Potential Properties of Lactobacillus plantarum F-10 as a Bio-control Strategy for Wound Infections

Abstract

In this study, Lactobacillus plantarum F-10, a promising probiotic strain isolated from fecal microbiota of healthy breastfed infant, was assessed as a bio-control strategy for wound infections. Pseudomonas aeruginosa PAO1/ATCC 27853, methicillin-resistant Staphylococcus aureus ATCC 43300, and their hospital-derived strains isolated from skin chronic wound samples were used as important skin pathogens. The cell-free extract (CFE) of the strain F-10 was shown to inhibit the growth of all pathogens tested, while no inhibition was observed when CFE was neutralized. The strain displayed no hemolysis and exhibited a strong auto-aggregating phenotype (51.48 ± 1.45%, 5 h) as well as co-aggregation. Antibiotic resistance profile was found to be safe according to the European Food Safety Authority. Biofilm formation was measured by crystal violet assay and visualized with scanning electron microscopy and confocal laser scanning microscopy. One hundred percent reduction in biofilm formation of all pathogens tested was obtained by sub-MIC value (12.5 mg/ml) of CFE following 24-h co-incubation. Inhibition of quorum sensing–controlled virulence factors (motility, protease and elastase activity, production of pyocyanin and rhamnolipid) of P. aeruginosa strains was also observed. DPPH radical scavenging activity of the CFE was determined as 88.57 ± 0.49%. In conclusion, our results suggest that L. plantarum F-10 may represent an alternative bio-control strategy against skin infections with its antimicrobial, anti-biofilm, anti-quorum sensing, and antioxidant activity.

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Acknowledgments

The authors wish to sincerely thank Dr. Basar KARACA (Ankara University) for his excellent technical assistance on biofilm analysis.

Funding

This work was supported by grants of the Scientific Research Project Coordination Unit of Ankara University, Turkey (Project No: 17B0430001).

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Correspondence to Fadime Kiran.

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Onbas, T., Osmanagaoglu, O. & Kiran, F. Potential Properties of Lactobacillus plantarum F-10 as a Bio-control Strategy for Wound Infections. Probiotics & Antimicro. Prot. 11, 1110–1123 (2019). https://doi.org/10.1007/s12602-018-9486-8

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Keywords

  • Lactobacillus plantarum
  • Wound infection
  • Probiotic
  • Biofilm
  • Quorum sensing
  • Antioxidant