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Glutathione-dependent enzyme activities of peripheral blood mononuclear cells decrease during the winter season compared with the summer in normal-weight and severely obese adolescents

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Abstract

Oxidative stress-related inflammation is known to play a vital role in obesity-associated cardiovascular disease, contributing to the early stages of the pathology as well as during its development. Therefore, it is of great interest to understand how obesity-induced stress modulates antioxidant enzyme activity during puberty. To this end, 27 severely obese adolescents (body mass index > 30, z-score > 3.7) were recruited from a paediatric weight management centre. Eighteen were recruited during the summer and nine in the winter. All underwent a 4-month weight loss programme consisting in diet and physical activity. Twenty normal-weight age-matched adolescents were recruited from the same geographical area to serve as controls. Blood samples were extracted, and antioxidant enzyme activities were determined in peripheral blood mononuclear cells (PBMCs) and erythrocytes. The enzymes studied included catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase. Severely obese adolescents presented lower PBMC-glutathione reductase activity than their corresponding normal-weight counterparts. In addition, glutathione-dependent activities tended to be lower in both groups during the winter compared with summer. These changes coincided with differences in circulating vitamin D levels. Results may suggest that season-dependent factors such as vitamin D could affect glutathione-dependent activities in severely obese as well as in normal-weight adolescents.

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Acknowledgements

VM and ER are members of CIBERobn (Fisiopatología de la Obesidad y la Nutrición CB12/03/30038) Instituto de Salud Carlos III, Spain.

Funding

This work was supported by Generalitat Valenciana under the PROMETEO grant 2016/006 to VM and ER and grants from the French Society of Vascular Medicine to AV and A P-M.

Author information

Authors and Affiliations

  1. Biochemistry and Cell Therapy Unit, Institute of Bioengineering, University Miguel Hernandez, Elche, Alicante, Spain

    David Montero & Nestor Vicente-Salar

  2. LaPEC, Faculty of Sciences, University of Avignon, Avignon, France

    David Montero, Guillaume Walther & Agnes Vinet

  3. Institute of Molecular and Cell Biology, University Miguel Hernandez, Elche, Alicante, Spain

    Maria Herranz & Vicente Micol

  4. CIBERobn (Fisiopatología de la Obesidad y la Nutrición CB12/03/30038), Instituto de Salud Carlos III, Madrid, Spain

    Vicente Micol & Enrique Roche

  5. Vascular Medicine Unit, Nimes University Hospital, Nîmes, France

    Antonia Pérez-Martín

  6. Department of Applied Biology-Nutrition, Alicante Institute for Health and Biomedical Research (ISABIAL-FISABIO Foundation), University Miguel Hernandez, Alicante, Spain

    Enrique Roche

  7. Instituto de Bioingeniería, Universidad Miguel Hernández, Avda de la Universidad, 03202, Elche, Spain

    Enrique Roche

Authors
  1. David Montero
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  2. Nestor Vicente-Salar
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  3. Maria Herranz
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  4. Vicente Micol
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  5. Guillaume Walther
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  6. Antonia Pérez-Martín
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  7. Agnes Vinet
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  8. Enrique Roche
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Corresponding author

Correspondence to Enrique Roche.

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The protocol was in accordance with national legal requirements and the Helsinki Declaration for research on human beings and approved by the Ethics Committee for People Protection of the South-Mediterranean Area-III (France).

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Montero, D., Vicente-Salar, N., Herranz, M. et al. Glutathione-dependent enzyme activities of peripheral blood mononuclear cells decrease during the winter season compared with the summer in normal-weight and severely obese adolescents. J Physiol Biochem 75, 321–327 (2019). https://doi.org/10.1007/s13105-019-00693-5

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  • DOI: https://doi.org/10.1007/s13105-019-00693-5

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