Fig. 4 | Hormones and Cancer

Fig. 4

From: Progesterone Receptor Gene Variants in Metastatic Estrogen Receptor Positive Breast Cancer

Fig. 4

Stable expression of Y890C mutation reduces PR transcriptional function. a MDA-MB-231 breast cancer cells (ER−PR−) stably transfected with either wild-type (WT) PR-A, WT PR-B, Y890C PR-A, or Y890C PR-B expression plasmids were deprived of steroid hormones for 3 days, transfected with reporter plasmids to measure PR transcriptional activation, then treated with 10 nM R5020 for 24 h. Values for luciferase activity normalized to β-galactosidase activity in the presence of R5020 are normalized to WT PR. * p < 0.05 compared with WT for each isoform. b Immunofluorescence of PR protein localization in the stable cell lines (green = Alexa Fluor 488 staining for PR, blue = DAPI nuclear staining). c Protein expression of WT and Y890C PR-A and PR-B in the stable cell lines

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