Development and characterization of microsatellite markers for the endangered Laotian rock-rat (Laonastes aenigmamus) using 454-sequencing technology
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The Laotian rock rat (Laonastesaenigmamus) is the single surviving member of the family Diatomyidae, which has a distribution restricted to the karstic region of Lao-PDR. Here we describe the development of 12 polymorphic microsatellites markers for the endangered Laotian rock rat using 454-sequencing. We successfully tested 12 markers in 30 individuals from 2 populations. Eleven of the 12 loci were polymorphic and the number of alleles detected per locus ranged from 2 to 11. Three of these loci deviated significantly from Hardy–Weinberg equilibrium, which coincides with the detection of possible null alleles. These microsatellite markers are expected to contribute in future research and conservation of L. aenigmamus.
KeywordsLaotian rock rat Laonastes aenigmanus Khanyou Microsatellite 454 sequencing Conservation genetics
- Aplin K, Lunde D (2008) Laonastes aenigmamus. In: IUCN 2009. IUCN red list of threatened speciesGoogle Scholar
- Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices Version 2.9.3. Available from http://www.unil.ch/izea/softwares/fstat.html [updatedfrom Goudet (1995)]
- Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386Google Scholar