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Development of SNP markers associated with growth-related genes of Pelodiscus sinensis

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Abstract

The Chinese soft-shelled turtle (Pelodiscus sinensis) is one of the most important aquatic animals in China. The low growth rate is the main factor which hinders the development of the turtle’s industry. Therefore, it is urgent to development available molecular marker associated with genetic variation in growth for P. sinensis. The aim of this study was to identify and validate SNPs from growth-related gene of P. sinensis. The Illumina Hiseq4000 sequencing was used to develop single nucleotide polymorphism (SNP) markers in P. sinensis. Among them, 38 SNPs from growth-related genes were randomly selected and 24 loci exhibited significant polymorphism in genotyping by ARMS-PCR. The observed and expected heterozygosity ranged from 0.0625 to 0.5208 and 0.0807 to 0.4974, respectively. Significant deviation from HWE was observed for 3 loci. These findings provide molecular basis and candidate markers for P. sinensis breeding.

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Data availability

The sequencing data from this study has been archived at the NCBI Sequence Read Archive database (SRP119729).

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Acknowledgements

This study was supported by scientific research fund project of study on the Chinese soft-shell turtle (Pelodiscus sinensis) (The natural science foundation of Hunan province-2017JJ3134/2016NK2115); Scientific research fund of Hunan province education department-17C0935; The national natural science foundation of China-31672640/31372530).

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Authors and Affiliations

  1. College of Animals Science and Technology, Hunan Agricultural University, Changsha, 410128, People’s Republic of China

    Dan Zeng, Xiao Li, Xiao-qing Wang & Gang Xiong

  2. Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, 415000, People’s Republic of China

    Dan Zeng, Xiao Li, Xiao-qing Wang & Gang Xiong

  3. Hunan Biological Electromechanical Vocational and Technical College, Changsha, 410128, People’s Republic of China

    Gang Xiong

Authors
  1. Dan Zeng
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  2. Xiao Li
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  3. Xiao-qing Wang
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  4. Gang Xiong
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Corresponding authors

Correspondence to Xiao-qing Wang or Gang Xiong.

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Zeng, D., Li, X., Wang, Xq. et al. Development of SNP markers associated with growth-related genes of Pelodiscus sinensis. Conservation Genet Resour 12, 87–92 (2020). https://doi.org/10.1007/s12686-018-1065-5

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  • DOI: https://doi.org/10.1007/s12686-018-1065-5

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