Modeling the Ethanol Tolerance of the Probiotic Yeast Saccharomyces cerevisiae var. boulardii CNCM I-745 for its Possible Use in a Functional Beer


Saccharomyces yeasts are able to ferment simple sugars to generate levels of ethanol that are toxic to other yeasts and bacteria. The tolerance to ethanol of different yeasts depends also on the incubation temperature. In this study, the ethanol stress responses of S. cerevisiae and the probiotic yeast S. boulardii CNCM I-745 were evaluated at two temperatures. The growth kinetics parameters were obtained by fitting the Baranyi and Roberts model to the experimental data. The four-parameter logistic Hill equation was used to describe the ethanol tolerance of the yeasts at the temperatures of 28 and 37 °C. Adequate determination coefficients were obtained (R2 > 0.91) in all cases. S. boulardii grown at 28 °C was selected as the yeast with the best ethanol tolerance (6–8%) for use in the elaboration of functional craft beers.

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Author Ramírez-Cota received scholarship from CONACyT (Mexico) to study her PhD program, and all the authors received a grant from the SIP of Instituto Politécnico Nacional, Mexico (grant number 20195505) to develop the thesis project.

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Correspondence to Humberto Hernández-Sánchez.

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Ramírez-Cota, G.Y., López-Villegas, E.O., Jiménez-Aparicio, A.R. et al. Modeling the Ethanol Tolerance of the Probiotic Yeast Saccharomyces cerevisiae var. boulardii CNCM I-745 for its Possible Use in a Functional Beer. Probiotics & Antimicro. Prot. (2020).

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  • Ethanol tolerance
  • Saccharomyces boulardii CNCM I-745
  • Dose-response model
  • Functional craft beer