Effects of Surfactant on the Enzymatic Degradation of Oil Palm Empty Fruit Bunch (OPEFB)
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Various pretreatments have been employed to pretreat oil palm empty fruit bunch (OPEFB) to improve sugars production via enzymatic degradation reaction. However, less attention has been paid to investigate the effects of surfactant addition in the OPEFB degradation process. Therefore, the effects of surfactants on degradation of pretreated OPEFB by Aspergillus niger EFB1 crude cellulase were studied. Tween 80 was the most effective surfactant tested. When 0.25% (v/v) Tween 80 was added, the production of reducing sugar increased 63% (from 0.49 to 0.8 g/L). This was the optimum yield recorded. It also improved cellobiose, glucose and xylose production by two folds, seven folds and one fold higher than that without Tween 80, respectively. Tween 80 reduced the loss of cellulases activities where more than 17% of the original cellulases activities were retained after 7 days of degradation. Nonetheless, Tween 80 did not improve the protein concentration consistently. Supplementation of Tween 80 increased lignin removal by 23%. Observations using SEM revealed that, with Tween 80, the presence of pores and surface cracks were more pronounced on the surface of degraded OPEFB fibers. As a whole, the reported effects showed an improved production of reducing sugars in the presence of Tween 80. Thus, Tween 80 addition appeared as a promising method in enhancing the bioconversion of OPEFB into value-added co-products.
KeywordsTween 80 Biodegradation Cellulose Lignin Sugar Cellulase activity
This work was funded by the Ministry of Science, Technology & Environment of Malaysia (MOSTI) (Project No. 73717). The authors also acknowledge Universiti Teknologi Malaysia (UTM) for providing necessary laboratory equipment and facilities important to this work. Noratiqah Kamsani would like to thank Ministry of Higher Education, Malaysia for awarding her the MyBrain 15 (MyPhD) scholarship.
- 16.Yang, M., Zhang, J., Kuittinen, S., Vepsäläinen, J., Soininen, P., Keinänen, M., Pappinen, A.: Enhanced sugar production from pretreated barley straw by additive xylanase and surfactants in enzymatic hydrolysis for acetone–butanol–ethanol fermentation. Bioresour. Technol. 189, 131–137 (2015)CrossRefGoogle Scholar
- 25.Basri, S.A.M.: Degradation of Oil Palm Lignocellulosic Biomass to Cellobiose Through Enzymatic Systems. Universiti Teknologi Malaysia, Skudai (2012)Google Scholar
- 26.Noratiqah, K., Madihah, M., Aisyah, B.S., Eva, M.S., Suraini, A., Kamarulzaman, K.: Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1. Biochem. Eng. J. 75, 8–20 (2013)CrossRefGoogle Scholar
- 31.Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1), 265–275 (1951)Google Scholar
- 32.Goering, H.K., Van Soest, P.J.: Forage fiber analyses (apparatus, reagents, prcedures, and some applications). U.S. Department of Agriculture Handbook, Washington D.C (1970)Google Scholar
- 33.Yao, R., Qi, B., Deng, S., Liu, N., Peng, S., Cui, Q.: Use of surfactants in enzymatic hydrolysis of rice straw and lactic acid production from rice straw by simultaneous saccharification and fermentation. Bioresources 2(3), 389–398 (2007)Google Scholar
- 42.Kim, W., Gamo, Y., Sani, Y.M., Wusiman, Y., Ogawa, S., Karita, S., Goto, M.: Effect of Tween 80 on hydrolytic activity and substrate accessibility of carbohydrolase I (CBH I) from Trichoderma viride. Asian Australas. J. Anim. Sci. 19(5), 684 (2006)Google Scholar
- 44.Castoldi, R., Bracht, A., de Morais, G.R., Baesso, M.L., Correa, R.C.G., Peralta, R.A., Moreira, R.d.F.P.M., de Moraes, M.d.L.T., de Souza, C.G.M., Peralta, R.M.: Biological pretreatment of Eucalyptus grandis sawdust with white-rot fungi: Study of degradation patterns and saccharification kinetics. Chem. Eng. J. 258, 240–246 (2014)CrossRefGoogle Scholar