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Changes in HLA-DR Expression on Monocytes and Lymphocytes in Neonatal Sepsis

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Abstract

HLA-DR is a key marker of leukocyte activation, which suppression determines the effectiveness of immune response and prognosis in neonatal sepsis (NS). The aim of this study was to evaluate the HLA-DR expression on lymphocytes and monocytes in the peripheral blood of neonates with sepsis. The study involved 21 neonates with sepsis and 10 healthy neonates born during the study period. Bacteremia was detected in five cases (24%). The most frequent pathogens found were Klebsiella pneumoniae (n = 2) and Staphylococcus aureus (n = 2) followed by Streptococcus agalactae (n = 1). NS was associated with pneumonia (n = 17), meningitis (n = 3), and necrotizing enterocolitis (n = 1). The onset of NS was accompanied with high blood monocyte activity which ranged from 96 to 100%. Blood CD3+ lymphocyte population and CD4+ and CD8+ lymphocyte subset levels were decreased, with their median values being 1.7, 2.2, and 2.4 times lower than those in the control group, respectively (р = 0.005, р = 0.0002, р = 0.003). The HLA-DR expression on CD3+ lymphocytes, as well as on CD4+ and CD8+ lymphocyte subsets, was extremely low ranging from 0.1 to 7% both in the onset of the disease and in a week after admission to a hospital. Absolute counts of activated CD4+ and CD8+ lymphocyte subsets were significantly lower on the disease first days as compared to the control group (р = 0.02 and р = 0.003, respectively). These results demonstrate adaptive immunity to be non-effective in neonates with sepsis. Low expression of HLA-DR on CD3 lymphocytes as well as on CD4 and CD8 lymphocyte subsets is a reason for immunostimulating therapy.

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Funding

This study was supported by the Russian Government Program of Competitive Growth of the Kazan Federal University. Albert A. Rizvanov was supported by the state assignment 20.5175.2017/6.7 of the Ministry of Education and Science of the Russian Federation.

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Authors and Affiliations

  1. Department of Children Infectious Diseases, Kazan State Medical University, Kazan, Russia

    Khalit S. Khaertynov & Vladimir A. Anokhin

  2. Department of Biochemistry, Kazan State Medical University, Kazan, Russia

    Ilshat G. Mustafin

  3. Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia

    Albert A. Rizvanov & Svetlana A. Sofronova

  4. Neonatal Intensive Care Unit, City Children’s Hospital No.1, Kazan, Russia

    Sergey A. Lubin

  5. Kazan State Medical University, Kazan, Russia

    Asiya Kh. Khaertynova

Authors
  1. Khalit S. Khaertynov
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  2. Vladimir A. Anokhin
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  3. Ilshat G. Mustafin
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  4. Albert A. Rizvanov
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  5. Sergey A. Lubin
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  6. Asiya Kh. Khaertynova
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  7. Svetlana A. Sofronova
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Correspondence to Khalit S. Khaertynov.

Ethics declarations

The Ethics Committee of the City Children’s Hospital approved this study, and a written informed consent was obtained from the parents in accordance with the principles authorized under this protocol (the Federal Law N323-FL dated on 21.11. 2011 “On Protection of Health of the Citizens in the Russian Federation”).

Conflict of Interest

The authors declare that they have no conflicts of interest.

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Khaertynov, K.S., Anokhin, V.A., Mustafin, I.G. et al. Changes in HLA-DR Expression on Monocytes and Lymphocytes in Neonatal Sepsis. BioNanoSci. 8, 647–653 (2018). https://doi.org/10.1007/s12668-018-0519-2

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  • DOI: https://doi.org/10.1007/s12668-018-0519-2

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