Nucleotide Polymorphism in the Iron Utilization System Gene isdB NEAT Domain Affects Heme-Binding Ability of IsdB Protein in Various Human Strains of Staphylococcus aureus
- 18 Downloads
Genetic heterogeneity of the isdB gene fragment encoding one of two heme-binding NEAr Transporter (NEAT) domains pfam05031 in different isolates of Staphylococcus aureus from the healthy carriers and the risk group patients (chronic inflammatory infections of the upper respiratory tract and middle ear) was investigated. All S. aureus strains included in the current study differed phenotypically from one another by degree of affinity to polystyrene with immobilized human hemoglobin. The isolates that displayed low affinity to hemoglobin were found to have more mutations within the isdB gene fragment which encodes one of the IsdB protein domains containing heme-binding sites.
KeywordsStaphylococcus aureus isdB gene Affinity Hemoglobin Inflammatory upper respiratory tract infections
We thank Elena I. Shagimardanova, PhD, Senior Researcher at the Institute of Fundamental Medicine and Biology, KFU, for providing valuable help with sequencing amplified DNA fragments of the isdB gene.
Compliance with Ethical Standards
This research was conducted in accordance with the Declaration of Helsinki. An Informed Consent was obtained from all participants, and the study protocol was approved by the Local Ethics Committee at the Kazan Scientific Research Institute of Epidemiology and Microbiology (2010). All data were managed anonymously.
Conflict of Interest
The authors declare that they have no conflicts of interest.
- 3.Sæderup, K. L., Stødkilde, K., Graversen, J. H., Dickson, C. F., Etzerodt, A., Hansen, S. W., Fago, A., Gell, D., Andersen, C. B., & Moestrup, S. K. (2016). The Staphylococcus aureus protein IsdH inhibits host hemoglobin scavenging to promote heme acquisition by the pathogen. The Journal of Biological Chemistry, 291(46), 23989–23998.CrossRefGoogle Scholar
- 10.Tyurin, Y. A., Fassakhov, R. S., Mustafin, I. G. (2010). A method for determining affinity of Staphylococcus spp to hemoproteins. Russian Federation Patent No. 2393229. Register of Inventions No. 18, published on June 27, 2010 (in Russian).Google Scholar
- 11.Nei, M., & Miller, J. С. (1990). A simple method for estimating average number of nucleotide substitutions within and between populations from restriction data. Genetics, 125, 873–879.Google Scholar