In this study, greenly synthesized hybrid chitosan silver nanoparticles were prepared and loaded with doxorubicin (Dox). The prepared silver chitosan nanoparticles (Ch-AgNPs) were characterized by transmission electron microscopy, zeta potential, size distribution measurements, UV/VIS absorption spectroscopy, and FTIR spectroscopy. The doxorubicin release profile was evaluated at different pH to investigate the efficacy of Ch-AgNPs-Dox for controlled Dox release. Additionally, the anti-cancer activity of Ch-AgNPs-Dox was assessed against breast cancer cell line in-vitro and Ehrlich tumor in-vivo. TEM analysis indicates the formation of spherical crystalline nanoparticles. Dynamic light scattering measurements indicate that the average diameter of the prepared nanoparticles is 48 ± 13 nm with zeta potential 57.5 ± 4.67 mv. Moreover, light microscopy examinations revealed a high percentage of cell death in Ch-AgNPs-Dox–treated group compared with Ch-AgNPs or free Dox. The current study provides a new approach for preparing hybrid chitosan silver nanoparticles with sustained Dox release for the purpose of cancer treatment. In this context, the promising Ch-AgNPs-Dox showed a pronounced anti-cancer activity against human breast cancer cells (in-vitro) and subcutaneous tumors (in-vivo).
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The author is thankful to Cairo University for offering chemical products and also for providing all the laboratory facilities for this study.
Conflict of interest
The author declares that there is no conflict of interest.
Research involving Animal
The study protocol met the approval conditions of the institutional animal care and use committee (IACUC) with an approval number (CUIF1820), Cairo University.
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Mohamed, N. Synthesis of Hybrid Chitosan Silver Nanoparticles Loaded with Doxorubicin with Promising Anti-cancer Activity. BioNanoSci. (2020). https://doi.org/10.1007/s12668-020-00760-y
- Green synthesis
- Silver nanoparticles
- Drug delivery