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Molecular identification of two cestodes species parasitizing freshwater fishes in India

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Abstract

In the present study, molecular identification of two species of cestodes, Lytocestus indicus and Senga lucknowensis infecting freshwater fishes Clarias magur and Channa punctata, respectively in Manipur is carried out. To ascertain the taxonomic status of these helminth parasites, 18S gene marker was used. Phylogenetic analysis of 18S of Lytocestus sp. showed that it claded with L. indicus from Indian Isolate with a sequence similarity index of 99%. In case of Senga sp., the phylogenetic analysis revealed that it formed a separate clade with S. lucknowensis and Senga vishakapatnamensis, and the sequence similarity index showed maximum homogeneity with S. lucknowensis i.e., 99.8%. Thus, molecular characterization revealed that the two species of cestodes belong to L. indicus and S. lucknowensis.

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Acknowledgements

The authors thankfully acknowledge the Departmental Special Assistance programme of University Grants Commission, New Delhi, to the Department of Zoology, North-Eastern Hill University for providing infrastructural support. The authors would also like to thank UGC for financial support as fellowship to Philayung Zimik.

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  1. Department of Zoology, North-Eastern Hill University, Shillong, Meghalaya, 793022, India

    Philayung Zimik & Bishnupada Roy

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  1. Philayung Zimik
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  2. Bishnupada Roy
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BR framed and designed the experiment and wrote the manuscript. PZ carried out the experiment and wrote the manuscript.

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Correspondence to Bishnupada Roy.

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Zimik, P., Roy, B. Molecular identification of two cestodes species parasitizing freshwater fishes in India. J Parasit Dis 43, 59–65 (2019). https://doi.org/10.1007/s12639-018-1058-z

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