Pitchers are the unique structures of carnivorous plants used for the trapping of insects and other small invertebrates. The digestion of captured prey here is assisted by the bacteria, which have been associated with pitchers. These bacterial communities can therefore expect to have a variety of plant beneficial functions. In this study, the bacterial isolate NhPBG1 from the pitcher of Nepenthes hamblack was screened for activity against Pythium aphanidermatum, Rhizoctonia solani, Fusarium oxysporum, and Colletotrichum accutatum and was found to have the inhibitory activity towards all the tested phytopathogens. Interestingly, the isolate was found to have hyper-inhibitory effect against P. aphanidermatum. Further to this, the isolate was also shown to be positive for plant beneficial traits such as indole-3-acetic acid (IAA) and ammonia production, phosphate, potassium and zinc solubilization, nitrogen fixation, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. BLAST analysis of the 16S rDNA sequence of NhPBG1 has identified it as Paraburkholderia sp. Also, the Zingiber officinale rhizome pre-treated with NhPBG1 was found to get protected from P. aphanidermatum induced infection, whereas the control showed symptoms of infection. This was further confirmed by the microscopic evaluation of the presence of fungal mycelia in the tissues of control. However, the mycelial invasion could not be detected in the NhPBG1 treated rhizome. The metabolite profiling of NhPBG1 by GC–MS has identified variety of general metabolites, while the antifungal compounds pyocyanin and 1-hydroxyphenazine could be identified by the LC–MS/MS analysis.
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The authors acknowledge Kerala State Plan Fund Project and DST-PURSE P II programme.
This study is supported by the Kerala State Council for Science, Technology and Environment—Kerala Biotechnology Commission—Young Investigators Programme in Biotechnology (673/2017/KSCSTE dated October 13, 2017) and JAIVAM project, Mahatma Gandhi University Kottayam (3972/AD A7/2019 dated August 17, 2019).
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Ravi, A., Theresa, M., Nandayipurath, V.V.T. et al. Plant Beneficial Features and Application of Paraburkholderia sp. NhPBG1 Isolated from Pitcher of Nepenthes hamblack. Probiotics & Antimicro. Prot. (2020). https://doi.org/10.1007/s12602-020-09665-4
- Paraburkholderia sp.
- Nepenthes hamblack
- Rhizome protection
- Biocontrol properties
- Plant beneficial traits
- Zingiber officinale