, Volume 8, Issue 1, pp 454–458 | Cite as

Capabilities of Molecular-Based Allergen Diagnostics in Atopic Diseases

  • Irina Reshetnikova
  • Svetlana Khaiboullina
  • Elena Agafonova


One hundred thirty-one assays were performed to determine the level of specific IgE to major molecular components of the birch pollen—rBet v 1, timothy grass pollen—rPhl p1, rPhl p 5b, and house dust mites—nDer p1, rDer p2 as well as to the minor components of birch pollen—rBet v 2, rBet v 4, timothy grass pollen—rPhl p7, rPhl p 12, and house dust mites rDer p10 in patients with asthma (N = 66) and allergic rhinitis (N = 65). It is well established that sensitization to major and minor allergens facilitates the selection of an allergen for the allergen-specific immunotherapy (ASIT). According to the concept of molecular-based allergen diagnostics, the ASIT efficacy is expected to be high when IgЕ to major components are present and antibodies to minor components are absent. When elevated IgЕ levels to major and minor ASIT proteins are present, a moderate ASIT efficacy is expected.


Molecular-based allergen diagnostics Major allergen Minor allergen Allergen-specific immunotherapy 



The work was partially supported by the Russian Government Program of Competitive Growth of the Kazan Federal University.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.


  1. 1.
    Canonica, G. W., Ansotegui, I. J., Pawankar, R., et al. (2013). A WAO–ARIA–GA2LEN consensus document on molecular-based allergy diagnostics. World Allergy Organization Journal, 6, 17.CrossRefGoogle Scholar
  2. 2.
    Canonica, G. W., Ansotegui, I. J., Pawankar, R., et al. (2015). Document de consensus WAO–ARIA–GA2LEN sur le diagnostic allergologique moléculaire, Revue Française. Allergologie, 55, 2–83.Google Scholar
  3. 3.
    Valenta, R., Lidholm, J., Niederberger, V., et al. (1999). The recombinant allergen-based concept of component-resoved diagstics and immunotherapy (CRD and CRIT). Clinical and Experimental Allergy, 29, 211–241.CrossRefGoogle Scholar
  4. 4.
    Hauser, M., Roulias, A., Ferreira, F., et al. (2010). Panallergens and their impact on allergic patient. Allergy, Asthma and Clinical Immunology, 6, 1.CrossRefGoogle Scholar
  5. 5.
    Ciprandi, G., Alesina, R., & Ariano, R. (2008). Chracteristics of patients with allergic polysensitization: the POLISMAIL study. European Annals of Allergy and Clinical Immunology, 40, 77–83.Google Scholar
  6. 6.
    Sastre, J. (2010). Molecular diagnosis in allergy. Clinical and Experimental Allergy, 22, 454–461.Google Scholar
  7. 7.
    Jutel, М., & Akdis, C. A. (2011). Immunological mechanisms of allergan-spesific immunological mechanisms of allergen-specific immunonotherapy. Allergy, 66, 725–732.CrossRefGoogle Scholar
  8. 8.
    Cox, L., Nelson, H., Lockey, R., et al. (2009). Allergen immunotherapy practice patterns in the United States and Europe. Annals of Allergy, Asthma & Immunology, 103, 451–459.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Irina Reshetnikova
    • 1
    • 2
  • Svetlana Khaiboullina
    • 2
    • 3
  • Elena Agafonova
    • 1
    • 4
  1. 1.Kazan Scientific-Research Institute of Epidemiology and MicrobiologyKazanRussia
  2. 2.Kazan Federal UniversityKazanRussia
  3. 3.University of NevadaRenoUSA
  4. 4.Kazan State Medical UniversityKazanRussia

Personalised recommendations