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Production of High Quality Fish Oil by Enzymatic Protein Hydrolysis from Cultured Atlantic Salmon By-Products: Investigation on Effect of Various Extraction Parameters Using Central Composite Rotatable Design

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Abstract

Salmon oil was extracted from cultured Atlantic salmon by-product mix (head, frame and viscera) through enzymatic extraction, with experimental combinations of different enzyme (Sea-B-Zyme L200 enzyme) concentrations (0.09–2.91%) and temperature levels (38–52 °C), generated and analyzed through Central Composite Rotatable design and response surface methodology respectively. The oil content obtained was between 13.09–19.18% of the total weight of the sample, where temperature level was observed as a more significant factor than enzyme concentration and highest oil yield was obtained at enzyme concentration of 2.5% and temperature level 50 °C. Salmon by-product oil predominantly contained mono-unsaturated fatty acids (52.49–54.27%), subsequently poly-unsaturated fatty acids (28.97–30.53%) and saturated fatty acids (14.95–17.91%). The extracted oil was also observed as a potential source of oleic acid, linoleic acid, palmitic acid, palmitoleic acid, stearic acid, vaccenic acid, gondoic acid, α-linoleic acid, DHA, DPA and EPA.

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References

  1. Islam, M.S., Khan, S., Tanaka, M.: Waste loading in shrimp and fish processing effluents: potential source of hazards to the coastal and nearshore environments. Mar. Pollut. Bull. 49(1), 103–110 (2004)

    Article  Google Scholar 

  2. Venugopal, V.: Chapter three—enzymes from seafood processing waste and their applications in seafood processing. In: Se-Kwon, K., Fidel, T. (eds.) Advances in Food and Nutrition Research. Vol 78. pp. 47–69. Academic Press, Cambridge (2016)

    Google Scholar 

  3. Jayasinghe, P., Hawboldt, K.: A review of bio-oils from waste biomass: Focus on fish processing waste. Renewable and sustainable energy reviews. 16(1), 798–821 (2012)

    Article  Google Scholar 

  4. Etxabide, A., Leceta, I., Cabezudo, S., Guerrero, P., de la Caba, K.: Sustainable fish gelatin films: from food processing waste to compost. ACS Sustain. Chem. Eng. 4(9), 4626–4634 (2016)

    Article  Google Scholar 

  5. Okazaki, E., Osako, K.: Isolation and characterization of acid-soluble collagen from the scales of marine fishes from Japan and Vietnam. Food chem. 149, 264–270 (2014)

    Article  Google Scholar 

  6. Yahyaee, R., Ghobadian, B., Najafi, G.: Waste fish oil biodiesel as a source of renewable fuel in Iran. Renew. Sustain. Energy Rev. 17, 312–319 (2013)

    Article  Google Scholar 

  7. Schiedt, K., Leuenberger, F.J., Vecchi, M.: Natural Occurrence of Enantiomeric and meso-Astaxanthin. 5. Ex wild salmon (Salmo salar and Oncorhynchus). Helv. Chim. Acta 64(2), 449–457 (1981)

    Article  Google Scholar 

  8. Hudson, S., Smith, C.: Polysaccharides: chitin and chitosan chemistry and technology of their use as structural materials. In: Biopolymers from renewable resources pp. 96–118. Springer, Berlin (1998)

    Chapter  Google Scholar 

  9. Arvanitoyannis, I.S., Kassaveti, A.: Fish industry waste: treatments, environmental impacts, current and potential uses. Int. J Food Sci. Technol. 43(4), 726–745 (2008)

    Article  Google Scholar 

  10. Wu, T.H., Bechtel, P.J.: Salmon by-product storage and oil extraction. Food. Chem. 111(4), 868–871 (2008)

    Article  Google Scholar 

  11. Mbatia, B., Adlercreutz, D., Adlercreutz, P., Mahadhy, A., Mulaa, F., Mattiasson, B.: Enzymatic oil extraction and positional analysis of ω-3 fatty acids in Nile perch and salmon heads. Process Biochem. 45(5), 815–819 (2010). doi:10.1016/j.procbio.2010.02.010

    Article  Google Scholar 

  12. Linder, M., Fanni, J., Parmentier, M.: Proteolytic extraction of salmon oil and PUFA concentration by lipases. Mar. Biotechnol. 7(1), 70–76 (2005). doi:10.1007/s10126-004-0149-2

    Article  Google Scholar 

  13. Taylor, S.L., King, J.W., List, G.R.: Determination of oil content in oilseeds by analytical supercritical fluid extraction. J. Am. Oil Chem. Soc. 70(4), 437–439 (1993)

    Article  Google Scholar 

  14. Li, H., Pordesimo, L., Weiss, J.: High intensity ultrasound-assisted extraction of oil from soybeans. Food Res Int. 37(7), 731–738 (2004)

    Article  Google Scholar 

  15. Lucchesi, M.E., Chemat, F., Smadja, J.: Solvent-free microwave extraction of essential oil from aromatic herbs: comparison with conventional hydro-distillation. J. Chromatogr. A 1043(2), 323–327 (2004)

    Article  Google Scholar 

  16. Rosenthal, A., Pyle, D., Niranjan, K.: Aqueous and enzymatic processes for edible oil extraction. Enzyme Microb. Technol. 19(6), 402–420 (1996)

    Article  Google Scholar 

  17. Johnson, L.A., Lusas, E.: Comparison of alternative solvents for oils extraction.. J. Am. Oil Chem. Soc. 60(2), 229–242 (1983)

    Article  Google Scholar 

  18. Hara, A., Radin, N.S.: Lipid extraction of tissues with a low-toxicity solvent. Anal. Biochem. 90(1), 420–426 (1978)

    Article  Google Scholar 

  19. Rosenthal, A., Pyle, D.L., Niranjan, K.: Aqueous and enzymatic processes for edible oil extraction. Enzyme Microb. Technol. 19(6), 402–420 (1996). doi:10.1016/S0141-0229(96)80004-F

    Article  Google Scholar 

  20. Wang, L., Weller, C.L.: Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Technol. 17(6), 300–312 (2006)

    Article  Google Scholar 

  21. Sherwin, E.: Oxidation and antioxidants in fat and oil processing. J. Am. Oil Chem. Soc. 55(11), 809–814 (1978)

    Article  Google Scholar 

  22. Dominguez, H., Nunez, M., Lema, J.: Enzymatic pretreatment to enhance oil extraction from fruits and oilseeds: a review. Food chem. 49(3), 271–286 (1994)

    Article  Google Scholar 

  23. Dave, D., Ramakrishnan, V.V., Pohling, J., Cheema, S.K., Trenholm, S., Manuel, H., Murphy, W.: Investigation on oil extraction methods and its influence on omega-3 content from cultured salmon. J. Food Process. Technol. (2014). doi:10.4172/2157-7110.1000401

    Article  Google Scholar 

  24. Copeman, L.A., Parrish, C.C.: Lipids classes, fatty acids, and sterols in seafood from Gilbert Bay, Southern Labrador. J. Agric. Food Chem. 52(15), 4872–4881 (2004)

    Article  Google Scholar 

  25. Parrish, C.C.: Lipids in marine ecosystems. ISRN Oceanogr. (2013). doi:10.5402/2013/604045

    Article  Google Scholar 

  26. Hamoutene, D., Volkoff, H., Parrish, C., Samuelson, S., Mabrouk, G., Mansour, A., Mathieu, A., King, T., Lee, K.: Effect of produced water on innate immunity, feeding and antioxidant metabolism in Atlantic cod (Gadus morhua). In: Produced Water pp. 311–328. Springer, Berlin (2011)

    Chapter  Google Scholar 

  27. Myers, R.H., Montgomery, D.C., Anderson-Cook, C.M.: Response surface methodology: process and product optimization using designed experiments. Wiley, Hoboken (2016)

    MATH  Google Scholar 

  28. Rubio-Rodríguez, N., Sara, M., Beltrán, S., Jaime, I., Sanz, M.T., Rovira, J.: Supercritical fluid extraction of fish oil from fish by-products: a comparison with other extraction methods. J. Food Eng. 109(2), 238–248 (2012)

    Article  Google Scholar 

  29. Ohlson, J.: Processing effects on oil quality. J. Am. Oil Chem. Soc. 53(6), 299–301 (1976)

    Article  MathSciNet  Google Scholar 

  30. Balk, E.M., Lichtenstein, A.H., Chung, M., Kupelnick, B., Chew, P., Lau, J.: Effects of omega-3 fatty acids on serum markers of cardiovascular disease risk: a systematic review. Atherosclerosis. 189(1), 19–30 (2006)

    Article  Google Scholar 

  31. Peet, M., Stokes, C.: Omega-3 fatty acids in the treatment of psychiatric disorders. Drugs 65(8), 1051–1059 (2005)

    Article  Google Scholar 

  32. Rose, D.P., Connolly, J.M.: Omega-3 fatty acids as cancer chemopreventive agents. Pharmacol Ther. 83(3), 217–244 (1999). doi:10.1016/S0163-7258(99)00026-1

    Article  Google Scholar 

  33. Lopez-Huertas, E.: Health effects of oleic acid and long chain omega-3 fatty acids (EPA and DHA) enriched milks. A review of intervention studies. Pharmacol. Res. 61(3), 200–207 (2010). doi:10.1016/j.phrs.2009.10.007

    Article  Google Scholar 

  34. Simopoulos, A.P.: Omega-3 fatty acids in inflammation and autoimmune diseases. J. Am. Coll. Nutr. 21(6), 495–505 (2002)

    Article  Google Scholar 

  35. Samieri, C., Féart, C., Proust-Lima, C., Peuchant, E., Tzourio, C., Stapf, C., Berr, C., Barberger-Gateau, P.: Olive oil consumption, plasma oleic acid, and stroke incidence the three-city study. Neurology 77(5), 418–425 (2011)

    Article  Google Scholar 

  36. Belury, M.A.: Dietary conjugated linoleic acid in health: physiological effects and mechanisms of action 1. Annu. Rev. Nutr. 22(1), 505–531 (2002)

    Article  Google Scholar 

  37. Ma, F., Hanna, M.A.: Biodiesel production: a review. Bioresour. Technol. 70(1), 1–15 (1999)

    Article  Google Scholar 

  38. Imahara, H., Minami, E., Saka, S.: Thermodynamic study on cloud point of biodiesel with its fatty acid composition. Fuel 85(12–13), 1666–1670 (2006). doi:10.1016/j.fuel.2006.03.003

    Article  Google Scholar 

  39. Field, C.J., Blewett, H.H., Proctor, S., Vine, D.: Human health benefits of vaccenic acid. Appl. Physiol. Nutr. Metab. 34(5), 979–991 (2009)

    Article  Google Scholar 

  40. Zhang, J., Jia, S., Liu, Y., Wu, S., Ran, J.: Optimization of enzyme-assisted extraction of the Lycium barbarum polysaccharides using response surface methodology. Carbohydr. Polym. 86(2), 1089–1092 (2011). doi:10.1016/j.carbpol.2011.06.027

    Article  Google Scholar 

  41. Abdulkarim, S., Long, K., Lai, O., Muhammad, S., Ghazali, H.: Some physico-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food. Chem. 93(2), 253–263 (2005)

    Article  Google Scholar 

  42. Sengupta, R., Bhattacharyya, D.K.: Enzymatic extraction of mustard seed and rice bran. J. Am. Oil. Chem. Soc. 73(6), 687–692 (1996). doi:10.1007/bf02517941

    Article  Google Scholar 

  43. Sharma, A., Gupta, M.N.: Ultrasonic pre-irradiation effect upon aqueous enzymatic oil extraction from almond and apricot seeds. Ultrason. Sonochem. 13(6), 529–534 (2006). doi:10.1016/j.ultsonch.2005.09.008

    Article  Google Scholar 

  44. Latif, S., Diosady, L.L., Anwar, F.: Enzyme-assisted aqueous extraction of oil and protein from canola (Brassica napus L.) seeds. Eur. J. Lipid Sci. Technol. 110(10), 887–892 (2008)

    Article  Google Scholar 

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Acknowledgements

Authors would like to thank RDC’s Ignite R&D program for providing the funding and continuous support to the project. Authors are grateful to Ms. Heather Manuel for her insight and her expertise in the project. Authors would also like to thank Ms. Sheila Trenholm (Laboratory Technologist, CASD, Marine Institute), Ms. Julia Pohling (Marine Biotechnologist, CASD, Marine Institute) and Jeanette Wells (Research Assistant, Aquatic Research Cluster, Memorial University of Newfoundland) for their expertise and support while conducting the research for their technical support while conducting the research.

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Authors and Affiliations

  1. Marine Bioprocessing Facility, Centre of Aquaculture and Seafood Development, Marine Institute, Memorial University, St. John’s, NL, Canada

    Winny Routray, Deepika Dave, Vegneshwaran V. Ramakrishnan & Wade Murphy

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  1. Winny Routray
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  2. Deepika Dave
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  3. Vegneshwaran V. Ramakrishnan
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Correspondence to Deepika Dave.

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Routray, W., Dave, D., Ramakrishnan, V.V. et al. Production of High Quality Fish Oil by Enzymatic Protein Hydrolysis from Cultured Atlantic Salmon By-Products: Investigation on Effect of Various Extraction Parameters Using Central Composite Rotatable Design. Waste Biomass Valor 9, 2003–2014 (2018). https://doi.org/10.1007/s12649-017-9998-6

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  • DOI: https://doi.org/10.1007/s12649-017-9998-6

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