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CXCL5 regulation of proliferation and migration in human non-small cell lung cancer cells

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Abstract

Epithelial neutrophil-activating peptide-78 (CXCL5), a member of the subgroup of CXC-type chemokine family, is an inflammatory factor involved in the progression of lung cancer, but the underlying mechanism remains unclear. In this study, we investigated the effects of CXCL5 on proliferation and migration in non-small cell lung cancer (NSCLC) using tissue microarrays from NSCLC patients and H460 cells transfected with a CXCL5-interfered lentivirus vector or stimulated with recombinant CXCL5. We observed that the expression of CXCL5 was significantly higher in lung cancer cell lines, and high CXCL5 was associated with high chemokine (C-X-C motif) receptor 2 expression and was significantly associated with poor differentiation. The high expression of CXCL5 was associated with poor NSCLC prognosis and was an independent predictive factor. Furthermore, downregulation of CXCL5 in H460 cells significantly reduced proliferation and migration. Recombinant CXCL5 promoted H460 cell proliferation and movement by activating MAPK/ERK1/2 and PI3K/AKT signaling. Our study elucidates the important role of CXCL5 in the progression and prognosis of NSCLC. These findings suggested that CXCL5 might be a potential biomarker and novel therapeutic target for lung cancer.

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Funding

This study was funded by the National Natural Science Foundation of China (grant number 81372313) and Outstanding Youth Foundation of Zhongshan Hospital (grant number 2017ZSYQ28).

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Author notes

  1. Lin Wang and Lin Shi contributed equally to this work

Authors and Affiliations

  1. Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai, 200032, China

    Lin Wang, Jie Gu, Cheng Zhan, Junjie Xi, Jianyong Ding & Di Ge

  2. Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China

    Lin Shi

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  1. Lin Wang
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  2. Lin Shi
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  3. Jie Gu
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  4. Cheng Zhan
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  7. Di Ge
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Correspondence to Junjie Xi or Jianyong Ding.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Wang, L., Shi, L., Gu, J. et al. CXCL5 regulation of proliferation and migration in human non-small cell lung cancer cells. J Physiol Biochem 74, 313–324 (2018). https://doi.org/10.1007/s13105-018-0619-z

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  • DOI: https://doi.org/10.1007/s13105-018-0619-z

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