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Microbial Induced Calcium Carbonate Precipitation (MICP) Using Pig Urine as an Alternative to Industrial Urea

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Abstract

Biocalcification by microbial induced CaCO3 precipitation (MICP) (catalyzes by microbial enzyme urease) is a promising field of research in recent decades due to its versatile application in industry. Besides, the animal waste disposal has attracted much attention owing to increasing awareness of environmental protection and resource substitution. Though, the biotechnical application of Pig urine which causes of environmental pollution due to existence of high amount of urea or pig urea (PU), not study yet. Present study, PU was used instead of industrial urea, first time, as the enzymatic substance to produced CaCO3 by four sets of experimental quartz-sand column (Control, A (4 h), B (6 h) and C (12 h)) with different injection time intervals (4 h, 6 h and 12 h), bacterial (Sporosarcina pasteurii, DSM 33) amount, pig urine and with 1.1 (M) CaCl2. The results revealed the precipitation of CaCO3 from PU and CaCl2 in presence of S. pasteurii. The porosity and permeability of the quartz-sand column was noted to decrease with the application of pig urine. The mechanical properties (anti-permeability) of the quartz-sand columns were noticed better, while time interval between (4 h) the application of pig urine and bacterial solution was shorter (CaCO3 formation increase by 43%, compare to control). The XRD and SEM results displayed the CaCO3 formation, which confirmed the feasibility of PU as carbonate source. The findings implied a cost reduction of MICP technology by using pig urine as urea, animal waste mitigation for environmental pollution and resource substitution.

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References

  1. Abo-El-Enein, S.A., Ali, A.H., Talkhan, F.N., Abdel-Gawwad, H.A.: Utilization of microbial induced calcite precipitation for sand consolidation and mortar crack remediation. HBRC J. 8, 185–192 (2012)

    Article  Google Scholar 

  2. Anbu, P., Kang, C.H., Shin, Y.J., So, J.S.: Formations of calcium carbonate minerals by bacteria and its multiple applications. SpringerPlus 5(250), 1–26 (2016)

    Google Scholar 

  3. Cheng, L., Cord-Ruwisch, R.: In situ soil cementation with ureolytic bacteria by surface percolation. Ecol. Eng. 42, 64–72 (2012)

    Article  Google Scholar 

  4. Dagnall, S., Jon, H.L., David, P.: Resource mapping and analysis of farm livestock manures—assessing the opportunities for biomass-to-energy schemes. Bioresour. Technol. 71, 225–234 (2002)

    Article  Google Scholar 

  5. DeJong, J.T., Mortensen, B.M., Martinez, B.C., Nelson, D.C.: Bio-mediated soil improvement. Ecol. Eng. 36, 197–210 (2010)

    Article  Google Scholar 

  6. European Commission Directive 2003/30/EC: European Parliament and of the Council of 8 May 2003 on the Promotion of the Use of Biofuels or Other Renewable Fuels for Transport (2003)

  7. Hammes, F., Verstraete, W.: Key roles of ph and calcium metabolism in microbial carbonate precipitation. Rev. Environ. Sci. Bio/Technol. 1, 3–7 (2002)

    Article  Google Scholar 

  8. Hariharan, M., Varghese, N., Cherian, A.B., Sreenivasan, P.V., Paul, J., Asmy Antony, K.A.: Synthesis and characterisation of CaCO3 (calcite) nano particles from cockle shells using chitosan as precursor. Int. J. Sci. Res. Publ. 4(10), 1–5 (2014)

    Google Scholar 

  9. Harkes, M.P., van Paassen, L.A., Booster, J.L., Whiffin, V.S., van Loosdrecht, M.C.M.: Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecol. Eng. 36, 112–117 (2010)

    Article  Google Scholar 

  10. Holm-Nielsen, J.B., Al Seadi, T., Oleskowicz-Popiel, P.: The future of anaerobic digestion and biogas utilization. Bioresour. Technol. 100, 5478–5484 (2009)

    Article  Google Scholar 

  11. Hsu, C.M., Huang, Y.H., Chen, H.J., Lee, W.C., Chiu, H.W., Maity, J.P., Chen, C.C., Kuo, Y.H., Chen, C.Y.: Green synthesis of nano-Co3O4 by microbial induced precipitation (MIP) process using Bacillus pasteurii and its application as supercapacitor. Mater. Today Commun. 14, 302–311 (2018)

    Article  Google Scholar 

  12. Keiko, S., Tomohiko, Y., Masami, T.: Synthesis of aragonite from calcined scallop shell at ambient temperatures and their morphological characterization by FE-SEM. Shigen-to-Sozai 118(8), 553–558 (2002)

    Article  Google Scholar 

  13. Kenpitts apes. Porosity and Permeability Lab. http://kenpitts.net/apes/earth_systems/2007/porosity_permeability_lab.htm. (2007). Accessed 05 June 2017

  14. Krajewska, B.: Urease-aided calcium carbonate mineralization for engineering applications: a review. J. Adv. Res. (2017). https://doi.org/10.1016/j.jare.2017.10.009

    Google Scholar 

  15. Le Me´tayer-Levrel, G., Castanier, S., Orial, G., Loubie`re, J.-F., Perthuisot, J.-P.: Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony. Sed. Geol. 126, 25–34 (1999)

    Article  Google Scholar 

  16. Lopez-Garcia, P., Kazmierczak, J., Benzerara, K., Kempe, S., Guyot Moreira, F.: Bacterial diversity and carbonate precipitation in the giant microbialites from the highly alkaline Lake Van. Turk. Extrem. 9, 263–274 (2005)

    Article  Google Scholar 

  17. Ma, J.A.: Web-based Spatial Decision Support System for Utilizing Organic Wastes as Renewable Energy Resources in New York State. Ph.D. dissertation. Cornell University (2006)

  18. Mujah, D., Shahin, M.A., Cheng, L.: State-of-the-art review of biocementation by microbially induced calcite precipitation (MICP) for soil stabilization geomicrobiology. Journal 34(6), 524–537 (2017)

    Google Scholar 

  19. Muynck, W.D., Verbeken, K., Belie, N.D., Verstraete, W.: Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone. Ecol. Eng. 36(2), 99–111 (2010)

    Article  Google Scholar 

  20. Nemati, M., Voordouw, G.: Modification of porous media permeability, using calcium carbonate produced enzymatically in situ. Enzym. Microb. Technol. 33, 635–642 (2003)

    Article  Google Scholar 

  21. Okwadha, G.D.O., Li, J.: Optimum conditions for microbial carbonate precipitation. Chemosphere 81, 1143–1148 (2010)

    Article  Google Scholar 

  22. Rong, H., Qian, C.X., Li, L.Z.: Influence of molding process on mechanical properties of sandstone cemented by microbe cement. Constr. Build. Mater. 28, 238–243 (2012)

    Article  Google Scholar 

  23. Rong, H., Qian, C.X., Li, L.Z.: Study on microstructure and properties of sandstone cemented by microbe cement. Constr. Build. Mater. 36, 687–694 (2012)

    Article  Google Scholar 

  24. Rowshanbakht, K., Khamehchiyan, M., Sajedi, R.H., Nikudel, M.R.: Effect of injected bacterial suspension volume and relative density on carbonate precipitation resulting from microbial treatment. Ecol. Eng. 89, 49–55 (2016)

    Article  Google Scholar 

  25. Schwantes-Cezario, N., Medeiros, L.P., De Oliveira Jr., A.G., Nakazato, G., Kobayashi, R.K.T., Toralles, B.M.: Bioprecipitation of calcium carbonate induced by Bacillus subtilis isolated in Brazil. Int. Biodeterior. Biodegrad. 123, 200–205 (2017)

    Article  Google Scholar 

  26. Shirakawa, M.A., Kaminishikawahara, K.K., John, V.M., Kahn, H., Futai, M.M.: Sand bioconsolidation through the precipitation of calcium carbonate by two ureolytic bacteria. Mater. Lett. 65, 1730–1733 (2011)

    Article  Google Scholar 

  27. Stocks-Fischer, S., Galinat, J.K., Bang, S.S.: Microbiological precipitation of CaCO3. Soil Biol. Biochem. 31, 1563–1571 (1999)

    Article  Google Scholar 

  28. Weiner, S., Dove, P.M.: An overview of biomineralization processes and the problem of the vital effect. Rev. Mineral. Geochem. 54(1), 1–29 (2003)

    Article  Google Scholar 

  29. Whiffin, V.S.: Microbial CaCO3 precipitation for the production of biocement. PhD Thesis, Murdoch University, Perth, WA, p162 (2004)

  30. Whiffin, V.S., van Paassen, L.A., Harkes, M.P.: Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol. J. 24(5), 417–423 (2007)

    Article  Google Scholar 

Download references

Acknowledgements

Authors would like to thank Ministry of Science and Technology (Taiwan) for financial support (MOST 105-2811-M-194-014 and MOST 106-2811-M-194-006).

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Authors and Affiliations

  1. Department of Civil Engineering, National Chung-Hsing University, Taichung, 402, Taiwan, Republic of China

    How-Ji Chen & Yi-Hsun Huang

  2. Department of Biotechnology, National Kaohsiung Normal University, No. 116, Heping 1st Rd., Lingya District, Kaohsiung City, 80201, Taiwan, Republic of China

    Chien-Cheng Chen

  3. Department of Earth and Environmental Sciences, National Chung Cheng University, 168 University Road, Ming-Shung, Chiayi County, 62102, Taiwan, Republic of China

    Jyoti Prakash Maity & Chien-Yen Chen

  4. School of Civil Engineering and Surveying and International Centre for Applied Climate Science, University of Southern Queensland, Toowoomba, Australia

    Jyoti Prakash Maity

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  1. How-Ji Chen
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  2. Yi-Hsun Huang
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Correspondence to Jyoti Prakash Maity or Chien-Yen Chen.

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Chen, HJ., Huang, YH., Chen, CC. et al. Microbial Induced Calcium Carbonate Precipitation (MICP) Using Pig Urine as an Alternative to Industrial Urea. Waste Biomass Valor 10, 2887–2895 (2019). https://doi.org/10.1007/s12649-018-0324-8

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  • DOI: https://doi.org/10.1007/s12649-018-0324-8

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