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Extraction and Characterisation of Collagen from the Skin of Golden Carp (Probarbus Jullieni), a Processing By-Product

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Abstract

Purpose

Fish skin is a by-product generated during fish processing. Nowadays the farmed fish, including golden carp, has gained increasing interest from consumers. As a consequence, a huge amount of skin is generated. Those skins can serve as an important source of collagen, which can be used for several applications in food, pharmaceutical and biomedical industries. Additionally, collagen with high market value can increase the revenue for the farmer or fish processor. This study aimed to extract and characterise collagen from skin of golden carp.

Methods

Acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were isolated from the skin of golden carp (Probarbus jullieni). Obtained collagens were subjected to SDS–PAGE and amino acid analysis. Structural integrity was determined via FTIR and CD spectra. DSC and solubility were also examined.

Results

Both ASC and PSC were characterised as type I collagen. Imino acid contents of ASC and PSC were 197 and 199 residues/1000 residues, respectively. Glycine constituted approximately 1/3 of total amino acid residues. No cysteine was present, indicating the absence of disulphide bonds. FTIR and CD spectra were almost similar between ASC and PSC. Thus pepsin hydrolysis had no marked effect on triple helical structure. ASC and PSC showed higher Tmax values 36.28 and 37.87 °C, respectively, as compared to those from temperate and cold water fish collagens. The maximum solubility for both collagens was found at pH 3.

Conclusion

Skin of golden carp, a by-product from fish processing, could therefore serve as an alternative source of high quality collagen. Subsequently the economic value of fish skin could be maximised and disposal problem could be reduced.

Graphical Abstract

Overview on production and properties of acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from skin of golden carp (Probarbus Jullieni).

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Acknowledgements

The authors would like to express their sincere thanks to the Grant-in-Aid for dissertation from Graduate School, Prince of Songkla University, Thailand. National Research Council of Thailand and TRF Distinguished Research Professor grant were also acknowledged for financial support.

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Authors and Affiliations

  1. Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Ali Muhammed Moula Ali & Soottawat Benjakul

  2. Department of Material and Product Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Thaummanoon Prodpran

  3. Laboratory of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    Hideki Kishimura

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  1. Ali Muhammed Moula Ali
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  2. Soottawat Benjakul
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Correspondence to Soottawat Benjakul.

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Ali, A.M.M., Benjakul, S., Prodpran, T. et al. Extraction and Characterisation of Collagen from the Skin of Golden Carp (Probarbus Jullieni), a Processing By-Product. Waste Biomass Valor 9, 783–791 (2018). https://doi.org/10.1007/s12649-017-9841-0

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