Conservation Genetics Resources

, Volume 4, Issue 4, pp 999–1002 | Cite as

Development and characterization of microsatellite markers for the endangered Laotian rock-rat (Laonastes aenigmamus) using 454-sequencing technology

  • Jorge Luis Pino
  • Marina Sofia Ascunce
  • David Reed
  • Jean-Pierre Hugot
Technical Note


The Laotian rock rat (Laonastesaenigmamus) is the single surviving member of the family Diatomyidae, which has a distribution restricted to the karstic region of Lao-PDR. Here we describe the development of 12 polymorphic microsatellites markers for the endangered Laotian rock rat using 454-sequencing. We successfully tested 12 markers in 30 individuals from 2 populations. Eleven of the 12 loci were polymorphic and the number of alleles detected per locus ranged from 2 to 11. Three of these loci deviated significantly from Hardy–Weinberg equilibrium, which coincides with the detection of possible null alleles. These microsatellite markers are expected to contribute in future research and conservation of L. aenigmamus.


Laotian rock rat Laonastes aenigmanus Khanyou Microsatellite 454 sequencing Conservation genetics 


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Jorge Luis Pino
    • 1
  • Marina Sofia Ascunce
    • 1
  • David Reed
    • 1
  • Jean-Pierre Hugot
    • 2
  1. 1.Florida Museum of Natural HistoryGainesvilleUSA
  2. 2.Muséum National d’Histoire Naturelle, Département Systématique et EvolutionUMR CNRS 7205ParisFrance

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