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Development and characterization of polymorphic microsatellite marker for Dactylorhiza hatagirea (D. Don) Soo

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Abstract

Dactylorhiza hatagirea (D. Don) Soo is a critically endangered orchid distributed in eastern China and central Asia. In order to investigate its genetic diversity within and between populations and design effective conservation strategies, we developed microsatellite marker for this species in the present study. A total of 14 polymorphic markers were isolated and characterized using 68 individuals from three natural populations. The number of alleles per locus for a single population ranged from 2 to 15, and the polymorphism information content ranged from 0.043 to 0.908. No genotypic linkage disequilibrium within populations was detected. These microsatellite markers are the first characterized for D. hatagirea and they are expected to be useful for population level studies in this species.

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Acknowledgments

This work was supported by grants from the Province Natural Science Foundation of Qinghai (No. 2011-Z-908), PCSIRT and Shanghai Leading Academic Discipline Project (B11).

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Authors and Affiliations

  1. School of Life Sciences, Fudan University, Shanghai, 200433, China

    Pengcheng Lin, Liyan Zeng, Zhen Yang & Yang Zhong

  2. College of Chemistry and Life Sciences, Qinghai University for Nationalities, Xining, 810007, China

    Pengcheng Lin & Ruike Liu

  3. Institute of Biodiversity Science and Geobiology, Tibet University, Lhasa, 850000, China

    Yang Zhong

Authors
  1. Pengcheng Lin
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  2. Liyan Zeng
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  3. Zhen Yang
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  4. Ruike Liu
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  5. Yang Zhong
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Correspondence to Yang Zhong.

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Lin, P., Zeng, L., Yang, Z. et al. Development and characterization of polymorphic microsatellite marker for Dactylorhiza hatagirea (D. Don) Soo. Conservation Genet Resour 6, 29–31 (2014). https://doi.org/10.1007/s12686-013-0053-z

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  • DOI: https://doi.org/10.1007/s12686-013-0053-z

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