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Detection of incidence of Babesia spp. in sheep and goats by parasitological diagnostic techniques

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Abstract

The present study was undertaken on epidemiology and diagnosis of babesiosis in sheep and goats in Bengaluru Urban and Rural districts of Karnataka state from November 2017 to May 2018. Out of 343 (225 sheep and 118 goats) blood smears examined by Giemsa and acridine orange (AO) fluorescent dye staining methods, 3.55 and 4.0 per cent of sheep and 0.84 and 1.69 per cent of goat samples were found positive for Babesia organisms, respectively. The sensitivity and specificity was found to be higher in AO fluorescent dye staining method. In agewise susceptibility, the percent positivity was found to be higher in animals > 6 months old. In genderwise susceptibility, the percent positivity was found to be higher in females than males. Hence, AO fluorescent dye staining method is found to be very rapid and cost effective diagnostic method for treatment and control of babesiosis.

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References

  • Achar SD, Sreekantaiah GN (1934) A note on Babesia motasi Wenyon (1926) in sheep in Mysore state Indian. Vet J 10:29–31

    Google Scholar 

  • Aktas M, Altay K, Dumanli N (2007) Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction. Parasitol Res 100:797–802

    Article  PubMed  Google Scholar 

  • Ali Shah SS, Khan MI, Rahman HU (2017) Epidemiological and hematological investigations of tick-borne diseases in small ruminants in Peshawar and Khyber Agency, Pakistan. J Adv Parasitol 4(1):15

    Google Scholar 

  • Altay K, Aktas M, Dumanli N (2008) Detection of Babesia ovis by PCR in Rhipicephalus bursa collected from naturally infested sheep and goats. Res Vet Sci 85:116–119

    Article  CAS  PubMed  Google Scholar 

  • Bai Q, Liu G, Liu D, Ren J, Li X (2002) Isolation and preliminary characterization of a large Babesia spp. from sheep and goats in the eastern part of Gansu Province, China. Parasitol Res 88:S16–S21

    Article  PubMed  Google Scholar 

  • Bazmani A, Abolhooshyar A, Imani-Baran A, Akbari H (2018) Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran. Vet World 11(3):268–273

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bose R, Jorgensen WK, Dalgliesh RJ, Friedhoff KT, De Vos AJ (1995) Current state and future trends in the diagnosis of babesiosis. Vet Parasitol 57:61–74

    Article  CAS  PubMed  Google Scholar 

  • Christensson D, Thunegard E (1981) Babesia motasi in sheep on the island of Gotland in Sweden. Vet Parasitol 9:99–106

    Article  CAS  PubMed  Google Scholar 

  • FAO (Food and Agriculture Organization) (1984) Ticks and tick-borne disease control. A practical field manual, vol 1, Tick Control. FAO, Rome, pp 299

  • Hansen WD, Hunter DT, Richards DE, Allred L (1970) Acridine orange in the staining of blood parasites. Parasitol 56:386–387

    Article  CAS  Google Scholar 

  • Haq AU, Tufani N, Gugjoo MB, Nabi SU, Malik HU (2017) Therapeutic amelioration of severely anaemic local Kashmiri goats affected with babesiosis. Adv Anim Vet Sci 5(11):463–467

    Article  Google Scholar 

  • Jagannath MS, Hedge KS, Shivaram K, Nagaraja KV (1974) An outbreak of babesiosis in sheep and goats and its control. Mysore J Agri Sci 8:441–443

    Google Scholar 

  • Kaufmann J (1996) Text book of parasitic infections of domestic animals. Birkhauser Verlag, Postfach 133, CH-4010 Basel, Schweiz. pp 169–170

  • Lestoquard F (1925) Troisieme note sur les piroplamoses du mouton en Algerie La piroplasmosevraie: Piroplasma (s.sstr.) ovis (n. sp.). Comparison Babesiella ovis. Bull Soc Pathol Exot 18:140

  • Levine ND (1985) Veterinary protozoology. 1st edition. Iowa State University Press, Ames pp 306–309

  • Madhav AKR (1966) Some observations on Babesia motasi infection in sheep in Andhra Pradesh. Indian Vet J 43:785–789

    Google Scholar 

  • Minjauw B, Mcleod A (2003) Tick-borne diseases and poverty. The impact of ticks and tick-borne diseases on the livelihood of small scale and marginal livestock owners in India and eastern and southern Africa. Research report, DFID Animal Health Programme, Centre for Tropical Veterinary Medicine, University of Edinburgh, UK

  • Muraleedharan K, Ziauddin KS, Margoob HP, Puttabytappa B, Seshadri SJ (1994) Prevalence of parasitic infection among small domestic animals. Karnataka J Agric Sci 7:64–68

    Google Scholar 

  • Muthuramalingam T, Pothiappan P, Gnanaraj P, Tensingh Meenakshisundaram S, Pugazhenthi TR, Parthiban S (2014) Report on an outbreak of babesiosis in Tellicherry Goats. Indian J Vet Anim Sci Res 43:58–60

    Google Scholar 

  • Naderi A, Nayebzadeh H, Gholami S (2017) Detection of Babesia infection among human, goats and sheep using microscopic and molecular methods in the city of Kuhdasht in Lorestan Province, West of Iran. J Parasit Dis 41(3):837–842

    Article  PubMed  PubMed Central  Google Scholar 

  • Nair AS, Ravindran R, Lakshmanan B, Kumar SS, Tresamol PV, Saseendranath MR, Senthilvel K, Rao JR, Tewari AK, Ghosh S (2011) Haemoprotozoa of cattle in Northern Kerala, India. Trop Biomed 28(1):68–75

    CAS  PubMed  Google Scholar 

  • Papadopoulos B, Brossard M, Perie NM (1996) Piroplasms of domestic animals in the Macedonia region of Greece. Piroplasms of small ruminants. Vet Parasitol 63(1–2):67–74

    Article  CAS  PubMed  Google Scholar 

  • Prabhakar KS (1976) Studies on theileriosis of sheep with special reference to its prevalence in Karnataka and haematology in splenectomized carriers. M. V. Sc. Thesis, University of Agricultural Sciences, Bangalore

  • Ravindran R, Lakshmanan B, Sreekumar C, John L, Gomathinayagam S, Mishra AK, Tewari AK, Rao JR (2007) Acridine orange staining for quick detection of blood parasites. J Vet Parasitol 21:85–86

    Google Scholar 

  • Ray HN, Raghavachari K (1941) Observations on Babesia foliata sp. from a sheep. Indian J Vet Sci 11:239–242

    Google Scholar 

  • Razmi GR, Naghibi A, Aslani MR, Dastjerdi K, Hossieni H (2003) An epidemiological study on Babesia infection in small ruminants in Mashhad suburb, Khorasan Province, Iran. Small Rum Res 50:39–44

    Article  Google Scholar 

  • Reijbei MR, Gharbi M, Mhadhbi M, Mabrouk W, Ayar B, Nasfi I, Jedidi M, Sassi L, Rekik M, Darghouth MA (2014) Prevalence of piroplasms in small ruminants in North-West Tunisia and the first genetic characterisation of Babesia ovis in Africa. Parasite 21:23–31

    Article  Google Scholar 

  • Ried JF, Armour J, Jennings FW, Urquhart GM (1976) Babesia in sheep: first-isolation. Vet Rec 99:419

    Article  Google Scholar 

  • Sarwar SM (1935) A hitherto undescribed piroplasm of goats (Piroplasma taylori). Indian J Vet Sci Anim Husb 5:71–176

    Google Scholar 

  • Sevinc F, Sevinc M, Ekici OD, Yildiz R, Isik N, Aydogdu U (2013) Babesia ovis infections: detailed clinical and laboratory observations in the pre and post treatment periods of 97 field cases. Vet Parasitol 191:35–43

    Article  PubMed  Google Scholar 

  • Shayan P, Hooshmand E, Nabian S, Rahbari S (2008) Biometrical and genetical characterization of large Babesia ovis in Iran. Parasitol Res 103:217–221

    Article  PubMed  Google Scholar 

  • Soulsby EJL (1982) Helminths, arthropods and protozoa of domesticated animals, 7th edn. Elsevier, New Delhi, p 763

    Google Scholar 

  • Thomson JG, Hall GN (1933) The occurrence of Babesia motasi Wenyon, 1926, in sheep in northern Nigeria, with a discussion on the classification of the piroplasms. J Comp Pathol Ther 46:218–231

    Article  Google Scholar 

  • Vidya MK, Puttalakshamma GC, Halbmandge SC, Kasaralikar VK, Bhoyar R, Patil NA (2011) Babesia ovis infection in goat—a case report. 29th convention and veterinary symposium, ISVM, Feb. 17–19th 2011, Department of Veterinary Medicine, Mumbai Veterinary College, MAFSU, Mumbai, 400 012, pp 35

  • Yeruham I, Hadani A, Galker F, Rosen S (1996) The seasonal occurrence of ticks (Acari: Ixodidae) on sheep and in the field in the Judean area of Israel. Exp Appl Acarol 20:47–56

    CAS  PubMed  Google Scholar 

  • Yin H, Leonhard S, Luo J, Seitzer U, Ahmed JS (2007) Ovine theilerioisis in China: a new look at an old story. Parasitol Res 101(2):191–195

    Article  Google Scholar 

  • Zangana IK, Naqid IA (2011) Prevalence of piroplasmosis (Theileriosis and Babesiosis) among goats in Duhok Governorate. J Vet Sci 4(2):50–57

    Google Scholar 

Download references

Acknowledgements

The facilities provided to carry out research work through Centre of Advanced Faculty Training, ICAR, New Delhi is greatfully acknowledged.

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Authors and Affiliations

  1. Department of Veterinary Parasitology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bengaluru, Karnataka, 560 024, India

    Shakuntala Kage, G. S. Mamatha, Jaya Nagappa Lakkundi & Placid Eugene D’Souza

  2. Department of Parasitology, Veterinary College, Bengaluru, Karnataka, India

    Shakuntala Kage, G. S. Mamatha, Jaya Nagappa Lakkundi & Placid Eugene D’Souza

  3. Institute of Animal Health and Veterinary Biologicals, Bengaluru, Karnataka, India

    B. P. Shivashankar

Authors
  1. Shakuntala Kage
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  2. G. S. Mamatha
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  3. Jaya Nagappa Lakkundi
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  4. B. P. Shivashankar
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  5. Placid Eugene D’Souza
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Contributions

SK, GSM and PED conceived of or designed study. SK performed research. SK, JNL and SS analyzed data. SK and GSM contributed new methods or models. SK and GSM wrote the paper.

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Correspondence to G. S. Mamatha.

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All the authors declare that they have no conflict of interest.

Ethical approval

The work was carried out in a Teaching Veterinary Clinical Hospital and Research Institute, Veterinary College, Bengaluru, Karnataka Veterinary, Animal and Fisheries Sciences University (KVAFSU), Bidar, India. The KVAFSU 2004 Act legislates collection of clinical materials for the diagnosis and treatment of animals.

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Kage, S., Mamatha, G.S., Lakkundi, J.N. et al. Detection of incidence of Babesia spp. in sheep and goats by parasitological diagnostic techniques. J Parasit Dis 43, 452–457 (2019). https://doi.org/10.1007/s12639-019-01109-3

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  • DOI: https://doi.org/10.1007/s12639-019-01109-3

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