Fig. 5 | Hormones and Cancer

Fig. 5

From: Progesterone Receptor Gene Variants in Metastatic Estrogen Receptor Positive Breast Cancer

Fig. 5

Transient expression of Y890C mutation reduces PR transcription in ER+ breast cancer cells. a T47D PR KO cells (ER+PR−) were deprived of steroid hormones for 3 days, transiently transfected with plasmids expressing either WT PR-A, WT PR-B, Y890C PR-A, Y890C PR-B, or empty vector along with reporter genes. Cells were treated with 10 nM R5020 or ethanol for 24 h. Values represent luciferase activity normalized to β–galactosidase activity. *p < 0.05 compared with EtOH for each receptor type. b Reporter gene assay results in T47D PR KO cells normalized to WT PR in the presence of R5020. c R5020 induction of FKBP5 expression obtained from qPCR in ER+PR+ T47D cells transiently transfected with Y890C PR-A, Y890C PR-B, or empty vector expression plasmids. Values were normalized to EtOH control and expressed as fold change relative to empty vector. Values represent the average ± SEM of 5 different experiments. *p < 0.05 compared with empty vector

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