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Bioelectrochemical Systems as Technologies for Studying Drug Interactions Related to Cytochrome P450

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Abstract

The electrocatalytic activity of cytochrome P450 3A4 as the main enzyme of drug metabolism was studied. Cytochrome P450 electrodes allowed to register 10−11–10−12 mol of enzyme/electrode. The study has shown that the developed electrochemical systems based on cytochromes P450 are effective non-invasive models for the analysis of drug–drug interactions at the level of biotransformation of xenobiotics. The effect of clinically significant drug combinations on the activity of cytochrome P450 3A4 was studied. The electroanalytical response of cytochrome P450 3A4 enzyme was interpreted in terms of its impact on drug interference and assessment of heme protein activity. The results can be used for modulation of cytochrome P450 activity in case of comorbid patients with polypharmacy.

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Acknowledgments

The reported study was supported by Russian Fund of Fundamental Research (RFBR), research project No. 18-04-00374.

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Authors and Affiliations

  1. Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow, Russia, 119121

    Victoria V. Shumyantseva, Tatiana V. Bulko, Alexey V. Kuzikov, Rami A. Masamrekh & Alexander I. Archakov

  2. Pirogov Russian National Research Medical University, Ostrovitianov Street, 1, Moscow, Russia, 117997

    Victoria V. Shumyantseva, Alexey V. Kuzikov, Rami A. Masamrekh & Alexander I. Archakov

  3. Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya st, Moscow, Russia, 119991

    Anna A. Makhova & Evgenia V. Shikh

  4. Institute of Bioorganic Chemistry NASB, 5 Academician V.F. Kuprevich Street, Build 2, 220141, Minsk, Belarus

    Tatyana Shkel, Sergey Usanov & Andrei Gilep

Authors
  1. Victoria V. Shumyantseva
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  2. Anna A. Makhova
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  3. Evgenia V. Shikh
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  4. Tatiana V. Bulko
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Correspondence to Victoria V. Shumyantseva.

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Shumyantseva, V.V., Makhova, A.A., Shikh, E.V. et al. Bioelectrochemical Systems as Technologies for Studying Drug Interactions Related to Cytochrome P450. BioNanoSci. 9, 79–86 (2019). https://doi.org/10.1007/s12668-018-0567-7

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