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Effects of Gold Nanoparticles with Different Surface Charges on Cellular Internalization and Cytokine Responses in Monocytes

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Abstract

Surface modification of gold nanoparticles (AuNPs) affects the interactions between the AuNPs and biological systems. To use AuNPs in biomedical applications, an investigation of how different surface charges of AuNPs affect the internalization mechanisms and functions of monocytes is needed. In this study, U937 cells were exposed to AuNPs with positive (AuNPs+ve) and negative (AuNPs-ve) surface charges. Cellular responses to the AuNPs, including cell uptake pathways, cell activation and cytokine production, were analysed. The results indicated different responses to AuNPs-ve and AuNPs+ve in outcomes including internalization pathways, cell activation and cytokine production levels. Interestingly, the results suggested that AuNPs+ve tended to enter the cells via macropinocytosis and strongly elicited a cellular response of IL-6 production via NF-kB activation, whereas the AuNPs-ve suppressed IL-6 production. Therefore, these results provide essential information for designing nanoparticles and may contribute to possible future nanomedicine applications, such as drug delivery and vaccination.

The demonstration of AuNPs+ve and AuNPs-ve were taken up through the immune cells by different pathways via phagocytosis/macropinocytosis, clathrin-mediated endocytosis, and caveolae-mediated endocytosis and clathrin-mediated endocytosis, respectively. Then, the cellular activations were occurred and observed by NF-kB activation and cytokine production.

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Funding

The authors would like to acknowledge financial support from the Thailand Research Fund (TRG5880024) and the Mekong Health Science Research Institute (MeHSRI), Khon Kaen University, Thailand.

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Author notes

  1. Sukanya Srijampa and Surachat Buddhisa contributed equally to this work.

Authors and Affiliations

  1. Biosensor Research Group for Non-Communicable Disease and Infectious Disease, Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Sukanya Srijampa & Patcharaporn Tippayawat

  2. Department of Medical Technology, Faculty of Allied Health Sciences, University of Phayao, Phayao, 56000, Thailand

    Surachat Buddhisa

  3. Materials Science and Nanotechnology Program, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Sawinee Ngernpimai

  4. Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Ramkhamhaeng Road, Hua mak, Bangkapi, Bangkok, 10240, Thailand

    Duangkamon Sangiamdee & Apiwat Chompoosor

  5. Department of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Patcharaporn Tippayawat

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  1. Sukanya Srijampa
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Correspondence to Patcharaporn Tippayawat.

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Srijampa, S., Buddhisa, S., Ngernpimai, S. et al. Effects of Gold Nanoparticles with Different Surface Charges on Cellular Internalization and Cytokine Responses in Monocytes. BioNanoSci. 9, 580–586 (2019). https://doi.org/10.1007/s12668-019-00638-8

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