Development and cross-amplification of thirty microsatellite loci in five diploid and polyploid Central Asian species of Palearctic green toads (Bufo viridis subgroup)
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We report 30 polymorphic microsatellite markers for five species of Palearctic green toads (Bufo viridis subgroup): 23 in the diploid B. latastii, 19 in diploid B. turanensis, 20 in diploid B. shaartusiensis, 27 in tetraploid B. pewzowi, and 30 in triploid B. baturae. Genetic diversity at these loci, measured for 10–18 individuals per species, ranged from 2 to 19 alleles. These microsatellite loci will be useful for conservation plans (genetic diversity, population structure, evolutionary units), inheritance patterns, and evolution of green toads.
KeywordsBufo viridis subgroup Microsatellite markers Polyploidy Hybrid speciation
We thank Simon Cawsey, Valery Eremchenko, Stefan Michel, and Bakhtiyor Rasulov for help in the field in Kyrgyzstan and Tajikistan, and Françoise Dolivo for help in the laboratory. This work was funded in part by a grant from the Fondation Agassiz of the University of Lausanne (1 July 2010) and a Heisenberg-Fellowship (Sto 493/2-1) and grant STO 493/3-1 of the Deutsche Forschungsgemeinschaft (DFG), all to MS.
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