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BioNanoScience

, Volume 8, Issue 1, pp 434–440 | Cite as

Indication and Identification of Bacillus anthracis Isolates from the Middle Volga Region by Multi-Primer PCR

  • Natalya M. Aleksandrova
  • Tagir Kh. Faizov
  • Anna V. Vasileva
  • Inna A. Rogozhina
  • Nail I. Khammadov
  • Eduard A. Shuralev
Article
  • 57 Downloads

Abstract

The article describes the results of improved preparation of various object sampling for indication, identification and genotyping of Bacillus anthracis isolates with molecular genetic methods based on multilocus sequence typing using specific primers. Multi-primer PCR used enabled the detection and differentiation of B. anthracis isolates. The used primers allowed to differentiate isolates by their belonging to the genus Bacillus and B. anthracis species by the рХО1 plasmid presence. The detection of the рХ02 plasmid encoding for capsule formation indicates pathogenicity of the isolates studied. Using multi-primer PCR allowed to determine the epizootic situation of anthrax in the Middle Volga Region and to establish that two genotypic groups of the pathogen B. anthracis circulate in the region.

Keywords

Bacillus anthracis Anthrax Genotyping Multi-primer PCR Russia 

Notes

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Compliance with Ethical Standards

Animals were not used in this study.

Conflict of Interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Huang, Y., Du, Y., Wang, Y., Wang, N., Bai, J., Chen, H., He, H., Xu, J., Wu, Y., Luo, Y., Li, X., & Liang, G. (2016). An outbreak of cutaneous anthrax in Yunnan, China. Emerging Microbes and Infections, 5, e64.  https://doi.org/10.1038/emi.2016.65.CrossRefGoogle Scholar
  2. 2.
    Mondal, T. K., Ghosh, S., Dasgupta, S., & Sarkar, A. P. (2015). Suspected anthrax outbreak: Investigation in a rural block of west Bengal and public health response. Indian Journal of Public Health, 59(4), 302–305.  https://doi.org/10.4103/0019-557X.169662.CrossRefGoogle Scholar
  3. 3.
    Siddiqui, M. A., Khan, M. A., Ahmed, S. S., Anwar, K. S., Akhtaruzzaman, S. M., & Salam, M. A. (2012). Recent outbreak of cutaneous anthrax in Bangladesh: Clinico-demographic profile and treatment outcome of cases attended at Rajshahi medical college hospital. BMC Research Notes, 5, 464.  https://doi.org/10.1186/1756-0500-5-464.CrossRefGoogle Scholar
  4. 4.
    Thapa, N. K., Tenzin, W. K., Dorji, T., Migma, D. J., Marston, C. K., & Hoffmaster, A. R. (2014). Investigation and control of anthrax outbreak at the human-animal interface, Bhutan, 2010. Emerging Infectious Diseases, 20(9), 1524–1526.  https://doi.org/10.3201/eid2009.140181.CrossRefGoogle Scholar
  5. 5.
    Kracalik, I., Malania, L., Tsertsvadze, N., Manvelyan, J., Bakanidze, L., Imnadze, P., Tsanava, S., & Blackburn, J. K. (2014). Human cutaneous anthrax, Georgia 2010-2012. Emerging Infectious Diseases, 20(2), 261–264.  https://doi.org/10.3201/eid2002.130522.CrossRefGoogle Scholar
  6. 6.
    Antwerpen, M., Elschner, M., Gaede, W., Schliephake, A., Grass, G., Tomaso, H. (2016) Genome sequence of Bacillus anthracis strain Stendal, isolated from an anthrax outbreak in cattle in Germany. Genome Announcements, 4(2), e00219-16.  https://doi.org/10.1128/genomeA.00219-16.
  7. 7.
    Nsoh, A. E., Kenu, E., Forson, E. K., Afari, E., Sackey, S., Nyarko, K. M., & Yebuah, N. (2016). Mapping as a tool for predicting the risk of anthrax outbreaks in northern region of Ghana. The Pan African Medical Journal, 25(Suppl 1), 14.  10.11604/pamj.supp.2016.25.1.6205.Google Scholar
  8. 8.
    Lehman, M. W., Craig, A. S., Malama, C., Kapina-Kany'anga, M., Malenga, P., MunsakaF, M. S., Shadomy, S., & Marx, M. A. (2017). Role of food insecurity in outbreak of anthrax infections among humans and hippopotamuses living in a game reserve area, rural Zambia. Emerging Infectious Diseases, 23(9), 1471–1477.  https://doi.org/10.3201/eid2309.161597.CrossRefGoogle Scholar
  9. 9.
    Ozanich, R. M., Colburn, H. A., Victry, K. D., Bartholomew, R. A., Arce, J. S., Heredia-Langner, A., Jarman, K., Kreuzer, H. W., & Bruckner-Lea, C. J. (2017). Evaluation of PCR systems for field screening of bacillus anthracis. Health Security, 15(1), 70–80.  https://doi.org/10.1089/hs.2016.0043.CrossRefGoogle Scholar
  10. 10.
    Terry, C., Jiang, S., Radford, D. S., Wan, Q., Tzokov, S., Moir, A., & Bullough, P. A. (2017). Molecular tiling on the surface of a bacterial spore – The exosporium of the bacillus anthracis/cereus/thuringiensis group. Molecular Microbiology, 104(4), 539–552.  https://doi.org/10.1111/mmi.13650.CrossRefGoogle Scholar
  11. 11.
    Makino, S., Uchida, I., Terakado, N., Sasakawa, C., & Yoshikawa, M. (1989). Molecular characterization and protein analysis of the cap region, which is essential for encapsulation in bacillus anthracis. Journal of Bacteriology, 171(2), 722–730.CrossRefGoogle Scholar
  12. 12.
    Koehler, T. M. (2002). Bacillus anthracis genetics and virulence gene regulation. Current Topics in Microbiology and Immunology, 271, 143–164 Review.Google Scholar
  13. 13.
    Siqueira, FM., Cibulski, SP., Mayer, FQ., Driemeier, D., Pavarini, SP., Vargas, APC. (2017) Genome sequencing of two bacillus anthracis strains: A virulent strain and a vaccinal strain. Brazilian Journal of Microbiology.  https://doi.org/10.1016/j.bjm.2017.04.007.
  14. 14.
    Keefer, A. B., Asare, E. K., Pomerantsev, A. P., Moayeri, M., Martens, C., Porcella, S. F., Gottesman, S., Leppla, S. H., & Vrentas, C. E. (2017). In vivo characterization of an Hfq protein encoded by the bacillus anthracis virulence plasmid pXO1. BMC Microbiology, 17(1), 63.  https://doi.org/10.1186/s12866-017-0973-y.CrossRefGoogle Scholar
  15. 15.
    Liang, X., Zhu, J., Zhao, Z., Zheng, F., Zhang, H., Wei, J., Ji, Y., & Ji, Y. (2017). Thepag gene of pXO1 is involved in capsule biosynthesis of bacillus anthracis Pasteur II strain. Frontiers in Cellular and Infection Microbiology, 7, 203.  https://doi.org/10.3389/fcimb.2017.00203.CrossRefGoogle Scholar
  16. 16.
    Pomerantsev, A. P., McCall, R. M., Chahoud, M., Hepler, N. K., Fattah, R., & Leppla, S. H. (2017). Genome engineering in bacillus anthracis using tyrosine site-specific recombinases. PLoS One, 12(8), e0183346.  https://doi.org/10.1371/journal.pone.0183346.CrossRefGoogle Scholar
  17. 17.
    Antwerpen, MH., Sahl, JW., Birdsell, D., Pearson, T., Pearce, MJ., Redmond, C., Meyer, H., Keim, PS. (2017) Unexpected relations of historical Anthrax strain. MBio, 8(2), e00440-17.  https://doi.org/10.1128/mBio.00440-17.
  18. 18.
    Daas, MS., Rosana, ARR., Acedo, JZ., Nateche, F., Kebbouche-Gana, S., Vederas, JC., Case, RJ. (2017) Draft genome sequences of Bacillus cereus E41 and Bacillus anthracis F34 isolated from Algerian Salt Lakes. Genome Announcements, 5(20), e00383-17.  https://doi.org/10.1128/genomeA.00383-17.
  19. 19.
    Khmaladze, E., Dzavashvili, G., Chanturia, G., Nikolich, MP., Chain, PSG., Johnson, SL., Imnadze, P. (2017) Ten genome sequences of human and livestock isolates of Bacillus anthracis from the country of Georgia. Genome Announcements, 5(19), e00256-17.  https://doi.org/10.1128/genomeA.00256-17.
  20. 20.
    Antwerpen M, Wölfel R, Grass G (2017) Genome sequence of historical Bacillus anthracis strain Tyrol 4675 isolated from a bovine Anthrax case in Austria. Genome Announcements, 5(10), e00002-17.  https://doi.org/10.1128/genomeA.00002-17.
  21. 21.
    Riazanova, A. G., Eremenko, E. I., Tsygankova, O. I., & Tsygankova, E. A. (2009). Improvement of methods for identification of atypical anthrax strains and their differentiation from closely related bacilli. Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii, 3, 76–80.Google Scholar
  22. 22.
    Dubiley, S., Kirillov, E., & Mirzabekov, A. (1999). Polymorphism analysis and gene detection by minisequencing on an array of gel-immobilized primers. Nucleic Acids Research, 27(18), e19.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Natalya M. Aleksandrova
    • 1
    • 2
  • Tagir Kh. Faizov
    • 2
  • Anna V. Vasileva
    • 3
  • Inna A. Rogozhina
    • 1
  • Nail I. Khammadov
    • 2
  • Eduard A. Shuralev
    • 2
    • 3
    • 4
  1. 1.Institute of Fundamental Medicine and BiologyKazan Federal UniversityKazanRussian Federation
  2. 2.Federal Center for Toxicological, Radiation and Biological SafetyKazanRussian Federation
  3. 3.Institute of Environmental SciencesKazan Federal UniversityKazanRussian Federation
  4. 4.Kazan State Medical Academy – Branch Campus of the FSBEI FPE RMACPE MOH RussiaKazanRussian Federation

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