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BioNanoScience

, Volume 8, Issue 1, pp 249–253 | Cite as

Biosynthesis of MgO Nanoparticles Using Lactobacillus Sp. and its Activity Against Human Leukemia Cell Lines HL-60

  • V. Mohanasrinivasan
  • C. Subathra Devi
  • Avani Mehra
  • Suman Prakash
  • Aditi Agarwal
  • E. Selvarajan
  • S. Jemimah Naine
Article
  • 66 Downloads

Abstract

The present study reports a low-cost, eco-friendly, and reproducible microbes Lactobacillus sp. mediated biosynthesis magnesium oxide nanoparticles. The nanoparticles were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and high-resolution transmission electron microscopy (HRTEM). The morphological characteristics were found to be spherical, oval in shape, individual nanoparticles as well as a few aggregates. The XRD shows the crystallographic plane indicating that nanoparticles structure dominantly corresponds crystalline. The biosynthesized magnesium oxide nanoparticles showed corresponding functional peaks. The cytotoxic effects of the magnesium oxide nanoparticles could significantly inhibited HL-60cancer cell lines proliferation in a time and concentration-dependent manner by MTT assay. L. sporogenes mediated magnesium oxide nanoparticles had potential to inhibit the cancerous cells by 60% while L. plantarum mediated nanoparticles found to inhibit the growth by 50%. The biosynthesis of nanoparticles has been proposed as an environmental friendly and cost effective alternative to chemical and physical methods. Hence, this report added the value for the application of magnesium oxide nanoparticles in biomedical and nanotechnology applications with the absence of adverse side effects from nonpathogenic, mesophilic Lactobacillus plantarum and Lactobacillus sporogenes for the nanoparticles.

Keywords

Lactobacillus sp. MgO nanoparticles Anti-cancer activity 

Notes

Acknowledgements

We are greatly indebted to Vellore Institute of Technology for the constant encouragement, help, and support for extending necessary facilities.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interests.

References

  1. 1.
    Deshpande, R., Bhat, R., Ganachari, S., Bedre, D., Vasanth, H., Manjunath, S., Bhagawanraju, M., & Venkataraman, A. (2011). Anti-cancer studies of noble metal nanoparticles synthesized using different plant extracts. Cancer Nanotechnology, 11, 57–65.Google Scholar
  2. 2.
    Liu, Z., Kiessling, F., & Gatjens, J. (2010). Advanced nanomaterials in multimodal imaging: design, functionalization, and biomedical applications. Journal of Nano matters, 10, 155–170.Google Scholar
  3. 3.
    Jin, T., & He, Y. (2011). Antibacterial activities of magnesium oxide (MgO) nanoparticles against foodborne pathogens. Journal of Nano Research, 13, 6877–6885.CrossRefGoogle Scholar
  4. 4.
    Tang, Z. X., & Lv, B. F. (2014). MgO nanoparticles as antibacterial agent: preparation and activity. Brazilian Journal of Chemical Engineering, 31, 591–601.CrossRefGoogle Scholar
  5. 5.
    Pal, S., Tak, Y. K., & Song, J. M. (2007). Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle: a study of the gram-negative bacterium Escherichia coli. Applied Environtmental Microbiology, 73, 1712–1720.CrossRefGoogle Scholar
  6. 6.
    Prasad, K., & Jha, A. K. (2009). ZnO nanoparticles: synthesis and adsorption study. Nature Science, 1, 129–135.CrossRefGoogle Scholar
  7. 7.
    Mishra, M., Paliwal, J. S, Singh, S. K, Selvarajan, E., Subathradevi, C., & Mohanasrinivasan, V. (2013). Studies on the inhibitory activity of biologically synthesized and characterized zinc oxide nanoparticles using Lactobacillus sporogenes against Staphylococcus aureus. Journal of Pure and Applied Microbiology, 7(2), 1–6.Google Scholar
  8. 8.
    Selvarajan, E., & Mohanasrinivasan, V. (2013). Biosynthesis and characterization of ZnO nanoparticles using Lactobacillus plantarum VITES07. Materials Letters, 112(0), 180–182.CrossRefGoogle Scholar
  9. 9.
    Sundarajan, M., Suresh, J., & Gandhi, R. (2012). A comparative study on antibacterial properties of MgO nanoparticles prepared under different calcinations temperatures, digest. Journal of Nanomaters and. Bioscience, 7, 983–989.Google Scholar
  10. 10.
    Kang, S., Bader, A. J., & Vogt, P. K. (2007). Phosphatidylinositol 3-kinase mutations identified in human cancer are oncogenic. Proceedings of the National Academy of Sciences, 102, 802–807.CrossRefGoogle Scholar
  11. 11.
    Cao, G. (2004). Nanostructures and nanomaterials: synthesis, properties and applications. London: Imperial College Press.CrossRefGoogle Scholar
  12. 12.
    Gaurav, S., Rajgovind, S., & Nakuleshwar, D. J. (2016). Phytoassisted synthesis of magnesium oxide nanoparticles with Swertia chirayaita. Journal of Taibah University for Science.  https://doi.org/10.1016/j.jtusci.2016.09.004.
  13. 13.
    Bhattacharya, R. (2008). Mukherjee. Biological properties of naked metal nanoparticles. Advance Drug Delivery Reviews, 60, 1289–1306.CrossRefGoogle Scholar
  14. 14.
    Krishnamoorthy, K., Moon, J. Y., Hyun, H. B., Cho, S. K., & Kim, S. J. (2012). Mechanistic investigation on the toxicity of MgO nanoparticles towards the cancer cells. Journal of Materials Chemistry, 22, 24610–24617.CrossRefGoogle Scholar
  15. 15.
    Manoj, K., Patel, M. D., Zafaryab, M., Rizvi, M. A., Agrawal, V. V., Ansari, Z. A., Malhotra, B. D., & Ansari, S. J. (2013). Antibacterial and cytotoxic effect of magnesium oxide nanoparticles on bacterial and human cells. Journal of nanoengineering and nanomanufacturing, 3, 162–166.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • V. Mohanasrinivasan
    • 1
  • C. Subathra Devi
    • 1
  • Avani Mehra
    • 1
  • Suman Prakash
    • 1
  • Aditi Agarwal
    • 1
  • E. Selvarajan
    • 1
    • 2
  • S. Jemimah Naine
    • 1
  1. 1.School of Biosciences and TechnologyVIT UniversityVelloreIndia
  2. 2.Department of Genetic Engineering, School of BioengineeringSRM UniversityKattankulathurIndia

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