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Indication and Identification of Bacillus anthracis Isolates from the Middle Volga Region by Multi-Primer PCR

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Abstract

The article describes the results of improved preparation of various object sampling for indication, identification and genotyping of Bacillus anthracis isolates with molecular genetic methods based on multilocus sequence typing using specific primers. Multi-primer PCR used enabled the detection and differentiation of B. anthracis isolates. The used primers allowed to differentiate isolates by their belonging to the genus Bacillus and B. anthracis species by the рХО1 plasmid presence. The detection of the рХ02 plasmid encoding for capsule formation indicates pathogenicity of the isolates studied. Using multi-primer PCR allowed to determine the epizootic situation of anthrax in the Middle Volga Region and to establish that two genotypic groups of the pathogen B. anthracis circulate in the region.

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This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Institute of Fundamental Medicine and Biology, Kazan Federal University, 18 Kremlyovskaya St., Kazan, Tatarstan, Russian Federation, 420008

    Natalya M. Aleksandrova & Inna A. Rogozhina

  2. Federal Center for Toxicological, Radiation and Biological Safety, Nauchniy Gorodok-2, Kazan, Tatarstan, Russian Federation, 420075

    Natalya M. Aleksandrova, Tagir Kh. Faizov, Nail I. Khammadov & Eduard A. Shuralev

  3. Institute of Environmental Sciences, Kazan Federal University, 18 Kremlyovskaya St., Kazan, Tatarstan, Russian Federation, 420008

    Anna V. Vasileva & Eduard A. Shuralev

  4. Kazan State Medical Academy – Branch Campus of the FSBEI FPE RMACPE MOH Russia, 36 Butlerova St., Kazan, Tatarstan, Russian Federation, 420012

    Eduard A. Shuralev

Authors
  1. Natalya M. Aleksandrova
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  2. Tagir Kh. Faizov
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  3. Anna V. Vasileva
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  4. Inna A. Rogozhina
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  5. Nail I. Khammadov
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  6. Eduard A. Shuralev
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Correspondence to Natalya M. Aleksandrova.

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Aleksandrova, N.M., Faizov, T.K., Vasileva, A.V. et al. Indication and Identification of Bacillus anthracis Isolates from the Middle Volga Region by Multi-Primer PCR. BioNanoSci. 8, 434–440 (2018). https://doi.org/10.1007/s12668-017-0477-0

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  • DOI: https://doi.org/10.1007/s12668-017-0477-0

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