Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides of approximately 100 amino acid residues with a broad spectrum of antimicrobial activity. It is an amphipathic peptide with an N-terminal hydrophobic region and a lipopolysaccharide binding domain (LBD). In the present study, we report an isoform of the anti-lipopolysaccharide factor (Mm-ALF) from the speckled shrimp, Metapenaeus monoceros. A 359 bp cDNA encoded 119 amino acids, and the sequence showed 99.16% similarity to ALF from the shrimp Fenneropenaeus indicus. The mature peptide of 94 amino acids has a net charge of +8, molecular weight 10.62 kDa, and pI 10.11. The mature peptide Mm-ALF was recombinantly expressed in E. coli Rosetta-gami cells, and the peptide was isolated and purified. The rMm-ALF exhibited notable antibacterial activity against Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Escherichia coli, Edwardsiella tarda, Aeromonas hydrophila, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio proteolyticus, Vibrio cholerae and Vibrio fluvialis) bacteria.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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The authors are thankful to the Director, Centre for Marine Living Resources and Ecology (CMLRE) and Ministry of Earth Sciences (MoES), Government of India, for the research grant (MoES/10-MLR/01/2012) and scientific support for the work. The first author is grateful to the University Grants Commission (UGC) for the award of the fellowship, the second author to DST, Govt. of India for the WoS-B Grant (NASI/SoRF-1/2015-16/07 dt.20-01-2016) and the corresponding author to UGC, Govt. of India for the BSR Faculty Grant (F.18-1/2011(BSR) dt. 16 May 2019). The authors wish to thank the Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology and National Centre for Aquatic and Animal Health (NCAAH), and Cochin University of Science and Technology for the facilities provided for the successful completion of the work.
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K, A., Sebastian, S.R., K.S., S. et al. Molecular and Functional Characterization of an Anti-lipopolysaccharide Factor Mm-ALF from Speckled Shrimp Metapenaeus monoceros. Probiotics & Antimicro. Prot. (2021). https://doi.org/10.1007/s12602-021-09741-3
- Antimicrobial peptide
- Metapenaeus monoceros
- Anti-lipopolysaccharide factor
- Host defence peptides