Effect of Maltodextrin Content and Inlet Temperature on the Powder Qualities of Spray-Dried Pineapple (Ananas comosus) Waste Extract


The study aims to recover bioactive compounds, as well as sugar, from pineapple waste extract (PWE) using spray-drying technology. Maltodextrin is used as encapsulating agent combined with PWE at inlet temperature of 100 °C, 110 °C, 120 °C and 130 °C. The spray-drying process of PWE was repeated at 100 °C inlet temperature using two other carrier agents: gum arabic and starch, to compare its solubility. The total polyphenol content (TPC) in powder samples is significantly reduced by 33%, from 27 to 18 mg/g, as the maltodextrin concentration is increased from 2.5 to 10% (p < 0.05). On the other hand, the ferric-reducing antioxidant power of PWE powders is insignificantly affected by the increase in MD content (p > 0.05). Furthermore, the proteolytic enzyme activity in PWE powders significantly increases, from ~ 5 to ~ 9 U/mL, as the MD content is increased from 2.5 to 10% (p < 0.05). The TPC in powder samples significantly decreases from 29 to 22 mg/g as inlet temperature is increased from 100 to 120 °C (p < 0.05). There is also a significant decrease in polyphenol retention, which is about 21%. The protease enzyme activity in PWE powders significantly decreases as the inlet temperature of spray dryer is increased (p < 0.05). It is observed that starch has the lowest retention among the encapsulating agents. The results show that maltodextrin concentration and inlet temperature significantly affected the polyphenol, sugar content and the enzyme activity. The antioxidant capacity of the powders was not affected by both parameters.

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  1. 1.

    Aliakbarian, B., Paini, M., Alberto, A.: Effect of encapsulating agent on physical-chemical characteristics of olive pomace polyphenols-rich extracts. Chem. Eng. Trans. (2015). https://doi.org/10.1080/00224499.2014.976781

    Article  Google Scholar 

  2. 2.

    Alias, N.H., Abbas, Z.: Preliminary investigation on the total phenolic content and antioxidant activity of pineapple wastes via microwave-assisted extraction at fixed microwave power. Chem. Eng. Trans. (2017). https://doi.org/10.3303/CET1756280

    Article  Google Scholar 

  3. 3.

    American Public Health Association—APHA.: Standard Methods for Examination of Water and Wastewater. American Public Health Association, Washington, DC (2005)

    Google Scholar 

  4. 4.

    AOAC. (2012). AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces. AOAC Official Methods of Analysis. AOAC, Gaithersburg

    Google Scholar 

  5. 5.

    Bhattacherjee, A.K., Tandon, D.K., Dikshit, A.: Antioxidant activity and quality of spray dried aonla powder as affected by storage behavior of juice. J. Sci. Ind. Res. 73(09), 607–612 (2014)

    Google Scholar 

  6. 6.

    Bisharat, G.I., Lazou, A.E., Panagiotou, N.M., Krokida, M.K., Maroulis, Z.B.: Antioxidant potential and quality characteristics of vegetable-enriched corn-based extruded snacks. J. Food Sci. Technol. (2015). https://doi.org/10.1007/s13197-014-1519-z

    Article  Google Scholar 

  7. 7.

    Brien, S., Lewith, G., Walker, A., Hicks, S.M., Middleton, D.: Bromelain as a treatment for osteoarthritis: a review of clinical studies. Evid. Based Complement. Altern. Med. (2004). https://doi.org/10.1093/ecam/neh035

    Article  Google Scholar 

  8. 8.

    Caliskan, G., Dirim, S.N.: The effects of the different drying conditions and the amounts of maltodextrin addition during spray drying of sumac extract. Food Bioprod. Process. (2013). https://doi.org/10.1016/j.fbp.2013.06.004

    Article  Google Scholar 

  9. 9.

    Chaurasiya, R.S., Umesh Hebbar, H.: Extraction of bromelain from pineapple core and purification by RME and precipitation methods. Sep. Purif. Technol. (2013). https://doi.org/10.1016/j.seppur.2013.03.029

    Article  Google Scholar 

  10. 10.

    Chobotova, K., Vernallis, A.B., Majid, F.A.A.: Bromelain’s activity and potential as an anti-cancer agent: current evidence and perspectives. Cancer Lett. (2010). https://doi.org/10.1016/j.canlet.2009.08.001

    Article  Google Scholar 

  11. 11.

    Conesa, C., Seguí, L., Laguarda-Miró, N., Fito, P.: Microwaves as a pretreatment for enhancing enzymatic hydrolysis of pineapple industrial waste for bioethanol production. Food Bioprod. Process. 100, 203–213 (2016). https://doi.org/10.1016/j.fbp.2016.07.001

    Article  Google Scholar 

  12. 12.

    Costa-Silva, T.A., Souza, C.R.F., Oliveira, W.P., Said, S.: Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii. Braz. J. Chem. Eng. (2014). https://doi.org/10.1590/0104-6632.20140314s00002880

    Article  Google Scholar 

  13. 13.

    Cupp-Enyard, C.: Sigma’s non-specific protease activity assay-casein as a substrate. J. Vis. Exp. (2008). https://doi.org/10.3791/899

    Article  Google Scholar 

  14. 14.

    D’Archivio, M., Filesi, C., Di Benedetto, R., Gargiulo, R., Giovannini, C., Masella, R.: Polyphenols, dietary sources and bioavailability. Ann. Ist Super. Sanita 43(4), 348–361 (2007)

    Google Scholar 

  15. 15.

    Datta, C., Dutta, A., Dutta, D., Chaudhuri, S.: Adsorption of polyphenols from ginger rhizomes on an anion exchange resin Amberlite IR-400—study on effect of pH and temperature. Procedia Food Sci. (2011). https://doi.org/10.1016/j.profoo.2011.09.135

    Article  Google Scholar 

  16. 16.

    Deng, G.F., Shen, C., Xu, X.R., Kuang, R.D., Guo, Y.J., Zeng, L.S., et al.: Potential of fruit wastes as natural resources of bioactive compounds. Int. J. Mol. Sci. (2012). https://doi.org/10.3390/ijms13078308

    Article  Google Scholar 

  17. 17.

    Dziedzic, S.Z., Kearsley, M.W.: Handbook of Starch Hydrolysis Products and their Derivatives. Springer, New York. (1995). https://doi.org/10.1007/978-1-4615-2159-4

    Google Scholar 

  18. 18.

    Elridge, J.A., Repko, D., Mumper, R.J.: Retention of polyphenolic species in spray-dried blackberry extract using mannitol as a thermoprotectanttle. J. Med. Food 17, 1064–1069 (2014)

    Article  Google Scholar 

  19. 19.

    Fang, Z., Bhandari, B.: Effect of spray drying and storage on the stability of bayberry polyphenols. Food Chem. (2011). https://doi.org/10.1016/j.foodchem.2011.05.093

    Article  Google Scholar 

  20. 20.

    Georgetti, S.R., Casagrande, R., Souza, C.R.F., Oliveira, W.P., Fonseca, M.J.V.: Spray drying of the soybean extract: effects on chemical properties and antioxidant activity. LWT Food Sci. Technol. (2008). https://doi.org/10.1016/j.lwt.2007.09.001

    Article  Google Scholar 

  21. 21.

    Gomez, F., Pablos, M.: Pineapple waste extract for preventing oxidation in model food systems. J. Food Sci. (2016). https://doi.org/10.1111/1750-3841.13341

    Article  Google Scholar 

  22. 22.

    Hemalatha, R., Anbuselvi, S.: Physicohemical constituents of pineapple pulp and waste. J. Chem. Pharm. Res. 5(2), 240–242 (2013)

    Google Scholar 

  23. 23.

    Hossain, M.A., Rahman, S.M.M.: Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Res. Int. (2011). https://doi.org/10.1016/j.foodres.2010.11.036

    Article  Google Scholar 

  24. 24.

    Huang, K., Zhang, P., Hu, B., Yu, S.: The effect of spray drying on sucrose-glycine caramel powder preparation. J. Sci. Food Agric. 96, 2319–2327 (2016)

    Article  Google Scholar 

  25. 25.

    Ignat, I., Volf, I., Popa, V.I.: A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem. (2011). https://doi.org/10.1016/j.foodchem.2010.12.026

    Article  Google Scholar 

  26. 26.

    Ketnawa, S., Chaiwut, P., Rawdkuen, S.: Pineapple wastes: a potential source for bromelain extraction. Food Bioprod. Process. (2012). https://doi.org/10.1016/j.fbp.2011.12.006

    Article  Google Scholar 

  27. 27.

    Kha, T.C., Nguyen, M.H., Roach, P.D.: Effects of spray drying conditions on the physicochemical and antioxidant properties of the Gac (Momordica cochinchinensis) fruit aril powder. J. Food Eng. (2010). https://doi.org/10.1016/j.jfoodeng.2010.01.016

    Article  Google Scholar 

  28. 28.

    Kuppusamy, S., Thavamani, P., Megharaj, M., Naidu, R.: Bioremediation potential of natural polyphenol rich green wastes: a review of current research and recommendations for future directions. Environ. Technol. Innov. (2015). https://doi.org/10.1016/j.eti.2015.04.001

    Article  Google Scholar 

  29. 29.

    Maurer, H.R.: Bromelain: biochemistry, pharmacology and medical use. Cell. Mol. Life Sci. (2001). https://doi.org/10.1007/PL00000936

    Article  Google Scholar 

  30. 30.

    Medina, J.D., Garcia, H.S.: Pineapple post-harvest operations. Instituto Technologico de Veracruz. Veracruz City, Mexico (2005)

    Google Scholar 

  31. 31.

    Mishra, P., Mishra, S., Mahanta, C.L.: Effect of maltodextrin concentration and inlet temperature during spray drying on physicochemical and antioxidant properties of amla (Emblica officinalis) juice powder. Food Bioprod. Process. (2014). https://doi.org/10.1016/j.fbp.2013.08.003

    Article  Google Scholar 

  32. 32.

    Mong Thu, T.T., Krasaekoopt, W.: Encapsulation of protease from Aspergillus oryzae and lipase from Thermomyces lanuginoseus using alginate and different copolymer types. Agric. Nat. Resour. (2016). https://doi.org/10.1016/j.anres.2016.06.002

    Article  Google Scholar 

  33. 33.

    Munin, A., Edwards-Lévy, F.: Encapsulation of natural polyphenolic compounds; a review. Pharmaceutics. (2011). https://doi.org/10.3390/pharmaceutics3040793

    Article  Google Scholar 

  34. 34.

    Murad, H.: Method of treating dermatological disorders by fruit extracts. US Patent No 6,630,163 B1. USA (2003)

  35. 35.

    Namaldi, A., Çalik, P., Uludag, Y.: Effects of spray drying temperature and additives on the stability of serine alkaline protease powders. Dry. Technol. (2006). https://doi.org/10.1080/07373930600961108

    Article  Google Scholar 

  36. 36.

    Nielsen, S.S.: Phenol-sulfuric acid method for total carbohydrates. In: Nielsen, S.S. (eds.), Food Science Texts Series. Springer, Boston (2010)

    Google Scholar 

  37. 37.

    Pandey, R.K., Manimehalai, N.: Production of instant tea powder by spray drying. Int. J. Agric. Food Sci. Technol. 5, 197–202 (2014)

    Google Scholar 

  38. 38.

    Pang, S.F., Yusoff, M.M., Gimbun, J.: Assessment of phenolic compounds stability and retention during spray drying of Orthosiphon stamineus extracts. Food Hydrocoll. (2014). https://doi.org/10.1016/j.foodhyd.2013.10.022

    Article  Google Scholar 

  39. 39.

    PSA.: Major fruit crops quarterly bulletin. PSA, Manila (2018)

    Google Scholar 

  40. 40.

    Rashad, M.M., Mahmoud, A.E., Ali, M.M., Nooman, M.U., Al-Kashef, A.S.: Antioxidant and anticancer agents produced from pineapple waste by solid state fermentation. Int. J. Toxicol. Pharmacol. Res. (2015). https://doi.org/10.1002/anie.200902762

    Article  Google Scholar 

  41. 41.

    Robert, P., Gorena, T., Romero, N., Sepulveda, E., Chavez, J., Saenz, C.: Encapsulation of polyphenols and anthocyanins from pomegranate (Punica granatum) by spray drying. Int. J. Food Sci. Technol. (2010). https://doi.org/10.1111/j.1365-2621.2010.02270.x

    Article  Google Scholar 

  42. 42.

    Roda, A., Lucini, L., Torchio, F., Dordoni, R., De Faveri, D.M., Lambri, M.: Metabolite profiling and volatiles of pineapple wine and vinegar obtained from pineapple waste. Food Chem. (2017). https://doi.org/10.1016/j.foodchem.2017.02.111

    Article  Google Scholar 

  43. 43.

    Rudra, S.G., Jakhar, N., Kaur, C., Nishad, J.: Food industry waste: mine of nutraceuticals. Int. J. Sci. Environ. Technol. 4(1), 205–229 (2015)

    Google Scholar 

  44. 44.

    Saénz, C., Tapia, S., Chávez, J., Robert, P.: Microencapsulation by spray drying of bioactive compounds from cactus pear (Opuntia ficus-indica). Food Chem. (2009). https://doi.org/10.1016/j.foodchem.2008.09.095

    Article  Google Scholar 

  45. 45.

    Saraswaty, V., Risdian, C., Primadona, I., Andriyani, R., Andayani, D.G.S., Mozef, T.: Pineapple peel wastes as a potential source of antioxidant compounds. In: IOP Conference Series: Earth and Environmental Science. (2017). https://doi.org/10.1088/1755-1315/60/1/012013

  46. 46.

    Seguí, L., Fito, P.: An integrated approach for pineapple waste valorisation. Bioethanol production and bromelain extraction from pineapple residues. J. Clean. Prod. 172, 1224–1231 (2018). https://doi.org/10.1016/j.jclepro.2017.10.284

    Article  Google Scholar 

  47. 47.

    Sengupta, S., Dasgupta, M.: Industrial and clinical applications excluding diagnostic clinical enzymology. Enzymology, 1–22 (2006)

  48. 48.

    Shishir, M.R.I., Chen, W.: Trends of spray drying: a critical review on drying of fruit and vegetable juices. Trends Food Sci. Technol. (2017). https://doi.org/10.1016/j.tifs.2017.05.006

    Article  Google Scholar 

  49. 49.

    Simeon, L.: Philippine fruit production grows 4% in quarter 2 2018. The Philippine Star, p. https://www.philstar.com/business/agriculture/2018 (2018)

  50. 50.

    Siti Roha, A.M., Zainal, S., Noriham, A., Nadzirah, K.Z.: Determination of sugar content in pineapple waste variety N36. Int. Food Res. J. (2013). https://doi.org/10.1021/jp037756f

    Article  Google Scholar 

  51. 51.

    Soares, P.A.G., Vaz, A.F.M., Correia, M.T.S., Pessoa, A., Carneiro-Da-Cunha, M.G.: Purification of bromelain from pineapple wastes by ethanol precipitation. Sep. Purif. Technol. (2012). https://doi.org/10.1016/j.seppur.2012.06.042

    Article  Google Scholar 

  52. 52.

    Sukeksi, L., Sarah, M.: Characterizations and extraction of polyphenols from residual pulp of pink guava as source of antioxidants. ARPN J. Eng. Appl. Sci. 11, 5209–5216 (2016)

    Google Scholar 

  53. 53.

    Sulaiman, S.F., Yusoff, N.A.M., Eldeen, I.M., Seow, E.M., Sajak, A.A.B., Supriatno, Ooi, K.L.: Correlation between total phenolic and mineral contents with antioxidant activity of eight Malaysian bananas (Musa sp.). J. Food Compos. Anal. (2011). https://doi.org/10.1016/j.jfca.2010.04.005

    Article  Google Scholar 

  54. 54.

    Sun-Waterhouse, D., Wadhwa, S.S., Waterhouse, G.I.N.: Spray-drying microencapsulation of polyphenol bioactives: a comparative study using different natural fibre polymers as encapsulants. Food Bioprocess Technol. (2013). https://doi.org/10.1007/s11947-012-0946-y

    Article  Google Scholar 

  55. 55.

    Tang, W.-Q., Li, D.-C., Lv, Y.-X., Jiang, J.-G.: Concentration and drying of tea polyphenols extracted from green tea using molecular distillation and spray drying. Dry. Technol. (2011). https://doi.org/10.1080/07373937.2010.516851

    Article  Google Scholar 

  56. 56.

    Terpinc, P., Čeh, B., Ulrih, N.P., Abramovič, H.: Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts. Ind. Crops Prod. (2012). https://doi.org/10.1016/j.indcrop.2012.02.023

    Article  Google Scholar 

  57. 57.

    Thomson, A.B., Keelan, M., Thiesen, A., Clandinin, M.T., Ropeleski, M., Wild, G.E.: Small bowel review: normal physiology part 1. Dig. Dis. Sci. 46(12):2567–2587 (2001)

    Article  Google Scholar 

  58. 58.

    Tochi, B.N., Wang, Z., Xu, S.Y., Zhang, W.: Therapeutic application of pineapple protease (Bromelain): a review. Pak. J. Nutr. (2008). https://doi.org/10.3923/pjn.2008.513.520

    Article  Google Scholar 

  59. 59.

    Tonon, R.V., Brabet, C., Hubinger, M.D.: Anthocyanin stability and antioxidant activity of spray-dried açai (Euterpe oleracea Mart.) juice produced with different carrier agents. Food Res. Int. (2010). https://doi.org/10.1016/j.foodres.2009.12.013

    Article  Google Scholar 

  60. 60.

    Tunchaiyaphum, S., Eshtiaghi, M.N., Yoswathana, N.: Extraction of bioactive compounds from mango peels using green technology. Int. J. Chem. Eng. Appl. (2013). https://doi.org/10.7763/IJCEA.2013.V4.293

    Article  Google Scholar 

  61. 61.

    Upadhyay, A., Lama, J.P., Tawata, S.: Utilization of pineapple waste: a review. J. Food Sci. Technol. Nepal. (2013). https://doi.org/10.3126/jfstn.v6i0.8255

    Article  Google Scholar 

  62. 62.

    Vieira, F.G.K., Borges, G.D.S.C., Copetti, C., Di Pietro, P.F., Nunes, E., da Costa, E., & Fett, R.: Phenolic compounds and antioxidant activity of the apple flesh and peel of eleven cultivars grown in Brazil. Sci. Hortic. (2011). https://doi.org/10.1016/j.scienta.2011.01.032

    Article  Google Scholar 

  63. 63.

    Volf, I., Ignat, I., Neamtu, M., Popa, V.I.: Thermal stability, antioxidant activity, and photo-oxidation of natural polyphenols. Chem. Pap. (2014). https://doi.org/10.2478/s11696-013-0417-6

    Article  Google Scholar 

  64. 64.

    Wolfe, K., Wu, X., Liu, R.H.: Antioxidant activity of apple peels. J. Agric. Food Chem. (2003). https://doi.org/10.1021/jf020782a

    Article  Google Scholar 

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We would like to thank the various pineapple farms for supporting us in this study. We would also like to thank Department of Science and Technology Engineering Research and Development for Technology, Philippines for giving us the opportunity to do our research and Green Environment Management Systems Incorporated together with Chemical Engineering Laboratory Department of University of San Carlos for letting us use the facilities and equipment for the experiment.

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de Ramos, R.M.Q., Siacor, F.D.C. & Taboada, E.B. Effect of Maltodextrin Content and Inlet Temperature on the Powder Qualities of Spray-Dried Pineapple (Ananas comosus) Waste Extract. Waste Biomass Valor 11, 3247–3255 (2020). https://doi.org/10.1007/s12649-019-00651-8

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  • Pineapple waste extract
  • Maltodextrin
  • Spray-drying
  • Spray-dried powders
  • Inlet temperature