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Isolation, characterization and cross-species amplification of polymorphic microsatellite markers for Oxytenanthera abyssinica (A. Rich.) Munro (Poaceae)

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Abstract

We developed microsatellite to investigate genetic diversity of Oxytenanthera abyssinica (Bindura bamboo) a bamboo species endemic to Senegal. Using the microsatellite-enriched library method, we identified 14 primer pairs. The primers amplified 10 di-nucleotide repeats and 4 composite motifs, the number of alleles ranged from 5 to 28 with an average of 11.9. The results indicate that these primer pairs offer an appropriate amount of variation to address issues of germplasm characterization, patterns of gene flow and conservation studies. Cross amplification has been tested in other bamboo species.

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Acknowledgments

A.N. was a postdoctoral fellow funded by the Agence Universitaire de la Francophonie (AUF). We greatly appreciate the leadership of Prafrance for allowing us to get the ten samples of bamboo.

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Authors and Affiliations

  1. Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta DIOP, BP 5005, Dakar, Senegal

    Aliou Ndiaye, Maurice Sagna & Yaye Kène Gassama

  2. Cirad, UMR AGAP, 34398, Montpellier Cedex 5, France

    Ronan Rivallan, Thierry Legavre & Ange-Marie Risterucci

  3. INRA, UR 1279 EPGV, CEA Institut de Génomique, Centre National de Génotypage, CP5724, Evry, France

    Dominique Brunel

Authors
  1. Aliou Ndiaye
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  2. Ronan Rivallan
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  3. Thierry Legavre
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  4. Dominique Brunel
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  5. Maurice Sagna
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  6. Yaye Kène Gassama
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  7. Ange-Marie Risterucci
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Correspondence to Aliou Ndiaye.

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Ndiaye, A., Rivallan, R., Legavre, T. et al. Isolation, characterization and cross-species amplification of polymorphic microsatellite markers for Oxytenanthera abyssinica (A. Rich.) Munro (Poaceae). Conservation Genet Resour 5, 799–802 (2013). https://doi.org/10.1007/s12686-013-9911-y

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  • DOI: https://doi.org/10.1007/s12686-013-9911-y

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