Nucleotide Polymorphism in the Iron Utilization System Gene isdB NEAT Domain Affects Heme-Binding Ability of IsdB Protein in Various Human Strains of Staphylococcus aureus
Genetic heterogeneity of the isdB gene fragment encoding one of two heme-binding NEAr Transporter (NEAT) domains pfam05031 in different isolates of Staphylococcus aureus from the healthy carriers and the risk group patients (chronic inflammatory infections of the upper respiratory tract and middle ear) was investigated. All S. aureus strains included in the current study differed phenotypically from one another by degree of affinity to polystyrene with immobilized human hemoglobin. The isolates that displayed low affinity to hemoglobin were found to have more mutations within the isdB gene fragment which encodes one of the IsdB protein domains containing heme-binding sites.
KeywordsStaphylococcus aureus isdB gene Affinity Hemoglobin Inflammatory upper respiratory tract infections
We thank Elena I. Shagimardanova, PhD, Senior Researcher at the Institute of Fundamental Medicine and Biology, KFU, for providing valuable help with sequencing amplified DNA fragments of the isdB gene.
This research was supported by the Federal Service for Supervision of Consumer Rights Protection and Human Welfare research project No. 01201154371 “Factors of virulence of opportunistic bacteria and various microbial human ecosystems in the pathogenesis of infectious and allergic diseases in children and adults.” This work was funded by the grant of the Russian Government to support the Program of Competitive Growth of the Kazan Federal University among the World’s Leading Academic Centers.
Compliance with Ethical Standards
This research was conducted in accordance with the Declaration of Helsinki. An Informed Consent was obtained from all participants, and the study protocol was approved by the Local Ethics Committee at the Kazan Scientific Research Institute of Epidemiology and Microbiology (2010). All data were managed anonymously.
Conflict of Interest
The authors declare that they have no conflicts of interest.
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