Solid-State Anaerobic Microbial Ensilage: A Combined Wet Storage and Pretreatment Method for the Bioconversion of Lignocellulosic Biomass


Due to the contradiction between the discontinuity of agricultural production and continuity of industrial processing, the collection, storage and transformation of lignocellulosic biomass has become a basic problem in ensuring its sustainable development and large-scale utilization. Two broad methodologies for storage of LCB, dry and wet storage, are introduced. Process parameters including chemical reactions, microbial growth, temperature, and oxygen content, are analyzed in silage of different stages and with different microbial silage additives for the solid anaerobic treatment. Based on the results, we propose a quality evaluation system, encompassing sensory evaluation, organic acid content, soluble carbohydrate content, pH value and bacterial diversity, which can be used as criteria for judging the success of the microbial silage process. Finally, the research on solid-state anaerobic microbial silage pretreatment in biogas and ethanol conversion is summarized, and an optimized method for highly-efficient biochemical transformation of LCB is proposed based on the available findings.

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Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7



Lignocellulosic biomass


Anaerobic digestion


Water-soluble carbohydrates


Lactic acid bacteria


Dry matter


Scanning electron microscope


Dry corn stover


Biochemical methane potential


Total solids


Volatile solids


Colony forming units


Filter paper unit




Simultaneous saccharification and fermentation


Pig manure


Excess sludge


Hot-washing treatment


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This work was funded by the Doctoral Scientific Research Foundation of Zhengzhou University of Light Industry (No. 0123/13501050066).

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Yang, X., Zhang, Z., Song, L. et al. Solid-State Anaerobic Microbial Ensilage: A Combined Wet Storage and Pretreatment Method for the Bioconversion of Lignocellulosic Biomass. Waste Biomass Valor 11, 3381–3396 (2020).

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  • Biogas
  • Biomass
  • Ensiling
  • Ethanol
  • Wet storage