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Chitosan: Whey Protein Isolate: An Effective Emulsifier for Stabilization of Squalene Based Emulsions

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Abstract

Chitosan, a biocompatible functional polysaccharide, often in conjunction with proteins is being employed as efficient food delivery systems assuring better stability and release properties.In the present study, the formulation of a stable squalene-in-water emulsion was attempted using chitosan-whey protein isolate complex as the wall material. Six different treatments (A–F) of biopolymers were prepared by varying the pH (4.5 and 5.5) and chitosan concentration (0.25, 0.5 and 1%), whereas the concentration of whey protein isolate was kept constant (10%). Emulsions were prepared by high speed homogenization after addition of squalene at the rate of 30% of the total wall material weight (w/w). Emulsions prepared using 1% chitosan, 10% whey protein isolate at pH 5.5 (Treatment F) was found to be significantly stable (p < 0.05) with an emulsion stability index of 97.05 ± 0.10%, lowest particle size and highest zeta potential. Rheological analysis revealed that treatment F had the highest viscosity along with the highest consistency coefficient (K) value of 5.85 ± 0.01. The findings of the study showed that a stable complex prepared by 1% chitosan and 10% whey protein isolate at 5.5 pH can be utilized as a stable mixed delivery system for biologically sensitive lipophilic compounds.

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Acknowledgements

Authors express sincere thanks to Director, CIFT, Cochin for providing necessary facilities and support needed for the research. Authors greatly acknowledge ICAR for granting the financial assistance.

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Authors and Affiliations

  1. Central Institute of Fisheries Technology (CIFT), ICAR, Matsyapuri P.O, Cochin, Kerala, 682029, India

    Lekshmi R. G. Kumar, K. K. Anas, S. C. Tejpal, S. N. Chatterjee, V. K. Vishnu, K. K. Asha, R. Anandan & Suseela Mathew

  2. Biochemistry & Nutrition Division, Central Institute of Fisheries Technology (CIFT), Cochin, India

    Lekshmi R. G. Kumar

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  1. Lekshmi R. G. Kumar
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  2. K. K. Anas
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Correspondence to Lekshmi R. G. Kumar.

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Kumar, L.R.G., Anas, K.K., Tejpal, S.C. et al. Chitosan: Whey Protein Isolate: An Effective Emulsifier for Stabilization of Squalene Based Emulsions. Waste Biomass Valor 11, 3477–3483 (2020). https://doi.org/10.1007/s12649-019-00702-0

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