Study on Antiviral Activity of Two Recombinant Antimicrobial Peptides Against Tobacco Mosaic Virus


Antimicrobial peptides (AMPs) are generally small peptides with less than 50 amino acid residues, which have been considered as the first line of defense system in plants and animals. These small cationic peptides belong to a family of antimicrobials that are multifunctional effectors of innate immunity. The direct antimicrobial activity of AMPs against different bacteria, viruses, fungi, and parasites has been confirmed in different studies. In this study, the antiviral activity of two recombinant AMPs named thanatin and lactoferricin+lactoferrampin was evaluated against Tobacco mosaic virus (TMV) using half-leaf and leaf disk methods under in vivo and in vitro condition, respectively. The obtained result indicated that both recombinant AMPs have shown an antiviral activity against TMV. Compared to the chimeric lactoferricin+lactoferrampin, recombinant thanatin showed a higher rate of antiviral activity against TMV. Three types of effects, including protective, curative, and inactivation, were evaluated during an antiviral activity test. In the present study, the antiviral activity of two recombinant AMPs is represented for the first time: thanatin and chimeric lactoferricin+lactoferrampin against TMV as a viral plant pathogen.

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We thank Dr. Mojtaba Hosseini for his helpful discussion during statistical analysis.


This study was funded by the Deputy of Research and Technology, Ferdowsi University of Mashhad, Mashhad, Iran (Grant No. 43239).

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Correspondence to Mojtaba Mamarabadi.

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Mohammad Ali Sabokkhiz declares that he has no conflict of interest. Abbas Tanhaeian declares that he has no conflict of interest. Mojtaba Mamarabadi declares that he has no conflict of interest.

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Sabokkhiz, M.A., Tanhaeian, A. & Mamarabadi, M. Study on Antiviral Activity of Two Recombinant Antimicrobial Peptides Against Tobacco Mosaic Virus. Probiotics & Antimicro. Prot. 11, 1370–1378 (2019).

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  • Thanatin
  • Lactoferricin
  • Lactoferrampin
  • TMV
  • Plant viruses
  • Disease control