Characterization and phylogenetic analysis of the chloroplast genome of Lupinus westianus, a endemic species to Florida, United States
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The complete chloroplast genome sequence of Lupinus westianus, an endangered and endemic species to Florida, United States, has been assembled from Illumina pair-end sequencing. The chloroplast genome structure of L. westianus is a circular molecule of 154,270 bp in length, with a large single copy (LSC) region of 82,437 bp, a small single copy (SSC) region of 15,853 bp, and a pair of inverted repeats (IRs) regions of 27,990 bp. The total G+C content of chloroplast genome is 36.47%, and while it is 41.56% of IRs, which was higher than LSC and SSC regions. The genome contained 130 genes, including 85 protein coding genes, 37 tRNA genes and 8 rRNA genes, of which 18 were duplicated in the IRs region. The phylogenetic analysis indicated that L. westianus was clustered together with other two genus of Lupinus, L. luteus and L. albus. The chloroplast genome of L. westianus laid a good foundation for genetic resoures conservation. The chloroplast genome reported provided useful genomic resources not only for the exchange of information between different species, but also for the population genetics of L. westianus and its phylogenetic and evolutionary studies.
KeywordsLupinus westianus Endangered species Endemic species Illumina sequencing Chloroplast genome
The study was financially supported by the Key Technology R&D Program of Hunan Province (2016NK2148, 2016TP2007, 2016TP1014), Forestry Science and Technology Project of Hunan Province (XKL201731) and Science and Technology Major project of Hunan province (2017NK1014).
- IUCN (2017) The IUCN Red List of Threatened Species. Version 2017-3. http://www.iucnredlist.org. Accessed 13 Dec 2017