Abstract
Manglietia insignis, a rare and endangered evergreen tree endemic to South Asia, is of great importance for landscaping because of its graceful appearance and red lotus-like flowers. In this study, the complete chloroplast (cp) genome of M. insignis was assembled based on the Illumina sequences. The cp genome of M. insignis was 160,117 bp in length and contained a pair of inverted regions (IR, 26,575 bp) which were separated by the small single copy (SSC, 18,660 bp) and the large single copy (LSC, 88,307 bp) regions. It encoded 131 genes including 86 protein-coding genes, 37 tRNA genes, and eight rRNA ribosomal genes. The overall AT content of M. insignis cp genome is 60.8%. The maximum likelihood phylogenetic analysis revealed that M. insignis was closely related to Michelia odora. This result will be helpful for the conservation, taxonomy, and phylogeny programs of the genus Manglietia.
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References
Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for illumina sequence data. Bioinformatics 30:2114–2120
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Guan WL, Kuan DL, Yang WL, Liu ZY (2011) Effects of cutting roots during different periods on transplanting survival in Manglietia insignis. SW China J Agr Sci 2:069
Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780
Khela S (2014) Magnolia insignis. The IUCN red list of threatened species 2014: e.T191875A2011399. http://dx.doi.org/10.2305/IUCN.UK.2014-1.RLTS.T191875A2011399.en. Accessed on 09 April 2017
Kuang DY, Wu H, Wang YL, Gao LM, Zhang SZ, Lu L (2011) Complete chloroplast genome sequence of Magnolia kwangsiensis (Magnoliaceae): implication for DNA barcoding and population genetics. Genome 54:663–673
Li B, Li Y, Cai Q, Lin F, Meng Q, Zheng Y (2016) The complete chloroplast genome of a Tertiary relict species Liriodendron chinense (Magnoliaceae). Conserv Genet Resour 8:279–281
Lohse M, Drechsel O, Kahlau S, Bock R (2013) Organellar Genome DRAW—a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucleic Acids Res 41:W575–W581
Lu Y, Wu Q, Gong X, Zhang Q, Zhang Y (1998) Study on the sexual reproduction and biological characteristics of Manglietia insignis. Guihaia 19:267–271
Pan Y, Gong X, Liang H (2000) Studies on the formation of microspores and development of male gametes in Manglietia insignis. Acta Bot Yunnan 23:85–90
Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313
Wang Y, Li Y, Zhang S, Yu X (2005) The utility of matK gene in the phylogenetic analysis of the genus Magnolia. Acta Phytotaxon Sin 44:135–147
Xia N, Liu Y, Hans PN (2008) Magnoliaceae. In: Wu CY, Raven P (eds) In: Flora of China Editorial Committee (ed) Flora of China, vol 7. Science Press/Missouri Botanical Garden Press, Beijing/St. Louis, pp 51–56
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This study was supported by the China Scholarship Council.
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Zheng, W., Xu, X. The complete chloroplast genome of endangered Manglietia insignis, a rare landscaping tree with red lotus-like flowers. Conservation Genet Resour 10, 27–30 (2018). https://doi.org/10.1007/s12686-017-0754-9
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DOI: https://doi.org/10.1007/s12686-017-0754-9