Herein we report a label-free amperometric immunosensor based on reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) hybrid material co-modified by gold and iron-oxide magnetic nanoparticles (Au/MNPs-rGO-TEPA) for detection of carcinoembryonic antigen (CEA). The Au/MNPs-rGO-TEPA nanomaterials were prepared and coated on the working electrode as a sensing platform for antibody (anti-CEA) immobilization and signal amplification. This nanomaterial exhibited some excellent functionalities, such as magnetic and enhanced electrical properties due to its large specific surface area and favorable conductivity. Thionine (Thi) molecule was attached onto rGO-TEPA via π-π stacking and was used as an electrochemically active substance. The immunosensor was developed based on the increase in quantity of CEA captured, proportional to the decrease in peak currents of Thi. Under optimal conditions, the Au/MNPs-rGO-TEPA/Thi-based immunosensor showed impressive linear range from 0.1 pg/mL to 150 ng/mL. In addition, the proposed method had excellent linearity, high sensitivity, and relatively good stability, thereby offering a good platform for early clinical analysis.
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We received support from National Natural Science Foundation of Guangxi key research and development program (Guike AB20072003), National Natural Science Foundation of China (61627807 and 81873913), Natural Science Foundation of Guangxi (2018GXNSFDA281044), China Postdoctoral Science Foundation (2019M653315), Guangxi Key Laboratory of Automatic Detecting Technology and Instruments (YQ20108), and Middle-aged and Young Teachers’ Basic Ability Promotion Project of Guangxi (2019KY0207).
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Xiao, H., Wei, S., Chen, Z. et al. Label-Free Electrochemical Immunosensor Based on Gold and Iron-Oxide Nanoparticle Co-modified rGO-TEPA Hybrid for Sensitive Detection of Carcinoembryonic Antigen. Electrocatalysis 11, 513–521 (2020). https://doi.org/10.1007/s12678-020-00604-z
- Electrochemical immunosensor
- Binary nanoparticle-decorated reduced graphene oxide-tetraethylene pentamine
- Carcinoembryonic antigen