Conservation Genetics Resources

, Volume 4, Issue 2, pp 299–301 | Cite as

Molecular sexing of tigers, Panthera tigris

  • R. McEwing
  • K. Ouitavon
  • J. J. Rovie-Ryan
  • Wulansari
  • F. T. Sitam
  • R. Ogden
Technical Note

Abstract

We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.

Keywords

Molecular sex identification Tiger Zinc finger protein Forensics Felids 

References

  1. IUCN (2010) IUCN Red list of threatened species. www.iucnredlist.org. Downloaded on 10 June 2011
  2. Linkie M, Martyr DJ, Holden J, Yanuar A, Hartana AT, Sugardjito J, Leader-Williams N (2003) Habitat destruction and poaching threaten the Sumatran tiger in Kerinci Seblat national park. Sumatra Oryx 37(1):41–48Google Scholar
  3. Pilgrim KL, McKelvey KS, Riddle MK, Schwartz MK (2005) Felid sex identification based on non-invasive genetic samples. Mol Ecol 5(1):60–61CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • R. McEwing
    • 1
  • K. Ouitavon
    • 2
  • J. J. Rovie-Ryan
    • 3
  • Wulansari
    • 4
  • F. T. Sitam
    • 3
  • R. Ogden
    • 1
  1. 1.Royal Zoological Society of ScotlandEdinburghScotland, UK
  2. 2.DNP Wildlife Forensic Science Unit, Wildlife Conservation OfficeDepartment of National Parks, Wildlife and Plant ConservationBangkokThailand
  3. 3.Wildlife Genetic Resource Bank (WGRB) LaboratoryDepartment of Wildlife and National ParksKuala LumpurMalaysia
  4. 4.Eijkman Institute for Molecular BiologyJakartaIndonesia

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