Conservation Genetics Resources

, Volume 10, Issue 2, pp 205–207 | Cite as

Isolation and characterization of 33 EST-SNP markers in Schizothorax prenanti

  • Jian Zhou
  • Bo Zhou
  • Qiang Li
  • Lu Zhang
  • Jun Du
  • Hua Ye
Technical Note


Prenant’s schizothoracin, Schizothorax prenanti (Tchang), is one of the most important cold-water economic fish species in China. In this study, we reported the isolation and characterization of 33 SNP markers among 30 individuals from one cultured population. The observed and expected heterozygosities ranged from 0.0312 to 0.8438 and 0.0312 to 0.5079, respectively. Polymorphic information content ranged from 0.0302 to 0.3747. Significant deviation from HWE was observed for 5 loci and no significant linkage disequilibrium was found between pairs of loci. These EST-SNP markers are potentially useful for the study of population genetics, construction of genetic linkage and QTL maps in S. prenanti.


Schizothorax prenanti Single nucleotide polymorphism (SNPs) Population diversity 



This research was supported by the National Natural Science Foundation of China (31402302), Fundamental Research Funds for the central Universities (XDJK2017B008), and the Youth Foundation of Southwest University Rongchang Campus (20700938).


  1. Ding R (1994) The Fishes of Sichuan. Sichuan Publishing House of Science and Technology, ChengduGoogle Scholar
  2. Jiang LH, Chen YJ, Zhang JS, Zhu AY, Wu CW, Liu LQ, Lin ZH, Dong YH. (2015) Population structure of large yellow croaker (Larimichthys crocea) revealed by single nucleotide polymorphisms. Biochem Syst Ecol 63:136–142CrossRefGoogle Scholar
  3. Kong LF, Bai J, Li Q (2014) Comparative assessment of genomic SSR, EST–SSR and EST–SNP markers for evaluation of the genetic diversity of wild and cultured Pacific oyster, Crassostrea gigas Thunberg. Aquaculture 420–421:85–91CrossRefGoogle Scholar
  4. Liang JJ, Liu Y, Zhang XF, Zhang XY, Yue BS, Song ZB (2011) An observation of the loss of genetic variability in prenant’s schizothoracin, Schizothorax prenanti, inhabiting a plateau lake. Biochem Syst Ecol 39:361–370CrossRefGoogle Scholar
  5. Luo H, Xiao SJ, Ye H, Zhang ZS, Lv CH, Zheng SM, Wang ZY, Wang XQ (2016) Identification of Immune-Related Genes and Development of SSR/SNP Markers from the Spleen Transcriptome of Schizothorax prenanti. PLoS ONE 11:e0152572CrossRefPubMedPubMedCentralGoogle Scholar
  6. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a Lab-oratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  7. Small MP, Rogers Olive SD, Seeb LW (2015) Chum Salmon genetic diversity in the Northeastern Pacific Ocean assessed with single nucleotide polymorphisms (SNPs): applications to fishery mana;gement. N Am J Fish Manag 35:974–987CrossRefGoogle Scholar
  8. Song J, Song Z, Yue B, Zheng W (2006) Assessing genetic diversity of wild populations of Prenant’s schizothoracin, Schizothorax prenanti, using AFLP markers. Environ Biol Fish 77:79–86CrossRefGoogle Scholar
  9. Song Z, Song J, Yue B (2008) Population genetic diversity of Prenant’s schizothoracin, Schizothorax prenanti, inferred from the mitochondrial DNA control region. Environ Biol Fish 81:247–252CrossRefGoogle Scholar
  10. Vignal A, Milan D, SanCristobal M, Eggen A (2002) A review on SNP and other types of molecular markers and their use in animal genetics. Genet Sel Evol 34:275–305CrossRefPubMedPubMedCentralGoogle Scholar
  11. Wang L, Huang SQ, Xia JH, et al (2015) Genome-wide discovery of gene-related SNPs in Barramundi Lates calcarifer. Conserv Genet Resour 7:605–608CrossRefGoogle Scholar
  12. Wu J, Hou F, Liang J, Zhang X, Song Z, Wei Q (2013a) Development of ten microsatellite DNA markers in a hexaploid fish, Schizothorax prenanti (Tchang). Conserv Genet Resour 5:545–547CrossRefGoogle Scholar
  13. Wu J, Hou F, Wang Y, Wu B, Wei Q, Song Z (2013b) Isolation and characterization of twenty tetranucleotide microsatellite DNA makers in a schizothoracin fish, Schizothorax prenanti (Tchang). Conserv Genet Resour 5:891–894CrossRefGoogle Scholar
  14. Yeh FC, Yang R, Boyle TJ, Xiyan JM (2000) POPGENE 32, microsoft windows-based freeware for popllation genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, EdmontonGoogle Scholar
  15. Zhang M, Nie J, Shen Y, Xu X, Dang Y, Wang R, Li J (2015a) Isolation and characterization of 25 novel EST-SNP markers in grass carp (Ctenopharyngodon idella). Conserv Genet Resour 7:819–822CrossRefGoogle Scholar
  16. Zhang X, Gao X, Wang J, Cao W (2015b) Extinction risk and conservation priority analyses for 64 endemic fishes in the Upper Yangtze River, China. Environ Biol Fishes 98:261–272CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Fisheries InstituteSichuan Academy of Agricultural SciencesChengduChina
  2. 2.Fisheries Breeding and Healthy Cultivation Research CentreSouthwest UniversityChongqingChina

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