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The complete mitochondrial genome of Kele pig (Sus scrofa) using next-generation deep sequencing

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Abstract

Kele pig (Sus scrofa), a semi-captive native breed in the Southwest of China, have a range of light to dark brown shades phenotype, just like the coat color of Duroc in some way, and also are well known for their good meat quality. However, Kele pigs decreased dramatically in recent years. The first complete mitochondrial genome of Kele pig was sequenced by next-generation sequencing in order to develop the mitogenome data for genus S. scrofa. The total length of the mitogenome is 16,828 bp, which contains 1 control region, 2 ribosomal RNA genes, 13 protein-coding genes and 22 transfer RNA genes. Phylogenetic analyses based on the complete sequences from several breeds were analyzed by neighbor-joining methods and showed that Kele pigs have closer relationships with domestic pigs in southwest region of China. These results are useful for pig phylogenetic study and evolution study of pig genomes.

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References

  • Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Dong L, Maoliang R, Li Z, Chen B (2016) The complete mitochondrial genome sequence of Meishan pig (Sus Scrofa) and a phylogenetic study. Mitochondrial DNA Part B 1:112–113

    Article  Google Scholar 

  • Grant JR, Stothard P (2008) The CGView Server: a comparative genomics tool for circular genomes. Nucleic Acids Res 36:W181–W184

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Jiang F et al (2016) The first complete mitochondrial genome of Dacus longicornis (Diptera: Tephritidae) using next-generation sequencing and mitochondrial genome phylogeny of Dacini tribe. Sci Rep 6:36426

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kitpipit T, Linacre A (2012) The complete mitochondrial genome analysis of the tiger (Panthera tigris). Mol Biol Rep 39:5745–5754

    Article  PubMed  CAS  Google Scholar 

  • Perna NT, Kocher TD (1995) Patterns of nucleotide composition at fourfold degenerate sites of animal mitochondrial genomes. J Mol Evol 41:353–358

    Article  PubMed  CAS  Google Scholar 

  • Ran M-L, Wang M-f, Yang A-Q, Li Z, Chen B (2016) The complete mitochondrial genome of Congjiang miniature pig (Sus scrofa). Mitochondrial DNA Part A 27:1787–1788

    Article  CAS  Google Scholar 

  • Saccone C, De Giorgi C, Gissi C, Pesole G, Reyes A (1999) Evolutionary genomics in Metazoa: the mitochondrial DNA as a model system. Gene 238:195–209

    Article  PubMed  CAS  Google Scholar 

  • Saccone C, Gissi C, Reyes A, Larizza A, Sbisa E, Pesole G (2002) Mitochondrial DNA in metazoa: degree of freedom in a frozen event. Gene 286:3–12

    Article  PubMed  CAS  Google Scholar 

  • Shen L-Y, Liu C-D, Zhang S-H, Zhu L (2016a) The complete sequence of the mitochondrial genome of Liangshan pig (Sus Scrofa). Mitochondrial DNA Part A 27:4183–4184

    Article  CAS  Google Scholar 

  • Shen L-Y, Zhang S-H, Zhu L (2016b) Complete mitochondrial genome sequence of Chenghua pig (Sus Scrofa) and its phylogenetic analysis. Mitochondrial DNA Part B 1:530–531

    Article  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ursing BM, Arnason U (1998) The complete mitochondrial DNA sequence of the pig (Sus Scrofa). J Mol Evol 47:302–306

    Article  PubMed  CAS  Google Scholar 

  • Wang J et al (2010) Phylogenetic relationships of pig breeds from Shandong province of China and their influence by modern commercial breeds by analysis of mitochondrial DNA sequences. Ital J Anim Sci 9:e48

    Article  Google Scholar 

  • Zhang ZG, Li B, Chen X (1986) Pig breeds in China. Shanghai Scientific and Technical Publisher, Shanghai

    Google Scholar 

Download references

Acknowledgements

This research was supported by grants from Guizhou Provincial Department of Science and Technology (Nos. 20154003 and NY20133072) and Modern Swine Industrial Technology System of Guizhou Province (No. GZCYTX2013).

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Author notes

  1. Ya Tan and Kai-zhi Shi contributed equally to this work.

Authors and Affiliations

  1. Institute of Animal Husbandry and Veterinary, Guizhou Academy of Agricultural Science, Guiyang, 550005, Guizhou, China

    Ya Tan, Kai-zhi Shi, Jing Wang, Chun-lin Du, Chun-ping Zhao, Xiong Zhang & Yi-shun Shang

  2. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China

    Ya Tan & Li Zhu

Authors
  1. Ya Tan
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  2. Kai-zhi Shi
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  3. Jing Wang
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  4. Chun-lin Du
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  5. Chun-ping Zhao
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  6. Xiong Zhang
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  7. Li Zhu
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  8. Yi-shun Shang
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Corresponding authors

Correspondence to Li Zhu or Yi-shun Shang.

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The authors declare that they have no conflict of interest.

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The animal experiment in the study was conducted according to the Chinese Ministry of Science and Technology Guiding Directives for Humane Treatment of Laboratory Animals.

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Tan, Y., Shi, Kz., Wang, J. et al. The complete mitochondrial genome of Kele pig (Sus scrofa) using next-generation deep sequencing. Conservation Genet Resour 10, 195–199 (2018). https://doi.org/10.1007/s12686-017-0797-y

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  • DOI: https://doi.org/10.1007/s12686-017-0797-y

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