Synergistic Antimicrobial and Cytotoxic Potential of Zinc Oxide Nanoparticles Synthesized Using Cassia auriculata Leaf Extract
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The problem of microbial resistance is growing, and search for novel approaches to tackle the problem on multidrug resistance pathogens is the need of the hour. The present investigation involves green synthesis of zinc oxide nanoparticles (ZnO NPs) using Cassia auriculata leaf extract and evaluates its synergistic antimicrobial and cytotoxic effect. The results of various techniques confirmed the formation of ZnO NPs. UV-visible spectrum of ZnO NPs showed maximum peak at 370 nm. The crystalline nature of the ZnO NPs was confirmed by XRD analysis. The SEM analysis revealed that particles were spherical and irregular in shape, and average size of nanoparticles was 68.64 nm. The antimicrobial activity and synergistic antimicrobial activity were evaluated against pathogenic microorganisms. ZnO NPs showed broad spectrum of antimicrobial activity against tested pathogens and enhanced synergistic antimicrobial activity as compared to standard antibiotic. Cytotoxic effect of ZnO NPs was evaluated by MTT assay against HeLa cancer cell line, and ZnO NPs showed dose-dependent cytotoxic activity. The synthesized ZnO NPs possess significant antimicrobial and cytotoxic activity and hence can be used therapeutically as nanomedicine for diagnosis and drug therapy.
KeywordsCassia auriculata Zinc oxide nanoparticles Characterization Synergistic antimicrobial activity Cytotoxic activity
The authors thank the Department of Biosciences (UGC-CAS) for providing excellent research facilities. Ms. Hemali Padalia is thankful to UGC-CAS, and Ms. Pooja Moteriya is thankful to UGC, New Delhi, India, for providing Junior Research Fellowship.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 3.Devi, R. S., & Gayathri, R. (2014). Green synthesis of zinc oxide nanoparticles by using Hibiscus rosa-sinensis. International Journal of Current Engineering and Technology, 4(4), 2444–2446.Google Scholar
- 4.Wang, Z. L. (2004). Zinc oxide nanostructures: growth, properties and applications. Journal of Physics: Condensed Matter, 16(25), 829–858.Google Scholar
- 5.Malaikozhundan, B., Vaseeharan, B., Vijayakumar, S., Pandiselvi, K., Kalanjiam, M. A. R., Murugan, K., & Benelli, G. (2017). Biological therapeutics of Pongamia pinnata coated zinc oxide nanoparticles against clinically important pathogenic bacteria, fungi and MCF-7 breast cancer cells. Microbial Pathogenesis, 104, 268–277.CrossRefGoogle Scholar
- 7.Suresh, D., Nethravathi, P. C., Udayabhanu, R. H., Nagabhushana, H., & Sharma, S. C. (2015). Green synthesis of multifunctional zinc oxide (ZnO) nanoparticles using Cassia fistula plant extract and their photodegradative, antioxidant and antibacterial activities. Materials Science in Semiconductor Processing, 31, 446–454.CrossRefGoogle Scholar
- 8.Azizi, S., Mohamad, R., & Shahri, M. (2017). Green microwave-assisted combustion synthesis of zinc oxide nanoparticles with Citrullus colocynthis (L.) Schrad: characterization and biomedical applications. Molecules. https://doi.org/10.3390/molecules22020301.
- 9.Madan, H. R., Sharma, S. C., Udayabhanu, S. D., Vidya, Y. S., Nagabhushana, H., Rajanaik, H., Anantharaju, K. S., Prashantha, S. C., & Maiya, P. S. (2016). Facile green fabrication of nanostructure ZnO plates, bullets, flower, prismatic tip, closed pine cone: their antibacterial, antioxidant, photoluminescent and photocatalytic properties. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 152, 404–416.CrossRefGoogle Scholar
- 11.Chanda, S., Kaneria, M., & Baravalia, Y. (2012). Antioxidant and antimicrobial properties of various polar solvent extracts of stem and leaves of four Cassia species. African Journal of Biotechnology, 11(10), 2490–2503.Google Scholar
- 13.Padalia, H., & Chanda, S. (2017). Characterization, antifungal and cytotoxic evaluation of green synthesized zinc oxide nanoparticles using Ziziphus nummularia leaf extract. Artificial Cells, Nanomedicine Biotechnology. https://doi.org/10.1080/21691401.2017.1282868.
- 15.Perez, C., Paul, M., & Bazerque, P. (1990). An antibiotic assay by the agar well diffusion method. Acta Biologiae et Medicine Experimentalis, 15, 113–115.Google Scholar
- 19.Moteriya, P., & Chanda, S. (2016). Synthesis and characterization of silver nanoparticles using Caesalpinia pulcherrima flower extract and assessment of their in vitro antimicrobial, antioxidant, cytotoxic, and genotoxic activities. Artificial Cells, Nanomedicine, and Biotechnology. https://doi.org/10.1080/21691401.2016.1261871.
- 20.Vijayakumar, S., Vinoj, G., Malaikozhundan, B., Shanthi, S., & Vaseeharan, B. (2015). Plectranthus amboinicus leaf extract mediated synthesis of zinc oxide nanoparticles and its control of methicillin resistant Staphylococcus aureus biofilm and blood sucking mosquito larvae. Spectrochim. Acta Part A: Molecular Biomolecular Spectroscopy, 137, 886–891.CrossRefGoogle Scholar
- 23.Nagajyothi, P. C., Cha, S. J., Yang, I. J., Sreekanth, T. V. M., Kim, K. J., & Shin, H. M. (2015). Antioxidant and anti-inflammatory activities of zinc oxide nanoparticles synthesized using Polygala tenuifolia root extract. Journal of Photochemistry and Photobiology B: Biology, 146, 10–17.CrossRefGoogle Scholar
- 25.Rao, V. S., Ramana, M. V., Satish, N. N., Anuradha, G., & Nageswari, B. (2013). Bio fabrication and characterization of zinc nanorods from Aloe vera. International Journal of Science and Research, 148–150.Google Scholar
- 27.Raj, F. A. A., & Jayalakshmy, E. (2015). Effect of zinc oxide nanoparticle produce by Zingiber officinale against pathogenic bacterial. Journal of Chemical and Pharmaceutical Sciences, 8(1), 124–127.Google Scholar
- 32.Jayaseelan, C., Rahuman, A. A., Kirthi, A. V., Marimuthu, S., Santhoshkumar, T., Bagavan, A., Gaurav, K., Karthik, L., & Rao, K. V. B. (2012). Novel microbial route to synthesize ZnO nanoparticles using Aeromonas hydrophila and their activity against pathogenic bacteria and fungi. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 90, 78–84.CrossRefGoogle Scholar
- 33.Banoee, M., Seif, S., Nazari, Z. E., Jafari-Fesharaki, P., Shahverdi, H. R., Moballegh, A., Moghaddam, K. M., & Shahverdi, A. R. (2010). ZnO nanoparticles enhanced antibacterial activity of ciprofloxacin against Staphylococcus aureus and Escherichia coli. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 93(2), 557–561.CrossRefGoogle Scholar
- 35.Fayaz, A. M., Balaji, K., Girilal, M., Yadav, R., Kalaichelvan, P. T., & Venketesan, R. (2010). Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against Gram positive and Gram negative bacteria. Nanomedicine: Nanotechnology, Biology and Medicine, 6(1), 103–109.CrossRefGoogle Scholar
- 36.AbdEIhady, M. M. (2012). Preparation and characterization of chitosan/zinc oxide nanoparticles for imparting antimicrobial and UV protection to cotton fabric. International Journal of Carbohydrate Chemistry. https://doi.org/10.1155/2012/840591.
- 40.Ann, L. C., Mahmud, S., Bakhori, S. K. M., Sirelkhatim, A., Mohamad, D., Hasan, H., Seeni, A., & Rahman, R. A. (2014). Antibacterial response of zinc oxide structures against Staphylococcus aureus, Psuedomonas aeruginosa and Streptococcus pyogenes. Ceramics International, 40(2), 2993–3001.CrossRefGoogle Scholar
- 41.Chung, I. I. I.-M., Rahuman, A. A., Marimuthu, S., Kirthi, A. V., Anbarasan, K., & Rajakumar, G. (2015). An investigation of the cytotoxicity and caspase-mediated apoptotic effect of green synthesized zinc oxide nanoparticles using Eclipta prostrata on human liver carcinoma cells. Nanomaterials, 5(3), 1317–1330.CrossRefGoogle Scholar
- 42.George, S., Pokhrel, S., Xia, T., Gilbert, B., Ji, Z., Schowalter, M., Rosenauer, A., Damoiseaux, R., Bradley, K. A., Mädler, L., & Nel, A. E. (2010). Use of a rapid cytotoxicity screening approach to engineer a safer zinc oxide nanoparticle through iron doping. ACS Nano, 4(1), 15–29.CrossRefGoogle Scholar