Conservation Genetics Resources

, Volume 10, Issue 2, pp 259–268 | Cite as

Barcoding PCR primers detect larval lake sturgeon (Acipenser fulvescens) in diets of piscine predators

  • Justin M. Waraniak
  • Danielle M. Blumstein
  • Kim T. Scribner
Methods and Resources Article


Population levels of recruitment are often affected by high rates of mortality during early life stages. Lake sturgeon (Acipenser fulvescens), a regionally threatened species, experiences high rates of mortality during the larval stage, partially due to predation. The objective of this study was to quantify and compare relative rates of larval sturgeon predation by piscine predators in the upper Black River (Cheboygan County, MI, USA). A molecular barcoding assay was developed using lake sturgeon-specific primers that amplify a region of mitochondrial DNA cytochrome oxidase I as an alternative to morphological analysis of gastrointestinal (GI) contents to quantify the presence or absence of larval fish collected from potential fish predators (353 specimens, 17 potential predator species). The assay was verified to be sturgeon-specific and sufficiently sensitive to amplify the low quantities of degraded DNA in GI samples. Lake sturgeon DNA was identified in 26 of 353 predator diet samples (7.34%) in 9 of 17 potential predator fish species present. There was a significant positive correlation between the numbers of predators that had consumed larval lake sturgeon and the number of samples from a predator species analyzed. No relationship between predation level and habitat type (sand or gravel substrate) was observed, though predator community composition varied between habitats. Genetic assays as described here can be used to investigate predator–prey dynamics affecting species of conservation interest during important life stages that may otherwise be under-represented in diet studies that rely solely on morphological analysis.


Lake sturgeon Predation Larval fish Molecular diet analysis Cytochrome c oxidase mtDNA barcoding 



This project was funded by the Michigan Department of Natural Resources, the Great Lakes Fisheries Trust, and the US Fish and Wildlife Service Coastal Program. Special thanks to the 2015 field crew at the Black River Sturgeon Rearing Facility, Sturgeon For Tomorrow—Black Lake Chapter, Dr. Ed Baker, and Jeanette Kanefsky.

Supplementary material

12686_2017_790_MOESM1_ESM.pdf (79 kb)
Supplementary material 1 (PDF 79 KB)


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Justin M. Waraniak
    • 1
  • Danielle M. Blumstein
    • 2
  • Kim T. Scribner
    • 1
    • 2
  1. 1.Department of Fisheries and WildlifeMichigan State UniversityEast LansingUSA
  2. 2.Department of Integrative BiologyMichigan State UniversityEast LansingUSA

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