Enterocin M and Sage Supplementation in Post-weaning Rabbits: Effects on Growth Performance, Caecal Microbiota, Fermentation and Enzymatic Activity


The effects of enterocin (Ent) M and sage extract applied separately and in combination were investigated. EntM (E 50 μL/animal/day in water) and sage extract (S 10 μL/animal/day in water) were applied individually and in combination (E+S) to rabbits during 21 days of treatment. The rabbits’ growth was not significantly influenced by the additives. Lower feed conversion (FC) was noted in the experimental groups compared with controls, with the lowest data detected in E. The antimicrobial activity of EntM was noted (in E+S: lactic acid bacteria—P < 0.01; in E, E+S: enterococci, enterobacteria—P > 0.05; in E: clostridia—P > 0.05). The most significant changes in fermentation between weaned and older rabbits were noted in amylolytic activity at day 21 (E P < 0.05; E + S P < 0.05); prolonged reduction effect of sage extract on amylolytic activity was observed. The activity of cellulase, pectinase and xylanase was higher in older than in younger animals. Decrease in lactic acid and volatile fatty acids was noted during EntM administration, with significant effect on propionic acid concentration (E P < 0.05; E+S P < 0.001). The sage extract reduced propionic acid (S P < 0.001) and butyric acid levels (S P < 0.05) and increased the concentrations of butyric, iso-valeric, valeric, caproic acids and lactic acid (P < 0.001). It seems to be that EntM and sage supplementation may improve the economy of rabbit farms (increased FC) and the health status of rabbits (reduction of spoilage microbiota, enhanced enzymatic activities in caecum).

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  1. 1.

    Gidenne T (1994) Estimation of volattile fatty acids and of their energetic supply in the rabbit caecum: effect of the dietary fibre level. Vleme Journees de la Recherche Cunicole, Paris, pp 293–299

    Google Scholar 

  2. 2.

    Combes S, Fortune-Lamothe L, Cauqui L, Gidenne T (2013) Engineering the rabbit digestive ecosystem to improve digestive health and efficacy. Animal 7(9):1429–1439

    CAS  Article  Google Scholar 

  3. 3.

    Padilha MTS, Licois D, Gidenne T, Carre B, Fonty G (1995) Relationships between microflora and caecal fermenation in rabbits before and after weaning. Repr Nutr Dev 35:375–386

    CAS  Article  Google Scholar 

  4. 4.

    Gidenne T, Fortun-Lamothe L (2002) Feeding strategy for young rabbits around weaning. A review of digestive capacity and nutritional needs. Anim Sci 75:169–184

    CAS  Article  Google Scholar 

  5. 5.

    Pogány Simonová M, Lauková A, Chrastinová Ľ, Strompfová V, Faix Š, Vasilková Z, Ondruška Ľ, Jurčík R, Rafay J (2009) Enterococcus faecium CCM7420, bacteriocin PPB CCM7420 and their effect in the digestive tract of rabbits. Czech J Anim Sci 54:376–386

    Article  Google Scholar 

  6. 6.

    Papatsiros VG, Christodoulopoulos G (2011) The use of organic acids in rabbit farming. Online J Anim Feed Res 1:434–438

    Google Scholar 

  7. 7.

    Szabóová R, Lauková A, Chrastinová Ľ, Strompfová V, Pogány Simonová M, Vasilková Z, Čobanová K, Plachá I, Chrenková M (2011) Effect of combined administration of enterocin 4231 and sage in rabbits. Pol J Vet Sci 14:359–366

    Article  Google Scholar 

  8. 8.

    Lauková A, Chrastinová Ľ, Pogány Simonová M, Strompfová V, Plachá I, Čobanová K, Formelová Z, Chrenková M, Ondruška Ľ (2012) Enterococcus faecium AL 41:its enterocin M and their beneficial use in rabbits husbandry. Probiotics Antimicrob Proteins 4:243–249

    Article  Google Scholar 

  9. 9.

    Dalle Zotte A, Celia C, Zs S (2016) Herbs and spices inclusion as feedstuff or additive in growing rabbit diet and as additive in rabbit meat: a review. Livest Sci 189:82–90

    Article  Google Scholar 

  10. 10.

    Kalma RP, Patel VK, Joshi A, Umatiya RV, Parmar KN, Damor SV, Chauchan HD, Srivastava AK, Sharma HA (2016) Probiotic supplementation in rabbit:a review. Int J Agricult Sci 8:2811–2815

    CAS  Google Scholar 

  11. 11.

    Lauková A, Pogány Simonová M, Chrastinová Ľ, Gancarčíková S, Kandričáková A, Plachá I, Chrenková M, Formelová Z, Ondruška Ľ, Ščerbová J, Strompfová V (2018) Assessment of Lantibiotic type bacteriocin - Gallidermin application in model experiment with broiler rabbits. Int J Anim Sci 2(4):1028

    Google Scholar 

  12. 12.

    Pogány Simonová M, Lauková A, Plachá I, Čobanová K, Strompfová V, Szabóová R, Chrastinová Ľ (2013) Can enterocins affect phagocytosis and glutathione-peroxidase in rabbits? Centr Eur J Biol 8(8):730–734

    Google Scholar 

  13. 13.

    Mareková M, Lauková A, Skaugen M, Nes FI (2007) Isolation and characterization of a new bacteriocins produced by enviromental isolate Enterococcus faecium AL41. J Indust Microbiol Biotechnol 34:533–537

    Article  Google Scholar 

  14. 14.

    De Vuyst L, Callewaert R, Pot B (1996) Characterization of the antagonistic activity of Lactobacillus amylovorus DCE471 and large scale isolation of its bacteriocin amylovorin L471. Syst Appl Microbiol 9:9–20

    Article  Google Scholar 

  15. 15.

    Szabóová R, Lauková A, Chrastinová Ľ, Simonová M, Strompfová V, Haviarová M, Plachá I, Faix Š, Vasilková Z, Chrenková M, Rafay J (2008) Experimental application of sage in rabbit husbandry. Acta Vet Brno 7:581–588

    Article  Google Scholar 

  16. 16.

    Miltko R, Bełżecki G, Kowalik B, Skomiał J (2016) Presence of carbohydrate-digesting enzymes throughout the digestive tract of sheep. Turkish J Vet Anim Sci 40:271–277

    CAS  Article  Google Scholar 

  17. 17.

    Lauková A, Chrastinová Ľ, Plachá I, Kandričáková A, Szabóová R, Strompfová V, Chrenková M, Čobanová K, Žitňan R (2014) Beneficial effect of lantibiotic nisin in rabbit husbandry. Probiotics Antimicrob Proteins 6:41–46

    Article  Google Scholar 

  18. 18.

    Sirotek K, Marounek M, Suchorská O (2006) Activity and cellular localization of amylases of rabbit caecal bacteria. Folia Microbiol 51(4):309–312

    CAS  Article  Google Scholar 

  19. 19.

    Sirotek K, Marounek M, Rada V, Benda V (2001) Isolation and characterization of rabbit caecal pectinolytic bacteria. Folia Microbiol 46:79–82

    CAS  Article  Google Scholar 

  20. 20.

    Gupta P, Samant K, Sahu A (2012) Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential. Int J Microbiol:Article ID 578925 5 pages

  21. 21.

    Marcin A, Levkut M, Revajová V, Šoltysová B, Naď P (2016) Influence of Salvia officinalis essential oil on digestion parameters and intestinal microflora of broiler chickens. Folia Vet 60(1):5–14

    CAS  Article  Google Scholar 

  22. 22.

    Nickavar B, Abolhasani L (2013) Bioactivity-guided separation of an α-amylase inhibitor flavonoid from Salvia virgata. Iran J Pharm Res 12(1):57–61

    CAS  PubMed  PubMed Central  Google Scholar 

  23. 23.

    Lavrenčič A (2007) The effect of rabbit age on vitro caecal fermentation of starch, pectin, xylan, cellulose, compound feed and its fibre. Animal 1:241–248

    Article  Google Scholar 

  24. 24.

    Marounek M, Vovk SJ, Skřivanová V (1995) Distribution of activity of hydrolytic enzymes in the digestive tract of rabbits. Br J Nutr 73:463–469

    CAS  Article  Google Scholar 

  25. 25.

    Phuoc TL, Jamikorn U (2017) Effects of probiotic supplement (Bacillus subtilis and Lactobacillus acidophilus) on feed efficiency, growth performance, and microbial population of weaning rabbits. Asian-Austral J Anim Sci 30(2):198–205

    CAS  Article  Google Scholar 

  26. 26.

    Bovera F, Iannaccone F, Mastellone V, Nizza S, Lestingi A, De Martino L, Lombard P, Mallardo K, Ferrara M, Nizza A (2012) Effect of spray application of Lactobacillus plantarum on in vivo performance, caecal fermentations and haematological traits of suckling rabbits. Ital J Anim Sci 11:145–149

    CAS  Article  Google Scholar 

  27. 27.

    Van Soest PJ (1993) Cell wall matrix interactions and degradation – session synopsis. In: Jung HD, Buxton DR, Hatfield RD, Ralph J (eds) Forage cell wall structuree and digestibility. ASA-CSSA-SSSA Publ, Madison, WI, USA

    Google Scholar 

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We are grateful to Mrs. M. Bodnárová and Mr. P. Jerga for their skillful technical assistance and also to Dr. V. Párkányi, Dr. R. Jurčík and Dr. Ľ. Ondruška from the National Agricultural and Food Centre in Nitra for caecal sampling and Mr. J. Pecho for slaughtering. We would like to thank Mr. A. Billingham for the English correction of the manuscript.


This work was supported by the projects VEGA 2/0006/17 and ITMS 26220220204 of the Research and Development Operational Programme funded by the ERDF.

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Correspondence to M. Pogány Simonová.

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Simonová, M.P., Chrastinová, Ľ., Kandričáková, A. et al. Enterocin M and Sage Supplementation in Post-weaning Rabbits: Effects on Growth Performance, Caecal Microbiota, Fermentation and Enzymatic Activity. Probiotics & Antimicro. Prot. 12, 732–739 (2020). https://doi.org/10.1007/s12602-019-09584-z

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  • Enterocin
  • Sage
  • Microflora
  • Enzymes
  • Organic acids