 134:1091–1103. https://doi.org/10.1111/jnc.13128

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Audagnotto M, Kengo Lorkowski A, Dal Peraro M (2018) Recruitment of the amyloid precursor protein by gamma-secretase at the synaptic plasma membrane. Biochem Biophys Res Commun 498:334–341. https://doi.org/10.1016/j.bbrc.2017.10.164

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Austin SA, Combs CK (2010) Amyloid precursor protein mediates monocyte adhesion in AD tissue and apoE(−)/(−) mice. Neurobiol Aging 31:1854–1866. https://doi.org/10.1016/j.neurobiolaging.2008.10.013

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Bamji-Mirza M, Callaghan D, Najem D, Shen S, Hasim MS, Yang Z, Zhang W (2014) Stimulation of insulin signaling and inhibition of JNK-AP1 activation protect cells from amyloid-beta-induced signaling dysregulation and inflammatory response. J Alzheimers Dis 40:105–122. https://doi.org/10.3233/JAD-131949

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Biberoglu K, Yuksel M, Tacal O (2019) Azure B affects amyloid precursor protein metabolism in PS70cells. Chem Biol Interact 299:88–93. https://doi.org/10.1016/j.cbi.2018.11.023

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Boushey CJ, Beresford SA, Omenn GS, Motulsky AG (1995) A quantitative assessment of plasma homocysteine as a risk factor for vascular disease. Probable benefits of increasing folic acid intakes. JAMA 274:1049–1057. https://doi.org/10.1001/jama.1995.03530130055028

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Chandra D, Gupta A, Strollo PJ Jr, Fuhrman CR, Leader JK, Bon J, Slivka WA, Shoushtari AH, Avolio J, Kip KE, Reis S, Sciurba FC (2016) Airflow limitation and endothelial dysfunction. Unrelated and independent predictors of atherosclerosis. Am J Respir Crit Care Med 194:38–47. https://doi.org/10.1164/rccm.201510-2093OC

    Article  PubMed  PubMed Central  Google Scholar 

  8. 8.

    Chang X, Salim A, Dorajoo R, Han Y, Khor CC, van Dam RM, Yuan JM, Koh WP, Liu J, Goh DY, Wang X, Teo YY, Friedlander Y, Heng CK (2017) Utility of genetic and non-genetic risk factors in predicting coronary heart disease in Singaporean Chinese. Eur J Prev Cardiol 24:153–160. https://doi.org/10.1177/2047487316676609

    Article  PubMed  Google Scholar 

  9. 9.

    Dekou V, Whincup P, Papacosta O, Ebrahim S, Lennon L, Ueland PM, Refsum H, Humphries SE, Gudnason V (2001) The effect of the C677T and A1298C polymorphisms in the methylenetetrahydrofolate reductase gene on homocysteine levels in elderly men and women from the British regional heart study. Atherosclerosis 154:659–666

    CAS  Article  Google Scholar 

  10. 10.

    Ding W, Chen R, Wu C, Chen W, Zhang H, Fan X, Wang H, Ji Y, Xie L, Ning X, Shen L (2017) Increased expression of HERPUD1 involves in neuronal apoptosis after intracerebral hemorrhage. Brain Res Bull 128:40–47. https://doi.org/10.1016/j.brainresbull.2016.11.006

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Ding X, Wang X, Zhu X, Zhang J, Zhu Y, Shao X, Zhou X (2020) JNK/AP1 pathway regulates MYC expression and BCR signaling through Ig enhancers in Burkitt lymphoma cells. J Cancer 11:610–618. https://doi.org/10.7150/jca.34055

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. 12.

    Fredman G (2019) Resolving atherosclerosis and Alzheimer disease. Nat Rev Cardiol 16:259–260. https://doi.org/10.1038/s41569-019-0182-5

    Article  PubMed  Google Scholar 

  13. 13.

    Guo HY, Xu FK, Lv HT, Liu LB, Ji Z, Zhai XY, Tang WL, Chi JF (2014) Hyperhomocysteinemia independently causes and promotes atherosclerosis in LDL receptor-deficient mice. J Geriatr Cardiol 11:74–78. https://doi.org/10.3969/j.issn.1671-5411.2014.01.013

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Gupta A, Iadecola C (2015) Impaired Abeta clearance: a potential link between atherosclerosis and Alzheimer’s disease. Front Aging Neurosci 7:115. https://doi.org/10.3389/fnagi.2015.00115

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. 15.

    He G, Qing H, Cai F, Kwok C, Xu H, Yu G, Bernstein A, Song W (2006) Ubiquitin-proteasome pathway mediates degradation of APH-1. J Neurochem 99:1403–1412. https://doi.org/10.1111/j.1471-4159.2006.04184.x

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Hu C, Xu J, Zeng L, Li T, Cui MZ, Xu X (2017) Pen-2 and presenilin are sufficient to catalyze notch processing. J Alzheimers Dis 56:1263–1269. https://doi.org/10.3233/JAD-161094

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  17. 17.

    Jin E, Bai Y, Huang L, Zhao M, Zhang C, Zhao M, Li X (2015) Evidence of a novel gene HERPUD1 in polypoidal choroidal vasculopathy. Int J Clin Exp Pathol 8:13928–13944

    CAS  PubMed  PubMed Central  Google Scholar 

  18. 18.

    Kokjohn TA, Van Vickle GD, Maarouf CL, Kalback WM, Hunter JM, Daugs ID, Luehrs DC, Lopez J, Brune D, Sue LI, Beach TG, Castano EM, Roher AE (2011) Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets. Biochim Biophys Acta 1812:1508–1514. https://doi.org/10.1016/j.bbadis.2011.07.004

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. 19.

    Krishna SM, Seto SW, Jose RJ, Biros E, Moran CS, Wang Y, Clancy P, Golledge J (2015) A peptide antagonist of thrombospondin-1 promotes abdominal aortic aneurysm progression in the angiotensin II-infused apolipoprotein-E-deficient mouse. Arterioscler Thromb Vasc Biol 35:389–398. https://doi.org/10.1161/ATVBAHA.114.304732

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Li JK, Nie L, Zhao YP, Zhang YQ, Wang X, Wang SS, Liu Y, Zhao H, Cheng L (2016) IL-17 mediates inflammatory reactions via p38/c-Fos and JNK/c-Jun activation in an AP-1-dependent manner in human nucleus pulposus cells. J Transl Med 14:77. https://doi.org/10.1186/s12967-016-0833-9

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Li J, Zhang Y, Zhang Y, Lu S, Miao Y, Yang J, Huang S, Ma X, Han L, Deng J, Fan F, Liu B, Huo Y, Xu Q, Chen C, Wang X, Feng J (2018) GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation. Redox Biol 17:386–399. https://doi.org/10.1016/j.redox.2018.04.021

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. 22.

    Lin H, Ni T, Zhang J, Meng L, Gao F, Pan S, Luo H, Xu F, Ru G, Chi J, Guo H (2018) Knockdown of Herp alleviates hyperhomocysteinemia mediated atherosclerosis through the inhibition of vascular smooth muscle cell phenotype switching. Int J Cardiol 269:242–249. https://doi.org/10.1016/j.ijcard.2018.07.043

    Article  PubMed  Google Scholar 

  23. 23.

    Lin H, Ni T, Zhang J, Meng L, Gao F, Pan S, Luo H, Xu F, Ru G, Chi J, Guo H (2019) Corrigendum to "Knockdown of Herp alleviates hyperhomocysteinemia mediated atherosclerosis through the inhibition of vascular smooth muscle cell phenotype switching" [Int. J. Cardiol. 269 (2018) 242-249]. Int J Cardiol 278:310. https://doi.org/10.1016/j.ijcard.2018.10.035

    Article  PubMed  Google Scholar 

  24. 24.

    Mehlig K, Leander K, de Faire U, Nyberg F, Berg C, Rosengren A, Bjorck L, Zetterberg H, Blennow K, Tognon G, Toren K, Strandhagen E, Lissner L, Thelle D (2013) The association between plasma homocysteine and coronary heart disease is modified by the MTHFR 677C>T polymorphism. Heart 99:1761–1765. https://doi.org/10.1136/heartjnl-2013-304460

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Navarro-Marquez M, Torrealba N, Troncoso R, Vasquez-Trincado C, Rodriguez M, Morales PE, Villalobos E, Eura Y, Garcia L, Chiong M, Klip A, Jaimovich E, Kokame K, Lavandero S (2018) Herpud1 impacts insulin-dependent glucose uptake in skeletal muscle cells by controlling the Ca(2+)-calcineurin-Akt axis. Biochim Biophys Acta Mol basis Dis 1864:1653–1662. https://doi.org/10.1016/j.bbadis.2018.02.018

    CAS  Article  PubMed  Google Scholar 

  26. 26.

    Oh YT, Yue P, Sun SY (2017) DR5 suppression induces sphingosine-1-phosphate-dependent TRAF2 polyubiquitination, leading to activation of JNK/AP-1 and promotion of cancer cell invasion. Cell Commun Signal 15:18. https://doi.org/10.1186/s12964-017-0174-1

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  27. 27.

    Olsson B, Lautner R, Andreasson U, Ohrfelt A, Portelius E, Bjerke M, Holtta M, Rosen C, Olsson C, Strobel G, Wu E, Dakin K, Petzold M, Blennow K, Zetterberg H (2016) CSF and blood biomarkers for the diagnosis of Alzheimer’s disease: a systematic review and meta-analysis. Lancet Neurol 15:673–684. https://doi.org/10.1016/S1474-4422(16)00070-3

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Park HJ, Shabashvili D, Nekorchuk MD, Shyqyriu E, Jung JI, Ladd TB, Moore BD, Felsenstein KM, Golde TE, Kim SH (2012) Retention in endoplasmic reticulum 1 (RER1) modulates amyloid-beta (Abeta) production by altering trafficking of gamma-secretase and amyloid precursor protein (APP). J Biol Chem 287:40629–40640. https://doi.org/10.1074/jbc.M112.418442

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  29. 29.

    Roher AE, Esh CL, Kokjohn TA, Castano EM, Van Vickle GD, Kalback WM, Patton RL, Luehrs DC, Daugs ID, Kuo YM, Emmerling MR, Soares H, Quinn JF, Kaye J, Connor DJ, Silverberg NB, Adler CH, Seward JD, Beach TG, Sabbagh MN (2009) Amyloid beta peptides in human plasma and tissues and their significance for Alzheimer’s disease. Alzheimers Dement 5:18–29. https://doi.org/10.1016/j.jalz.2008.10.004

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  30. 30.

    Sai X, Kawamura Y, Kokame K, Yamaguchi H, Shiraishi H, Suzuki R, Suzuki T, Kawaichi M, Miyata T, Kitamura T, De Strooper B, Yanagisawa K, Komano H (2002) Endoplasmic reticulum stress-inducible protein, Herp, enhances presenilin-mediated generation of amyloid beta-protein. J Biol Chem 277:12915–12920. https://doi.org/10.1074/jbc.M112372200

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    Stamatelopoulos K, Sibbing D, Rallidis LS, Georgiopoulos G, Stakos D, Braun S, Gatsiou A, Sopova K, Kotakos C, Varounis C, Tellis CC, Kastritis E, Alevizaki M, Tselepis AD, Alexopoulos P, Laske C, Keller T, Kastrati A, Dimmeler S, Zeiher AM, Stellos K (2015) Amyloid-beta (1-40) and the risk of death from cardiovascular causes in patients with coronary heart disease. J Am Coll Cardiol 65:904–916. https://doi.org/10.1016/j.jacc.2014.12.035

    CAS  Article  PubMed  Google Scholar 

  32. 32.

    Steiner H (2008) The catalytic core of gamma-secretase: presenilin revisited. Curr Alzheimer Res 5:147–157

    CAS  Article  Google Scholar 

  33. 33.

    Tsang JYS, Lee MA, Chan TH, Li J, Ni YB, Shao Y, Chan SK, Cheungc SY, Lau KF, Tse GMK (2018) Proteolytic cleavage of amyloid precursor protein by ADAM10 mediates proliferation and migration in breast cancer. EBioMedicine 38:89–99. https://doi.org/10.1016/j.ebiom.2018.11.012

    Article  PubMed  PubMed Central  Google Scholar 

  34. 34.

    Tyagi A, Agarwal R, Agarwal C (2003) Grape seed extract inhibits EGF-induced and constitutively active mitogenic signaling but activates JNK in human prostate carcinoma DU145 cells: possible role in antiproliferation and apoptosis. Oncogene 22:1302–1316. https://doi.org/10.1038/sj.onc.1206265

    CAS  Article  PubMed  Google Scholar 

  35. 35.

    Van De Parre TJ, Guns PJ, Fransen P, Martinet W, Bult H, Herman AG, De Meyer GR (2011) Attenuated atherogenesis in apolipoprotein E-deficient mice lacking amyloid precursor protein. Atherosclerosis 216:54–58. https://doi.org/10.1016/j.atherosclerosis.2011.01.032

    CAS  Article  Google Scholar 

  36. 36.

    Vukic V, Callaghan D, Walker D, Lue LF, Liu QY, Couraud PO, Romero IA, Weksler B, Stanimirovic DB, Zhang W (2009) Expression of inflammatory genes induced by beta-amyloid peptides in human brain endothelial cells and in Alzheimer’s brain is mediated by the JNK-AP1 signaling pathway. Neurobiol Dis 34:95–106. https://doi.org/10.1016/j.nbd.2008.12.007

    CAS  Article  PubMed  Google Scholar 

  37. 37.

    Yu J, Ming H, Li HY, Yu B, Chu M, Zhu H, Zhu X (2019) IMM-H007, a novel small molecule inhibitor for atherosclerosis, represses endothelium inflammation by regulating the activity of NF-kappaB and JNK/AP1 signaling. Toxicol Appl Pharmacol 381:114732. https://doi.org/10.1016/j.taap.2019.114732

    CAS  Article  PubMed  Google Scholar 

  38. 38.

    Zhang JY, Jiang H, Gao W, Wu J, Peng K, Shi YF, Zhang XJ (2008) The JNK/AP1/ATF2 pathway is involved in H2O2-induced acetylcholinesterase expression during apoptosis. Cell Mol Life Sci 65:1435–1445. https://doi.org/10.1007/s00018-008-8047-9

    CAS  Article  PubMed  Google Scholar 

  39. 39.

    Zhou PL, Li M, Han XW, Bi YH, Zhang WG, Wu ZY, Wu G (2019) Perilipin 5 deficiency promotes atherosclerosis progression through accelerating inflammation, apoptosis, and oxidative stress. J Cell BiochemJ Cell Biochem 120:19107–19123. https://doi.org/10.1002/jcb.29238

    CAS  Article  Google Scholar 

Download references

Funding

This work was supported by grants from the Zhejiang Medical and Health Project (2018KY172); the National Natural Science Foundation of China (No. 81873120).

Author information

Affiliations

Authors

Corresponding author

Correspondence to Jufang Chi.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Key points

• Herpud1 deficiency could suppress Hcy-induced atherosclerosis.

• Amyloid-β40 decline is highly involved in Herpud1 deficiency Hcy-induced atherosclerosis process via blocking the JNK/AP1 pathway.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Gao, F., Zhang, J., Ni, T. et al. Herpud1 deficiency could reduce amyloid-β40 expression and thereby suppress homocysteine-induced atherosclerosis by blocking the JNK/AP1 pathway. J Physiol Biochem (2020). https://doi.org/10.1007/s13105-020-00741-5

Download citation

Keywords

  • Homocysteine
  • Atherosclerosis
  • Herpud1 deficiency
  • Amyloid-β40
  • JNK/AP1 pathway