, Volume 8, Issue 1, pp 434–440 | Cite as

Indication and Identification of Bacillus anthracis Isolates from the Middle Volga Region by Multi-Primer PCR

  • Natalya M. Aleksandrova
  • Tagir Kh. Faizov
  • Anna V. Vasileva
  • Inna A. Rogozhina
  • Nail I. Khammadov
  • Eduard A. Shuralev


The article describes the results of improved preparation of various object sampling for indication, identification and genotyping of Bacillus anthracis isolates with molecular genetic methods based on multilocus sequence typing using specific primers. Multi-primer PCR used enabled the detection and differentiation of B. anthracis isolates. The used primers allowed to differentiate isolates by their belonging to the genus Bacillus and B. anthracis species by the рХО1 plasmid presence. The detection of the рХ02 plasmid encoding for capsule formation indicates pathogenicity of the isolates studied. Using multi-primer PCR allowed to determine the epizootic situation of anthrax in the Middle Volga Region and to establish that two genotypic groups of the pathogen B. anthracis circulate in the region.


Bacillus anthracis Anthrax Genotyping Multi-primer PCR Russia 



This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Compliance with Ethical Standards

Animals were not used in this study.

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Natalya M. Aleksandrova
    • 1
    • 2
  • Tagir Kh. Faizov
    • 2
  • Anna V. Vasileva
    • 3
  • Inna A. Rogozhina
    • 1
  • Nail I. Khammadov
    • 2
  • Eduard A. Shuralev
    • 2
    • 3
    • 4
  1. 1.Institute of Fundamental Medicine and BiologyKazan Federal UniversityKazanRussian Federation
  2. 2.Federal Center for Toxicological, Radiation and Biological SafetyKazanRussian Federation
  3. 3.Institute of Environmental SciencesKazan Federal UniversityKazanRussian Federation
  4. 4.Kazan State Medical Academy – Branch Campus of the FSBEI FPE RMACPE MOH RussiaKazanRussian Federation

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