Abstract
Cystoseira amentacea var. stricta (Sargassaceae) is an endemic alga of the Mediterranean Sea. It grows in the upper sublittoral wave-exposed zone and is submitted to various anthropogenic pressures resulting in population fragmentation. In order to assess the genetic diversity and structure of these populations, we developed eight microsatellite markers and validated them on 57 individuals from two populations. With 4–9 alleles per locus, the observed heterozygosity varied between 0.033 and 0.852. We tested cross-amplification of the eight primers on Cystoseira mediterranea, and it was successful for seven of these primers.
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References
Ballesteros E, Torras X, Pinedo S, Garcia M, Mangialajo L, de Torres M (2007) A new methodology based on littoral community cartography for the implementation of the European Water Framework Directive. Mar Pollut Bull 55:172–180
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol 8:92–94
Holleley CE, Geerts PG (2009) Multiplex Manager 1.0: a crossplatform computer program that plans and optimizes multiplex PCR. Biotechniques 46(7):511–517
Mangialajo L, Chiantore M, Susini ML, Meinisz A, Cattaneo-Vietti R, Thibaut T (2012) Zonation patterns and interspecific relationships of fucoids in microtidal environments. J Exp Mar Biol Ecol 412:72–80
Peakall R, Smouse PE (2005) Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295
Raymond M, Rousset F (1995) GENEPOP (version 3.3): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Soltan D, Verlaque M, Boudouresque CF, Francour P (2001) Changes in macroalgal communities in the vicinity of a Mediterranean sewage outfall after the setting up of a treatment plant. Mar Pollut Bull 42:59–70
Thibaut T, Pinedo S, Torras X, Ballesteros E (2005) Long-term decline of the populations of Fucales (Cystoseira spp. and Sargassum spp.) in the Albères coast (France, north-western Mediterranean). Mar Pollut Bull 50:1472–1489
Toonen RJ, Hughes S (2001) Increased throughput for fragment analysis on ABI prism 377 automated sequencer using a membrane comb and STR and software. Biotechniques 31:1320–1324
Van Oosterhout C, Hutchinson WF, Wills P, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellites data. Mol Ecol Notes 4:535–538
Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
Acknowledgments
This research has been funded by the Water Agency Rhône-Méditerranée-Corse and a PhD grant from the Région PACA for Pauline Robvieux. We would like to thank Thamilla Zamoum, Jérôme Catanéo, Caroline Rocher and Didier Aurelle for their advices and technical assistance.
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Robvieux, P., Videment, J., Ribout, C. et al. First characterization of eight polymorphic microsatellites for Cystoseira amentacea var. stricta (Fucales, Sargassaceae). Conservation Genet Resour 4, 923–925 (2012). https://doi.org/10.1007/s12686-012-9674-x
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DOI: https://doi.org/10.1007/s12686-012-9674-x