Synthesis of Hybrid Chitosan Silver Nanoparticles Loaded with Doxorubicin with Promising Anti-cancer Activity

Abstract

In this study, greenly synthesized hybrid chitosan silver nanoparticles were prepared and loaded with doxorubicin (Dox). The prepared silver chitosan nanoparticles (Ch-AgNPs) were characterized by transmission electron microscopy, zeta potential, size distribution measurements, UV/VIS absorption spectroscopy, and FTIR spectroscopy. The doxorubicin release profile was evaluated at different pH to investigate the efficacy of Ch-AgNPs-Dox for controlled Dox release. Additionally, the anti-cancer activity of Ch-AgNPs-Dox was assessed against breast cancer cell line in-vitro and Ehrlich tumor in-vivo. TEM analysis indicates the formation of spherical crystalline nanoparticles. Dynamic light scattering measurements indicate that the average diameter of the prepared nanoparticles is 48 ± 13 nm with zeta potential 57.5 ± 4.67 mv. Moreover, light microscopy examinations revealed a high percentage of cell death in Ch-AgNPs-Dox–treated group compared with Ch-AgNPs or free Dox. The current study provides a new approach for preparing hybrid chitosan silver nanoparticles with sustained Dox release for the purpose of cancer treatment. In this context, the promising Ch-AgNPs-Dox showed a pronounced anti-cancer activity against human breast cancer cells (in-vitro) and subcutaneous tumors (in-vivo).

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Acknowledgments

The author is thankful to Cairo University for offering chemical products and also for providing all the laboratory facilities for this study.

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Correspondence to Noha Mohamed.

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The study protocol met the approval conditions of the institutional animal care and use committee (IACUC) with an approval number (CUIF1820), Cairo University.

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Mohamed, N. Synthesis of Hybrid Chitosan Silver Nanoparticles Loaded with Doxorubicin with Promising Anti-cancer Activity. BioNanoSci. (2020). https://doi.org/10.1007/s12668-020-00760-y

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Keywords

  • Green synthesis
  • Chitosan
  • Silver nanoparticles
  • Drug delivery
  • Doxorubicin
  • Anti-cancer