Effect of high chronic intake of sucrose on liver metabolism in aging rats. Modulation by rutin and micronutrients

  • Eva Gatineau
  • Frédéric Capel
  • Dominique Dardevet
  • Jérémie David
  • Corinne Pouyet
  • Sergio Polakof
  • Laurent Mosoni
Original Article


High-sugar intake and senescence share common deleterious effects, in particular in liver, but combination of these two factors was little studied. Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation. Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared. We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters. Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates. Gene expression was little changed by sucrose, with lower levels for LXR-α and LXR-β. Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities. In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals. However, it probably attenuated the positive impact of polyphenol/micronutrients.


Fructose Polyphenols Protein synthesis Lipids Triglycerides 



We thank Christophe Del’homme, Philippe Denis, Philippe Lhoste, and Arlette Cissoire for animal management and Nordine Hafnaoui and Brigitte Laillet for technical assistance.

This work was supported by Institut National de la Recherche Agronomique (INRA), France. Similar results as those of this study were published in a preliminary form in the result section of Gatineau Eva thesis on the following website:


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Copyright information

© University of Navarra 2018

Authors and Affiliations

  1. 1.UNH, Unité de Nutrition Humaine, PFEM, MetaboHUB-ClermontUniversité Clermont Auvergne, INRATheixFrance

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