Immunodiagnosis as an aid for early detection of Fasciola gigantica by glutathione S-transferase (GST)

Abstract

In the present study, effort to isolate purified GST from Fasciola gigantica homogenate and its early detection for confirmation of infection has been successful. In this study, immunoprotection studies with GST and antibody titer post-infection has revealed GST to be an immunogenic protein with high sensitivity to detect anti-Fasciola antibody as early as two weeks post-infection in large ruminants.

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References

  1. Dalton JP, Mc Gonigle S, Rolph TP, Andrews SJ (1996) Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin L, proteinases and hemoglobin. Infect Immun 64:5066–5074

    PubMed  CAS  Google Scholar 

  2. Dumenigo BE, Espino AM, Finlay CM, Mezo M (1999) Kinetics of antibody based antigen detection in serum and feces of sheep experimentally infected with Fasciola hepatica. Vet Parasitol 89:153–161

    Article  Google Scholar 

  3. Engvall E, Perlman P (1971) Enzyme linked immunosorbent assay (ELISA). Quantitative assay of IgG. Immunochemistry 8:871–874

    PubMed  Article  CAS  Google Scholar 

  4. Fagbemi BO, Obarisiagbon IO (1991) Common antigens of Fasciola gigantica, Dicrocoelium hospes and Schistosoma bovis and their relevance to serology. Vet Q 13:81–87

    PubMed  Article  CAS  Google Scholar 

  5. Habig WH, Jacoby WB (1981) Assays for differentiation of glutathione S-transferase. Methods Enzymol 77:398

    PubMed  Article  CAS  Google Scholar 

  6. Hillyer GV, De Galanes MS, Battisti G (1992) Fasciola gigantica: host responders and non-responders to parasite glutathione S-transferase. Exp Parasitol 75:176–186

    PubMed  Article  CAS  Google Scholar 

  7. Oldham G (1983) Protection against Fasciola hepatica in rats with adult fluke antigen in Freund’s adjuvant: influence of antigen batch, antigen dose and number sensitizing injections. Res Vet Sci 31(2):240–244

    Google Scholar 

  8. Richard LG (1995) Development and application of a dot-ELISA test for the detection of serum antibody to Fasciola hepatica antigen in lambs. Vet Parasitol 58:9–15

    Article  Google Scholar 

  9. Sarkar S, Mandal S, Manna AK, Roy S, Joardar SN, Dasgupta CK (2003) Detection of anti-Fasciola antibodies in cattle and buffalo sera using DID and ELISA. Indian J Animal Health 42(1):26–30

    Google Scholar 

  10. Soulsby EJL (1982) Helminths, arthropods and protozoa of domesticated animals. ELBS and Bailliere Tyndall, London, p 809

    Google Scholar 

  11. Swarup D, Pachauri SP, Sharma B (1987) Comparative efficacy of serodiagnosis test in buffalo naturally infected with F. gagantica by ELISA. Indian J Animal Sci 57:373–376

    Google Scholar 

  12. Wijfell GL, Sexton JL, Salvatore L, Pettitt JMM, Humphris DC, Panaccio M, Spithill TW (1992) Primary sequence heterogeneity and tissue expression of glutathione S-transferase of Fasciola hepatica. Exp Parasitol 74:87–99

    Article  Google Scholar 

  13. Yokananth S, Ghosh S, Gupta SC, Suresh MG, Saravanan D (2005) Characterization of specific and cross-reacting antigens of Fasciola gigantica by immunoblotting. Parasitol Res. doi:10.1007/S00436-005-1371-1

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Acknowledgments

The authors are thankful to Hon’ble Vice Chancellor, West Bengal University of Animal and Fishery Sciences and the Dean, Faculty of Veterinary and Animal Sciences, WBUAFS for providing the necessary facilities for carrying out this research work.

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Correspondence to S. K. Mukhopadhayay.

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Mandal, S., Mukhopadhayay, S.K., Ganguly, S. et al. Immunodiagnosis as an aid for early detection of Fasciola gigantica by glutathione S-transferase (GST). J Parasit Dis 36, 207–209 (2012). https://doi.org/10.1007/s12639-012-0106-3

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Keywords

  • GST
  • Fasciola gigantica
  • Immunoprotection
  • Immunogenic
  • Anti-Fasciola antibody