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Intravenous Transplantation of Human Umbilical Cord Blood Mononuclear Cells Overexpressing Nerve Growth Factor Improves Spatial Memory in APP/PS1 Transgenic Mice with a Model of Alzheimer’s Disease

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Abstract

Alzheimer’s disease is a progressive incurable neurodegenerative disease manifested by dementia and other cognitive disorders. Gene-cell therapy is one of the most promising trends in the development of treatment for Alzheimer’s disease. The study was aimed to evaluate the therapeutic potential of intravenous transplantation of human umbilical cord blood mononuclear cells (UCBMCs) transduced with adenoviral vectors overexpressing nerve growth factor (NGF) for the treatment of Alzheimer’s disease in an APP/PS1 transgenic mice model. The transplantation of NGF-expressing UCBMCs was found to improve spatial memory and decrease anxiety in APP/PS1 mice. Grafted cells and their expression of NGF were detected in the cortex and hippocampus of transgenic mice in the period up to 90 days after transplantation. Thus, gene-cell therapy based on the use of NGF-overexpressing UCBMCs is a promising approach for the development of Alzheimer’s disease treatments.

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Acknowledgements

The study was supported by the Scholarship of the President of the Russian Federation for young researchers and scientists (СП-255.2016.4), RFFR grant no. 17-04-02175А. Some aspects of methodology for the development of gene-cell approaches to the treatment of neurodegenerative disorders were implemented with the support by the RSF grant no. 14-15-00847-П. Kazan Federal University facilities were supported by the Russian Government Program of Competitive Growth. Albert A. Rizvanov was personally supported by the state assignment 20.5175.2017/6.7 of the Ministry of Education and Science of the Russian Federation (“Leading Scientist”).

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Authors and Affiliations

  1. Kazan State Medical University, ul. Butlerova, 49, Kazan, 420012, Russia

    M. A. Mukhamedyarov, A. V. Leushina, A. E. Tikhonova, E. O. Petukhova, R. Ben Taleb, Y. O. Mukhamedshina, A. L. Zefirov & R. R. Islamov

  2. Kazan (Volga Region) Federal University, Kazan, Russia

    A. E. Tikhonova, E. E. Garanina, R. Ben Taleb, M. S. Kaligin, Y. O. Mukhamedshina & A. A. Rizvanov

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  1. M. A. Mukhamedyarov
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  2. A. V. Leushina
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  4. E. O. Petukhova
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Correspondence to M. A. Mukhamedyarov.

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Mukhamedyarov, M.A., Leushina, A.V., Tikhonova, A.E. et al. Intravenous Transplantation of Human Umbilical Cord Blood Mononuclear Cells Overexpressing Nerve Growth Factor Improves Spatial Memory in APP/PS1 Transgenic Mice with a Model of Alzheimer’s Disease. BioNanoSci. 8, 473–480 (2018). https://doi.org/10.1007/s12668-017-0497-9

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