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In-vitro and in silico efficacy of isolated alkaloid compounds from Rauvolfia tetraphylla L. against bovine filarial parasite Setaria cervi: a drug discovery approach

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Abstract

Bioassay guided isolation from the leaves of Rauvolfia tetraphylla L. resulted in the isolation and characterization of three compounds of alkaloid in nature namely, Curan-17-oic acid (F1); 18, 19-Secoyohimban (F2) and Reserpiline (F3). Macrofilaricidal activity of three compounds was tested against bovine filarial parasite Setaria cervi using in vitro assays and supported by in silico docking analysis on glutathione-S-transferase (GST) enzyme of Wuchereria bancrofti. All the molecules inhibited GST enzyme to some extent 35.78%, 78.22% and 64.21% respectively. Results were supported by molecular docking studies, which showed docking scores for compound F1 (− 5.14), compound F2 (− 7.19) and compound F3 (− 7.2) on GST enzyme. Thus, in conclusion the in vitro and in silico studies indicated that isolated compounds are promising, inexpensive and widely available natural leads, which can be designed and developed into the macrofilaricidal drugs.

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Fig. 1
Fig. 2

Notes: Vertical lines show mean ± SD. All the values obtained are statistically significant at p < 0.05 when compared with their respective controls. Parasites treated with DMSO were taken as control from which inhibition percentage was calculated

Fig. 3

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Acknowledgements

The authors want to thank the Forest and Environment Dept., Govt. of Odisha for providing funding support in the form of state plan project for smooth completion of research work.

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Authors and Affiliations

  1. Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Odisha, 751015, India

    Dipti Ranjan Behera & Sunita Bhatnagar

Authors
  1. Dipti Ranjan Behera
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  2. Sunita Bhatnagar
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Contributions

DRB contributed in collecting plant samples and parasite, isolated the compounds and performed all in vitro and in silico tests on parasites. SB contributed in data interpretation and critical reading of the manuscript.

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Correspondence to Dipti Ranjan Behera.

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Behera, D.R., Bhatnagar, S. In-vitro and in silico efficacy of isolated alkaloid compounds from Rauvolfia tetraphylla L. against bovine filarial parasite Setaria cervi: a drug discovery approach. J Parasit Dis 43, 103–112 (2019). https://doi.org/10.1007/s12639-018-1064-1

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